Annotating carbohydrate structures to develop markers for consumption of food
注释碳水化合物结构以开发食物消耗标记
基本信息
- 批准号:10543847
- 负责人:
- 金额:$ 60.61万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-04-01 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:16S ribosomal RNA sequencingAmylasesAnimalsArabinoseBiologicalBiological MarkersBiomarker of Dietary IntakeBioreactorsCarbohydratesClinicalClinical NutritionClinical ResearchClinical TrialsComplementConsumptionCoronary heart diseaseDataDietDietary FiberDietary InterventionDietary intakeDigestionElementsEnrollmentEnzymesFecesFermentationFiberFoodFood AnalysisFood InteractionsFreezingFutureGastrointestinal DiseasesGenesGerman populationGoalsHealthHigher Order Chromatin StructureHumanInfantIntakeLibrariesLinkMeasurementMeasuresMetagenomicsMethodsMicrobeModernizationModificationMolecular ProfilingMonitorMonosaccharidesNon-Insulin-Dependent Diabetes MellitusNutritional StudyOligosaccharidesOutcomePlant ComponentsPolysaccharidesPredictive Value of TestsPublic HealthResearchResistanceResolutionRoleScientistSourceStructureTestingToddlerTravelVolatile Fatty AcidsWeaningWorkcarbohydrate databasecarbohydrate structuredietaryfeedingfood consumptionfundamental researchgenetic linkage analysisgood dietgut microbesgut microbiomegut microbiotainnovationmetabolomicsmetatranscriptomicsmicrobialmicrobiomemolecular markermultidisciplinarymultiple omicsnovelnutritionobesity riskpotential biomarkerpredictive markertraining opportunity
项目摘要
Biomarkers of food consumption are important indicators for clinical and feeding studies that establish our understanding of nutrition. Carbohydrates make up the largest component of a healthy diet. Biomarkers that are derived directly from carbohydrates are ideal. However, there is a fundamental research gap in that carbohydrate structures of specific foods that are commonly consumed are unknown. The long-term goal is to determine specific glycan biomarkers of dietary intake for all commonly consumed foods. The objective of the current application is to develop a detailed carbohydrate structure library of foods in terms of their monosaccharide and linkage compositions and to evaluate carbohydrate structures for their utility as biomarkers of dietary intake. Our central hypothesis is specific foods have unique carbohydrate structures that can serve as biomarkers of dietary intake. This hypothesis has been formulated on the basis of preliminary studies in which (a) detailed carbohydrate structures have been determined for hundreds of foods and (b) fecal glycans, microbes, and metabolites differed by dietary intervention of weaning foods in infants. Enzymes in the gut from human and their complement microbes are specific to structural carbohydrate features. Therefore, the specific aims are to (1) construct a glycomic library with paired analytical platform for food and feces, (2) conduct a microbial gene marker library using bioreactor studies and (3) test the predictive value of glycan and microbial biomarkers in a human feeding study. The approach is innovative, bringing unprecedented high-resolution carbohydrate content analysis—monosaccharide and linkage analysis of foods—with multiple omic measurements to biomarker prediction of dietary intake. The proposed research is significant and impactful because a high-resolution glycan library of foods together with glycan-microbe biomarker products of those foods will transform future clinical trials in which diet is an essential component.
食物消耗的生物标志物是临床和喂养研究的重要指标,可帮助我们了解碳水化合物是健康饮食的最大组成部分。然而,这方面存在根本性的研究空白。经常食用的特定食物的碳水化合物结构是未知的,长期目标是确定所有常见食物的饮食摄入量的特定聚糖生物标志物,当前应用的目标是开发一个详细的食物碳水化合物结构库。它们的单糖和连锁组成,并评估碳水化合物结构作为膳食摄入生物标志物的效用,我们的中心假设是特定食物具有独特的碳水化合物结构,可以作为膳食摄入生物标志物。 a) 数百种食物的详细碳水化合物结构已被确定;(b) 粪便聚糖、微生物和代谢物因婴儿断奶食品的饮食干预而有所不同。人类肠道中的酶及其补体微生物对结构碳水化合物具有特异性。因此,具体目标是(1)构建具有食物和粪便配对分析平台的糖组库,(2)利用生物反应器研究建立微生物基因标记库,以及(3)测试聚糖和微生物预测生物标记物的价值。该方法是创新的,将高分辨率碳水化合物含量分析(单糖和食物连锁分析)与多种组学测量一起用于饮食摄入量的生物标志物预测,因为这项研究具有重要意义和影响力。高分辨率食品聚糖库以及这些食品的聚糖微生物生物标志物产品将改变未来以饮食为重要组成部分的临床试验。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
- DOI:10.1021/jasms.1c00133
- 发表时间:2021-08-04
- 期刊:
- 影响因子:3.2
- 作者:Castillo JJ;Galermo AG;Amicucci MJ;Nandita E;Couture G;Bacalzo N;Chen Y;Lebrilla CB
- 通讯作者:Lebrilla CB
The Development of the Davis Food Glycopedia-A Glycan Encyclopedia of Food.
- DOI:10.3390/nu14081639
- 发表时间:2022-04-14
- 期刊:
- 影响因子:5.9
- 作者:
- 通讯作者:
Quantitative Bottom-Up Glycomic Analysis of Polysaccharides in Food Matrices Using Liquid Chromatography-Tandem Mass Spectrometry.
- DOI:10.1021/acs.analchem.2c03707
- 发表时间:2023-01-17
- 期刊:
- 影响因子:7.4
- 作者:Bacalzo, Nikita P.;Couture, Garret;Chen, Ye;Castillo, Juan J.;Phillips, Katherine M.;Fukagawa, Naomi K.;Lebrilla, Carlito B.
- 通讯作者:Lebrilla, Carlito B.
Associations of Human Milk Oligosaccharides and Bioactive Proteins with Infant Morbidity and Inflammation in Malawian Mother-Infant Dyads.
- DOI:10.1093/cdn/nzab072
- 发表时间:2021-05
- 期刊:
- 影响因子:4.8
- 作者:Jorgensen JM;Young R;Ashorn P;Ashorn U;Chaima D;Davis JCC;Goonatilleke E;Kumwenda C;Lebrilla CB;Maleta K;Sadalaki J;Totten SM;Wu LD;Zivkovic AM;Dewey KG
- 通讯作者:Dewey KG
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CARLITO B LEBRILLA其他文献
CARLITO B LEBRILLA的其他文献
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{{ truncateString('CARLITO B LEBRILLA', 18)}}的其他基金
Annotating carbohydrate structures to develop markers for consumption of food
注释碳水化合物结构以开发食物消耗标记
- 批准号:
10133066 - 财政年份:2020
- 资助金额:
$ 60.61万 - 项目类别:
Annotating carbohydrate structures to develop markers for consumption of food
注释碳水化合物结构以开发食物消耗标记
- 批准号:
10312118 - 财政年份:2020
- 资助金额:
$ 60.61万 - 项目类别:
Glycomics Equipment: NanoLC CHIP-QTOF Mass Spectrometer
糖组学设备:NanoLC CHIP-QTOF 质谱仪
- 批准号:
7794794 - 财政年份:2010
- 资助金额:
$ 60.61万 - 项目类别:
The development of mass spectrometry for Clinical Glycomics
临床糖组学质谱分析的发展
- 批准号:
7935886 - 财政年份:2009
- 资助金额:
$ 60.61万 - 项目类别:
Structures, Functions, & Genetic Diversity of Oligosaccharides in Milk
结构、功能、
- 批准号:
8487419 - 财政年份:2009
- 资助金额:
$ 60.61万 - 项目类别:
Structures, Functions, & Genetic Diversity of Oligosaccharides in Milk
结构、功能、
- 批准号:
8305556 - 财政年份:2009
- 资助金额:
$ 60.61万 - 项目类别:
Structures, Functions, & Genetic Diversity of Oligosaccharides in Milk
结构、功能、
- 批准号:
7739537 - 财政年份:2009
- 资助金额:
$ 60.61万 - 项目类别:
Structures, Functions, & Genetic Diversity of Oligosaccharides in Milk
结构、功能、
- 批准号:
8096559 - 财政年份:2009
- 资助金额:
$ 60.61万 - 项目类别:
Structures, Functions, & Genetic Diversity of Oligosaccharides in Milk
结构、功能、
- 批准号:
7931910 - 财政年份:2009
- 资助金额:
$ 60.61万 - 项目类别:
Necrotizing Enterocolitis: Prevention and Prediction
坏死性小肠结肠炎:预防和预测
- 批准号:
7933176 - 财政年份:2009
- 资助金额:
$ 60.61万 - 项目类别:
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