Nanoporous silicon membranes for protein purification

用于蛋白质纯化的纳米多孔硅膜

• 批准号: 7802530
• 负责人: THOMAS R GABORSKI
• 金额: $ 15.32万
• 依托单位: SIMPORE, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7802530
• 关键词: Albumins; Area; Benchmarking; Capillary Electrophoresis; Characteristics; Charge; Chromatography; Column Chromatography; Complex; Core Facility; Defect; Deposition; Devices; Drops; Equipment; Excision; Failure; Film; Filtration; Fractionation; Funding; Goals; Grant; Immunoglobulin G; Industry; Laboratories; Liquid Chromatography; Membrane; Membrane Proteins; Molecular Weight; Permeability; Phase; Procedures; Production; Proteins; Research; Research Project Grants; Running; Sampling; Series; Silicon; Speed; Surface; System; Techniques; Technology; Testing; Thick; Thinness; Time; Tube; United States National Institutes of Health; Universities; Work; base; biomedical scientist; cytochrome c; density; nano; nanocrystal; nanometer; particle; phase 2 study; pressure; protein complex; protein purification; public health relevance; research study; response; tool; two-dimensional

DESCRIPTION (provided by applicant): In this Phase I application we will examine the feasibility of producing high yield porous nanocrystalline silicon (pnc-Si) membranes for protein purification products. Membranes made of this material are a breakthrough technology, 1000x thinner than conventional and other nanoporous membranes, with tunable pore sizes (5-100 nm) and precise distributions. At just tens of nanometers thick, pnc-Si membranes have no internal void spaces, resulting in minimal loss of filtrate and high permeability, making them ideally suited for rapid and precise biomolecule separations. Research of cellular components and proteins has risen to new levels with advances in separation tools such as two-dimensional liquid chromatography and capillary electrophoresis systems. These tools, however, are typically limited to core facilities at major research centers away from the bench top or the daily workspace of the average biomedical scientist. Laboratory separation and sample- prep tools haven't kept pace with advances in major core facilities. This is in part due to the fact that basic membrane technology has not significantly advanced in decades, still suited best to concentration or micron-scale separations. Pnc-Si membranes will enable consumable separation products that bridge the gap between bench top tools and core facility equipment. In this project we will test the feasibility of scalable production of pnc-Si membranes at reproducible pore sizes with adequate burst pressures to be integrated into stackable membrane modules. We will perform protein concentration and purification experiment using pnc-Si membranes in series and benchmark against industry standard centrifugal spin-tubes. Phase II efforts will fabricate stackable modules with precise cut-offs that fractionate and concentration complex protein mixtures in one pass with the goal of offering chromatography features with the speed and simplicity of membrane filtration. PUBLIC HEALTH RELEVANCE: SiMPore's pnc-Si membranes are a breakthrough technology 1000x thinner than conventional and other nanoporous membranes, with permeability more than 100 times greater and controllable tight pore size distributions. These characteristics enable protein separation and purification with the precision of liquid chromatography, but the speed and simplicity of a membrane filter.

描述（由申请人提供）：在此I阶段申请中，我们将检查产生高屈服多孔纳米晶硅（PNC-SI）膜的可行性，用于蛋白质纯化产物。由这种材料制成的膜是一种突破性的技术，比传统和其他纳米孔薄1000倍，具有可调孔尺寸（5-100 nm）和精确的分布。在仅数十纳米厚的纳米时，PNC-SI膜没有内部空间空间，导致滤液的损失最小和高渗透性，使其非常适合快速，精确的生物分子分离。通过分离工具（例如二维液相色谱和毛细管电泳系统）的进​​步，对细胞成分和蛋白质的研究已提高到新水平。但是，这些工具通常仅限于主要研究中心的核心设施，远离台式上的台式或普通生物医学科学家的日常工作空间。实验室分离和样品准备工具尚未与主要核心设施的进步保持同步。这部分是由于以下事实：基本的膜技术在几十年中没有显着发展，但仍然最适合集中度或微米级分离。 PNC-SI膜将启用可消耗的分离产品，以弥合台式工具和核心设备设备之间的差距。在这个项目中，我们将测试可重复的孔径可扩展PNC-SI膜的可行性，并具有足够的爆发压力，可以集成到可堆叠的膜模块中。我们将使用PNC-SI膜进行蛋白质浓度和纯化实验，并针对行业标准离心管进行基准。第二阶段的工作将使用精确的截止量制造可堆叠的模块，这些模块在一个通道中分解和浓度复杂的蛋白质混合物，以提供具有膜过滤速度和简单性的色谱特征。 公共卫生相关性：Simpore的PNC-SI膜是一种突破性的技术，比传统和其他纳米方膜薄1000倍，渗透性高100倍，可控制的紧密孔径分布。这些特征可以通过液相色谱的精度来蛋白质分离和纯化，但膜过滤器的速度和简单性。