A Rapid Label-free Sensor for Immune Markers of Environmental Exposure

一种快速无标记传感器，用于检测环境暴露的免疫标记物

基本信息

批准号：
7363511
负责人：
Christopher Striemer
金额：
$ 19.2万
依托单位：
PATHOLOGICS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-24 至 2009-09-09
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7363511
关键词：
Acute respiratory infection Address Affect Airborne Particulate Matter Albumins Amines Animal Model Animals Antibodies Benchmarking Binding Binding Sites Biological Biological Assay Biosensing Techniques Blood Blood specimen Bovine Serum Albumin Buffers Cardiovascular Diseases Chemicals Chemistry Chronic Computers Cosmetics Data Detection Development Devices Disease Elements Environmental Exposure Enzyme-Linked Immunosorbent Assay Esters Event Exposure to Fingers Fire - disasters Funding Opportunities Gastrointestinal tract structure Glutaral Goals Growth Health Hour Human Human Activities Image Immune system Immunologic Markers Individual Interferons Label Literature MYO5A gene Malignant neoplasm of lung Measurement Measures Methods Molecular Monoclonal Antibodies Mucous body substance Nanotechnology Operative Surgical Procedures Paint Particulate Matter Pathologic Performance Phase Physiological Process Protein Microchips Proteins Protocols documentation Range Reader Relative (related person)Research Research Design Risk Saliva Sampling Sensitivity and Specificity Serum Signaling Protein Silicon Dioxide Site Skin Solutions Source Specificity Staging Standards of Weights and Measures Structure Sunscreening Agents Surface Techniques Technology Testing Time Toxic effect Triton X100 Tweens Ultrafine United States Environmental Protection Agency Universities Urine Validation antigen antibody binding base biochip commercial application concept consumer product cross reactivity density design environmental agent experience forest improved instrument laptop prototype respiratory response sensor size success surfactant urban area

Acute respiratory infection Address Affect Airborne Particulate Matter Albumins Amines Animal Model Animals Antibodies Benchmarking Binding Binding Sites Biological Biological Assay Biosensing Techniques Blood Blood specimen Bovine Serum Albumin Buffers Cardiovascular Diseases Chemicals Chemistry Chronic Computers Cosmetics Data Detection Development Devices Disease Elements Environmental Exposure Enzyme-Linked Immunosorbent Assay Esters Event Exposure to Fingers Fire - disasters Funding Opportunities Gastrointestinal tract structure Glutaral Goals Growth Health Hour Human Human Activities Image Immune system Immunologic Markers Individual Interferons Label Literature MYO5A gene Malignant neoplasm of lung Measurement Measures Methods Molecular Monoclonal Antibodies Mucous body substance Nanotechnology Operative Surgical Procedures Paint Particulate Matter Pathologic Performance Phase Physiological Process Protein Microchips Proteins Protocols documentation Range Reader Relative (related person)Research Research Design Risk Saliva Sampling Sensitivity and Specificity Serum Signaling Protein Silicon Dioxide Site Skin Solutions Source Specificity Staging Standards of Weights and Measures Structure Sunscreening Agents Surface Techniques Technology Testing Time Toxic effect Triton X100 Tweens Ultrafine United States Environmental Protection Agency Universities Urine Validation antigen antibody binding base biochip commercial application concept consumer product cross reactivity density design environmental agent experience forest improved instrument laptop prototype respiratory response sensor size success surfactant urban area

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项目摘要

DESCRIPTION (provided by applicant) Airborne particulate matter (PM) derived from a variety of natural sources (volcanic activity, forest fires) is omnipresent. However, human activity since the industrial revolution and the advent of the internal combustion engine has substantially increased the concentration of PM, particularly in urban areas. Human derived PM tends to be of smaller size, facilitating entry into the body through the respiratory and digestive tracts, as well as through the skin. It also tends to have adsorbed chemical species with greater toxicity than its natural counterparts. The explosive growth of nanotechnology in research and consumer products (sunscreens, cosmetics, paint) is also creating an array of ultrafine materials with largely unknown health effects. Despite numerous studies implicating PM in various disorders including acute respiratory infections, lung cancer, and chronic respiratory and cardiovascular diseases, the specific biological mechanisms involved are not fully understood. Pathologics seeks to leverage its proprietary Arrayed Imaging Reflectometery (AIR) biosensing platform to build a device capable of capturing the detailed physiological response to PM exposure by profiling key marker proteins in conveniently available biosamples such as a finger pick of blood, urine, mucous, saliva, etc. Currently, such testing takes hours and is often done with individual tests. AIR is capable of sensitive and simultaneous protein detection with a device that is currently the size of a laptop computer. The low complexity of the testing platform will also enable the development of handheld units that would be particularly useful for profiling exposure response in the field. In addition to saving time and offering greater convenience these multiplex sensors will allow far more molecular data to be collected than is currently feasible.

描述（由申请人提供）来自多种自然来源（火山活动，森林火灾）的空气颗粒物（PM）无处不在。但是，自工业革命和内燃机出现以来的人类活动大大提高了PM的集中度，尤其是在城市地区。人类衍生的PM往往具有较小的大小，通过呼吸道和消化道以及通过皮肤促进进入体内的PM。它还倾向于具有比其天然对应物更大的毒性吸附的化学物种。纳米技术在研究和消费产品中的爆炸性增长（防晒霜，化妆品，油漆）也正在创造一系列具有未知健康影响的超铁材料。尽管许多研究涉及PM在各种疾病中，包括急性呼吸道感染，肺癌以及慢性呼吸道和心血管疾病，但涉及的特定生物学机制尚未完全了解。病理学旨在利用其专有的阵列成像反射仪（AIR）生物传感平台，以构建一种能够通过在便利的生物样本中分析钥匙标记蛋白来捕获对PM暴露的详细生理反应的设备，例如血液，尿液，粘液，粘液，唾液等，当前进行测试，经常进行单个测试。空气能够使用目前与笔记本电脑大小的设备进行敏感且同时的蛋白质检测。测试平台的低复杂性还将使手持单元的开发对于对现场的分析暴露响应特别有用。除了节省时间并提供更大的便利性，这些多重传感器将允许收集比目前可行的更多分子数据。