Transparent Ultrathin Nanomembranes for Barrier Cell Models and Novel Co-Culture Systems

用于屏障细胞模型和新型共培养系统的透明超薄纳米膜

基本信息

批准号：
9336323
负责人：
THOMAS R GABORSKI
金额：
$ 35.66万
依托单位：
ROCHESTER INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2021-05-31
项目状态：
已结题

项目摘要

Abstract The main goals of this research program are to; one, research and develop transparent ultrathin nanomembranes and two, utilize these membranes to advance biomedical in vitro model systems through work in the laboratory of the PI and current and future collaborators. Nanomembrane development will include research toward fabricating ultrathin nanoporous silicon dioxide membranes, scaling up their active area, creating unique surface chemistries to promote cellular interaction and integration of sensor technologies. This research program will enable collaborators to visualize endothelial barrier transmigration, produce better in vitro corneal models and visualize motile cilia in a patient derived primary lung model of cystic fibrosis. In addition, nanomembrane development will enable and supply collaborating Investigators with the tools to solve existing challenges and expand their respective fields. A common need is the ability to culture cells in a physiologically relevant model system that can be visualized in real-time. Transparent ultrathin porous membranes can accomplish this for almost any barrier model and co-culture system. SiO2 nanomembranes enable co-cultured cells to be brought within physiological separations distances (~100 nm), while providing glass-like optical transparency and nearly unhindered transport of signaling molecules. Success in developing new human in vitro systems promises to reduce the reliance on animal models, while simultaneously increasing physiological relevance and accelerating drug development. These tissue- and organ-on-a-chips also make feasible live imaging of complex cellular events that require sophisticated and well-orchestrated microenvironments.

抽象的该研究计划的主要目标是：一个，研究和发展透明的超薄纳米膜和两个，利用这些膜来推进生物医学的体外模型通过在PI和当前和未来合作者实验室工作的工作。纳米膜开发将包括用于制造超薄纳米方的研究二氧化硅膜，扩大其活性区域，创建独特的表面化学成分促进传感器技术的细胞相互作用和整合。该研究计划将使合作者能够可视化内皮屏障的迁移，在体外产生更好的体外角膜模型，并在患者衍生的囊性原发性肺模型中可视化纤毛纤维化。此外，纳米膜开发将启用和供应合作调查人员使用解决现有挑战并扩大各自领域的工具。一个共同的需求是能够在生理相关模型系统中培养细胞的能力实时可视化。透明的超薄多孔膜可以为此做到这一点几乎所有障碍模型和共同文化系统。 SiO2纳米膜可实现共培养细胞在提供类似玻璃的同时，将其带入生理分离距离（〜100 nm）光学透明度和信号分子的几乎不受阻碍的运输。成功开发新的人类体外系统有望减少对动物模型的依赖，同时增加了生理相关性并加速了药物开发。这些组织和器官片也使复杂细胞事件的可行实时成像可行这需要精致且经过精心策划的微环境。