Admin Core

管理核心

基本信息

批准号：
7784476
负责人：
HOWARD S FOX
金额：
$ 70.8万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-03-09 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7784476
关键词：
AIDS neuropathy Achievement Activity Cycles Anatomy Animal Model Applications Grants Area Arts Auditory Evoked Potentials Benchmarking Biological Assay Biological Models Brain Brain region Calcium Canada Cell Culture Techniques Cells Circadian Rhythms Communities Detection Disease Disease Progression Elements Engineering Environment Evaluation Event Evoked Potentials Faculty Family Felidae Feline Immunodeficiency Virus France Functional disorder Funding Future Genetic Goals HIV-1 Host Defense Host Defense Mechanism Human Image Imagery Immune Immune response In Vitro Infection Institutes Institution International Knowledge Life Manuscripts Measurement Measures Membrane Methodology Methods Mexico Microscopic Modeling Molecular Monkeys Mus Nervous System Physiology Netherlands Neuraxis Neurons Online Systems Pathogenesis Pathology Phenotype Physiological Physiology Preparation Primates Process Property Radio Rattus Recruitment Activity Research Research Personnel Rodent SIV Sensory Services Sleep Slice Slovakia Spectrum Analysis Stream Study models Subfamily lentivirinae Switzerland Synapses Techniques Telemetry Temperature Therapeutic Training Viral Virus Visual Work base chemokine clinical phenotype cytokine defense response extracellular in vitro Assay in vitro Model in vivo innovation interest meetings mouse model neural circuit neuropathology neurophysiology new technology novel strategies patch clamp preclinical study programs receptor receptor expression success

项目摘要

The major goal of the Physiology Core of the Scripps NeuroAIDS Preclinical Studies (SNAPS), CSPAR, is to continue to provide neurophysiological assessment of AIDS-related functional pathology using a variety of established and new animal models with clinical phenotypes relevant for neuroAIDS. The Core services will include continued evaluation of current primate and feline models infected with the analogous lentivirus (SIV and FIV, respectively) as well as assessment of existing and new mouse models representing molecularly engineered phenotypes relevant for HIV-1 infection. The Core will provide a broad spectrum of analyses for the detection of functional disease progression and identification of underlying mechanisms. In addition, the Core will work to recruit new SNAPS collaborators who will further enrich the assessment potential of the Core and its contribution to the neuroAIDS field. The Core is organized into two interacting units that carry out specific scientific analyses. The analyses provide sensitive measures of disease progression, functional deficits and underlying mechanisms. The in vivo analysis unit will carry out studies in mouse, primate and feline models of NeuroAIDS utilizing in vivo techniques including extracellular single unit electrophysiological recording, sensory potential recording and radio telemetry. The in vitro analysis unit will use electrophysiological recordings of brain slices from animal models of NeuroAIDS or cultures prepared from these models. The in vitro unit will also carry out studies of these models using live cell measurement of cytosolic calcium levels and anatomical studies of receptor expression and localization. Important strengths of this SNAPS Core include the expertise and knowledge of the faculty and staff, their long-term working relationships, and their commitment to neuroAIDS research.

斯克里普斯神经艾滋病临床前研究 (SNAPS) 生理学核心 (CSPAR) 的主要目标是继续使用各种方法提供艾滋病相关功能病理学的神经生理学评估建立了具有与神经艾滋病相关的临床表型的新动物模型。核心服务将包括继续评估当前感染类似慢病毒（SIV）的灵长类动物和猫科动物模型和 FIV 分别）以及对代表分子水平的现有和新小鼠模型的评估与 HIV-1 感染相关的工程表型。核心将为以下方面提供广泛的分析：检测功能性疾病进展并识别潜在机制。此外， Core 将努力招募新的 SNAPS 合作者，他们将进一步丰富 SNAPS 的评估潜力核心及其对神经艾滋病领域的贡献。核心被组织成两个相互作用的单元，做出具体的科学分析。这些分析提供了疾病进展、功能性的敏感测量缺陷和潜在机制。体内分析单位将在小鼠、灵长类动物和利用体内技术（包括细胞外单单元电生理学）的 NeuroAIDS 猫科动物模型记录、感觉电位记录和无线电遥测。体外分析装置将使用 NeuroAIDS 动物模型或由以下物质制备的培养物脑切片的电生理记录这些模型。体外单位还将利用活细胞测量对这些模型进行研究胞质钙水平以及受体表达和定位的解剖学研究。重要优势 SNAPS 核心的核心包括教职员工的专业知识和知识、他们的长期工作关系，以及他们对神经艾滋病研究的承诺。