Shared Resources: Functional Genomics

共享资源：功能基因组学

• 批准号: 7944596
• 负责人: TONY R. HUNTER
• 金额: $ 21.32万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-14 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944596
• 关键词: Base Sequence; Biodistribution; Cancer Center Support Grant; Communication; Complementary DNA; Computer software; Core Facility; DNA; Data; Databases; Detection; Discrimination; Equipment; Funding; Gene Chips; Gene Expression; Gene Expression Profiling; Generations; Genes; Genome; Genotype; Hour; Human; Image; Institutes; Label; Liquid substance; Molecular Biology; Mus; Nucleic acid sequencing; Oligonucleotides; Operating System; Preparation; RNA; Rana catesbeiana; Research Personnel; Resolution; Resource Sharing; Robot; Robotics; Running; SNP genotyping; Scanning; Services; Slide; Small Interfering RNA; Source; Spottings; Staining method; Stains; System; Time; Training; Transgenic Mice; Validation; Vision; cDNA Arrays; data acquisition; density; functional genomics; gene therapy; instrument; meetings; microbial; molecular dynamics; plasmid DNA; programs

The Functional Genomics Facility provides cDNA microarrays, Affymetrix oligo arrays, and computational and experimental support for investigators conducting studies of gene expression. The Facility also provides gene expression analysis using real time PCR. The real time PCR service is helpful to investigators conducting studies of gene expression profiling, copy number determination of DNA or RNA targets, siRNA knockdown validation, allelic discrimination/SNP genotyping, transgenic mouse genotyping, biodistribution studies of gene therapy products, and microbial detection.

功能基因组学设施提供 cDNA 微阵列、Affymetrix 寡核苷酸阵列和计算 为研究人员进行基因表达研究提供实验支持。该设施还提供 使用实时 PCR 进行基因表达分析。实时PCR服务对研究人员有帮助 进行基因表达谱研究、DNA 或 RNA 靶标拷贝数测定、siRNA 敲低验证、等位基因辨别/SNP 基因分型、转基因小鼠基因分型、生物分布 基因治疗产品和微生物检测的研究。