CYST WALL ENDOPROTEASES OF ENTAMOEBA INVADENS AND ENTAMOEBA HISTOLYTICA

入侵内阿米巴和溶组织内阿米巴的囊壁内切蛋白酶

• 批准号: 7955937
• 负责人: John C. Samuelson
• 金额: $ 0.47万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955937
• 关键词: Alpha-mannosidase; Amebic colitis; Anabolism; Anti-Retroviral Agents; Biochemical; Biology; Complex; Computer Retrieval of Information on Scientific Projects Database; Concanavalin A; Cyanovirin-N; Cyst; Endoplasmic Reticulum; Entamoeba histolytica; Entamoeba invadens; Funding; Glucosyltransferase; Glucosyltransferases; Glycoproteins; Grant; Institution; Lectin; Link; Liver Abscess; Mass Spectrum Analysis; Medicine; Methods; Polysaccharides; Quality Control; Research; Research Personnel; Resources; Source; Surface; System; United States National Institutes of Health; Upper arm; interest; protein folding; sugar nucleotide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. N-Glycans of Entamoeba histolytica, the protist that causes amebic dysentery and liver abscess, are of great interest for multiple reasons. E. histolytica makes an unusual truncated N-glycan precursor (Man(5)GlcNAc(2)), has few nucleotide sugar transporters, and has a surface that is capped by the lectin concanavalin A. Here, biochemical and mass spectrometric methods were used to examine N-glycan biosynthesis and the final N-glycans of E. histolytica with the following conclusions. Unprocessed Man(5)GlcNAc(2), which is the most abundant E. histolytica N-glycan, is aggregated into caps on the surface of E. histolytica by the N-glycan-specific, anti-retroviral lectin cyanovirin-N. Glc(1)Man(5)GlcNAc(2), which is made by a UDP-Glc: glycoprotein glucosyltransferase that is part of a conserved N-glycan-dependent endoplasmic reticulum quality control system for protein folding, is also present in mature N-glycans. A swainsonine-sensitive alpha-mannosidase trims some N-glycans to biantennary Man(3)GlcNAc(2). Complex N-glycans of E. histolytica are made by the addition of alpha1,2-linked Gal to both arms of small oligomannose glycans, and Gal residues are capped by one or more Glc. In summary, E. histolytica N-glycans include unprocessed Man(5)GlcNAc(2), which is a target for cyanovirin-N, as well as unique, complex N-glycans containing Gal and Glc.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 由于多种原因，引起阿米比克痢疾和肝脓肿的原生物学家的n-glycans是溶血症的N-聚糖。 E. histolytica makes an unusual truncated N-glycan precursor (Man(5)GlcNAc(2)), has few nucleotide sugar transporters, and has a surface that is capped by the lectin concanavalin A. Here, biochemical and mass spectrometric methods were used to examine N-glycan biosynthesis and the final N-glycans of E. histolytica with the following conclusions.未经处理的人（5）GlcNAC（2）是最丰富的E. histoltica n-Glycan，由N-聚糖特异性的抗逆转录病毒凝集素氰基硫醇-N聚合成蛋白酶溶液性的帽。 GLC（1）MAN（5）GLCNAC（2）是由UDP-GLC制成的：蛋白质折叠的蛋白质折叠的保守的N-聚糖依赖性内质质量控制系统的一部分，它是成熟的N-聚合物中的蛋白质折叠的一部分。瑞士宁敏感的α-甘露糖苷酶将一些N-聚糖丝（3）glcNAC（2）修剪。溶组织溶液性的复杂N-聚糖是通过将α1,2连接的GAL添加到小寡素糖甘氨酸的两个臂中，而GAL残基由一个或多个GLC覆盖。总而言之，溶血性的n-glycans包括未经加工的男子（5）GlcNAC（2），这是氰基维林-N的靶标，以及含有GAL和GLC的独特，复杂的N-Glycans。