CYST WALL ENDOPROTEASES OF ENTAMOEBA INVADENS AND ENTAMOEBA HISTOLYTICA

入侵内阿米巴和溶组织内阿米巴的囊壁内切蛋白酶

• 批准号: 7602035
• 负责人: John C. Samuelson
• 金额: $ 0.86万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602035
• 关键词: Affect; Affinity; Cell Extracts; Computer Retrieval of Information on Scientific Projects Database; Cyst; Cysteine Protease; Endopeptidases; Entamoeba; Entamoeba histolytica; Entamoeba invadens; Event; Fluorescein-5-isothiocyanate; Funding; Genome; Grant; Homologous Gene; Institution; Label; Lectin; Maintenance; Peptide Hydrolases; Peptides; Protease Inhibitor; Proteins; Proteomics; Reptiles; Research; Research Personnel; Resources; Site; Source; Taxon; United States National Institutes of Health; enteric pathogen; inhibitor/antagonist; man; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Entamoeba invadens and Entamoeba histolytica are related enteric pathogens. The former is a pathogen affecting several reptile taxons and the later is a pathogen in man. Jacob proteins are abundant components of the cyst wall in E. invadens and homologues of it exist in E. histolytica. The Jacob proteins have conserved lectin-like domains and are likely important for maintenance of cyst wall integrity in these pathogens. Putative endoprotease sites are conserved in their sequence across Entamoeba species and therefore a proteolytic event may be important for proper function of the cyst wall. That most proteases in the E. histolytica genome are cysteine proteases suggests that the putative Jacob proteases are of this type. FITC label peptides containing the putative Entamoeba protease sites have been constructed for use in strategies to detect protease activity using approaches including MALDI-TOF mass spectrometric analysis. This study attempts to identify specific protease activities in E. histolytica cell extracts in strategies using known protease inhibitors. As results indicate, specific inhibitors will be used for affinity enrichment of the proteases and proteomics approaches will be used to identify the active protease(s).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 Entamoeba Invadens和Entamoeba Histoltica是相关的肠道病原体。 前者是影响几个爬行动物分类群的病原体，后来是人的病原体。 雅各布蛋白是E. Invadens中囊肿壁的丰富成分，其同源物存在于E. histolytica中。 雅各布蛋白具有保守的凝集素样结构域，对于在这些病原体中维持囊肿壁完整性可能很重要。 假定的内蛋白酶位点在整个肠道种类的序列中是保守的，因此蛋白水解事件对于囊肿壁的正常功能可能很重要。大多数蛋白酶基因组中的大多数蛋白酶是半胱氨酸蛋白酶，表明假定的雅各布蛋白酶是这种类型的。 FITC标记肽含有推定的Entamoeba蛋白酶位点已被构建，用于在策略中使用包括MALDI-TOF质谱分析在内的方法来检测蛋白酶活性。这项研究试图在使用已知蛋白酶抑制剂的策略中鉴定E. histolictica细胞提取物中的特定蛋白酶活性。 结果表明，将使用特异性抑制剂来富集蛋白酶，蛋白质组学方法将用于识别活性蛋白酶。