LIFTETIME MEASUREMENTS OF CRYPTOPHANE-XENON COMPLEXES

隐烷-氙复合物的寿命测量

• 批准号: 7955447
• 负责人: Ivan Julian Dmochowski
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955447
• 关键词: Affinity; Binding; Biosensor; Chemicals; Complex; Computer Retrieval of Information on Scientific Projects Database; Fluorescence; Funding; Goals; Grant; Institution; Laboratories; Magnetic Resonance Imaging; Measurement; Measures; Nature; Optics; Process; Relaxation; Research; Research Personnel; Resources; Screening for cancer; Solutions; Source; Therapeutic Intervention; Time; United States National Institutes of Health; Xenon; aqueous; chromophore; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of our overall project is to develop 129-Xe MRI biosensors for early cancer detection and possible therapeutic intervention. We are currently synthesizing cryptophanes and other carriers that will have greater affinity for xenon, longer 129Xe hyperpolarized spin-lattice relaxation times, and unique chemical shifts. Cryptophane-A, an organic cage compound, binds xenon (Xe) in its internal cavity with the highest affinity of any known molecule and fluoresces with a maximum intensity around 314nm. The presence of excess Xe in aqueous solution with the cryptophane quenches the observed fluorescence with an efficiency of about 50% as measured by steady-state experiments. This quenching is believed to be largely static in nature, a result of the bound Xe atom residing very close to the veratole chromophores of the cryptophane. Fluorescence lifetime measurements would allow for the determination of the biomolecular quenching constant or a confirmation of the static quenching process.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们整体项目的目的是开发129-XE MRI生物传感器，用于早期癌症检测和可能的治疗干预。我们目前正在合成对氙气，更长的129倍超极化旋转晶格松弛时间和独特的化学移位的隐胎和其他载体。一种有机笼化合物的隐烷-a结合其内部腔中的氙（XE），其具有最高的任何已知分子和荧光的亲和力，最大强度约为314nm。通过稳态实验测量的效率约为50％，在水溶液中存在过量的Xe在水溶液中的存在。这种淬火本质上被认为是很大程度上静态的，这是由于结合的Xe原子非常靠近隐形烷的veratole发色团的结果。荧光寿命测量将允许确定生物分子淬灭常数或确认静态淬火过程。