HUMAN BRAIN ANTIOXIDANT LEVELS MEASURED IN VIVO AS A FUNCTION OF AGE AND DIET

体内测量的人脑抗氧化水平与年龄和饮食的关系

• 批准号: 7951744
• 负责人: Melissa J Terpstra
• 金额: $ 0.62万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7951744
• 关键词: Accountability; Age; Aging; Antioxidants; Appearance; Ascorbic Acid; Biological Assay; Blood; Brain; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; Controlled Study; Development; Diagnosis; Diet; Elderly; Enrollment; Etiology; Funding; Grant; Habits; Human; Institution; Intervention; Label; Magnetic Resonance Spectroscopy; Measurement; Measures; Monitor; Neuraxis; Neurodegenerative Disorders; Nursing Staff; Oxidative Stress; Participant; Population; Reduced Glutathione; Research; Research Personnel; Resources; Role; Scanning; Serum; Source; Supplementation; Symptoms; Therapeutic Intervention; United States National Institutes of Health; base; cohort; dietary control; fruits and vegetables; in vivo; inclusion criteria; normal aging; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although oxidative stress is known to be involved early in the etiologies of both normal aging and neurodegenerative disease, lack of a noninvasive assay for brain oxidative status has limited development of antioxidant based interventions. The hypotheses of this proposal is that magnetic resonance spectroscopy (MRS) can provide a noninvasive assay for human brain antioxidant concentrations that can be used to: identify influences responsible for compromised antioxidant levels, diagnose reduced antioxidant capacity prior to appearance of symptoms or structural damage, and monitor antioxidant capacity throughout therapeutic intervention. In part 1 of this study, a novel noninvasive MRS assay will be used to quantify levels of the two most concetnrated non-enzymatic antioxidants in the central nervous system, reduced glutathione (GSH) and vitamin C (Asc) in two homogenous human populations, a young and an elder cohort. In the second part, this antioxidant profile will be measured under the respective endogenous and exogenous influences of aging and diet. The role of the GCRC will be to assist in the implementation of appropriate dietary control for this study. The key role of the GCRC in this study will be to assess deitary habits and to provide a vitamin C controlled diet for three days prior to each MR exam. The GCRC will also evaluate fruit and vegetable screeners so that only cadidates who consume fewer than five fruits and vegetables per day are enrolled. This inclusion criteria was selected in order to optimize feasibility of measuring a difference in pre- versus post- vitamin C supplementation. The GCRC will also council participants regarding the need to refrain from intentional and incidental vitamin C supplementation. In order to potentially correlate brain and serum Asc levels, the nursing staff from the GCRC will collect blood for measurement of serum vitamin C concentrations complimentary to each MR scan. The GCRC will also label (attached), store, dispense, and keep an accountability log (attached) for vitamin C supplements which will be provided to elder participants.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 尽管已知氧化应激涉及正常衰老和神经退行性疾病的病因，但缺乏针对脑氧化状态的无创测定的基于抗氧化剂的干预措施的发展有限。 该提议的假设是，磁共振光谱（MRS）可以为人脑抗氧化剂浓度提供无创测定，可用于以下方法：确定造成抗氧化剂水平的影响，诊断降低抗氧化剂的能力，在出现症状或结构性损害或抗氧化能力和抗氧化能力之前，抗氧化能力降低。 在本研究的第1部分中，一种新型的非侵入性MRS分析将用于量化中枢神经系统中两种最合并的非酶抗氧化剂的水平，在两个同质的人类种群中，谷胱甘肽（GSH）和维生素C（ASC）降低，年轻人和一个年轻的人群。 在第二部分中，这种抗氧化剂的特征将在各自的内源性和外源性影响下进行衰老和饮食的影响。 GCRC的作用将是协助为这项研究实施适当的饮食控制。 GCRC在这项研究中的关键作用将是评估神经习惯，并在每次MR检查前三天提供维生素C控制饮食。 GCRC还将评估水果和蔬菜筛选器，以便每天只需少于五种水果和蔬菜而陷入困境。 选择此纳入标准是为了优化测量维生素C补充前与维生素C的差异的可行性。 GCRC还将理会参与者，避免有意和附带的维生素C补充。 为了潜在地关联大脑和血清ASC水平，GCRC的护理人员将收集血液，以测量每次MR扫描的补充血清维生素C浓度。 GCRC还将标记（附件），商店，分配并保留维生素C补充剂的责任日志（附件），并将提供给老年参与者。