Biospecimens

生物样本

• 批准号: 7878116
• 负责人: Ahmet Dogan
• 金额: $ 26.86万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7878116
• 关键词: Bioinformatics; Biometry; Cells; Chronic Lymphocytic Leukemia; Classification; Clinic; Clinical; Clinical Data; Clinical Research; Consent; Cytogenetics; DNA; Data; Databases; Development; Diagnosis; Diagnostic; Extramural Activities; Family Study; Flow Cytometry; Fluorescent in Situ Hybridization; Freezing; Fresh Tissue; Funding; Future; Genomics; Goals; Grant; Image Analysis; Immunohistochemistry; In Situ Hybridization; Informed Consent; Institution; Iowa; Lymphoma; Molecular Epidemiology; Newly Diagnosed; Paraffin Embedding; Pathology; Patients; Plasma; Process; Protocols documentation; Quality Control; RNA; Relapse; Reproduction spores; Research; Research Ethics Committees; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Retrieval; Sampling; Serum; Services; Small-Cell Lymphoma; Source; Specialized Program of Research Excellence; Specimen; Suspension substance; Suspensions; Time; Tissue Microarray; Tissues; Translational Research; Tumor Tissue; United States National Institutes of Health; Universities; Work; Writing; career development; cell bank; digital imaging; follow-up; laser capture microdissection; peripheral blood; programs; tumor

The UI/MC SPORE Biospecimens Core provides a coordinated, centralized, and dedicated Core for the procurement, processing and annotation of biospecimens from lymphoma patients and patients with small lymphocytic lymphoma/chronic lymphocytic leukemia (SLL/CLL). The goal of the Biospecimens Core is to procure a variety of biologic specimens on all patients involved in UI/MC SPORE protocols and all newly diagnosed lymphoma patients seen at the University of Iowa and the Mayo Clinic Rochester. The specific aims of the Biospecimens Core are: 1) to collect, process, distribute, and bank cells from fresh tumor, frozen tumor tissue, paraffin-embedded tumor tissue, serum/plasma, and genomic DNAfrom lymphoma and SLL/CLL patients; 2) to track all biospecimens in the Biospecimens Tracking Database; and 3) to serve as a resource of expertise, collaborative support (including other NIH grants), and service for projects involving pathology review/classification, immunohistochemistry, in situ hybridization, fluorescent in situ hybridization, laser capture microdissection, tissue arrays, and digital image analysis. All specimens are collected and processed under tight quality control, and distributed to UI/MC SPORE researchers or banked for future SPORE research projects. These activities are tracked using a sophisticated database that merges the activities at Iowa and Mayo, and allow integration with clinical and other data collected in research projects. During the last grant cycle, the Core has consented over 1300 newly diagnosed lymphoma patients and over 350 previously diagnosed lymphoma patients for use of pathology tissues. In most of these patients we have banked serum and DNA, and on over 500 patients we have processed and banked cells from fresh tissue available after clinical work-up. The Core has supported multiple full and developmental projects, as well as Career Development awardees. The Core has also partnered with other projects that have obtained extramural funding to support epidemiologic and family studies, and has provided key infrastructure for the Molecular Epidemiology Resource. In the next grant cycle, we will continue to accrue new patient samples to the bank and work with investigators to utilize this increasingly valuable resource to support translational research projects in lymphoma.

UI/MC SPORE 生物样本核心提供了一个协调、集中和专用的核心，用于 淋巴瘤患者和患有淋巴瘤的患者的生物样本的获取、处理和注释 小淋巴细胞淋巴瘤/慢性淋巴细胞白血病（SLL/CLL）。生物样本核心的目标 是为参与 UI/MC SPORE 方案的所有患者以及所有新患者获取各种生物样本 在爱荷华大学和罗切斯特梅奥诊所就诊的确诊淋巴瘤患者。具体的 生物样本核心的目标是：1) 从新鲜肿瘤、冷冻肿瘤中收集、处理、分发和储存细胞 肿瘤组织、石蜡包埋的肿瘤组织、血清/血浆以及淋巴瘤和基因组 DNA SLL/CLL 患者； 2) 追踪生物样本追踪数据库中的所有生物样本； 3) 充当 专业知识资源、协作支持（包括其他 NIH 拨款）以及涉及项目的服务 病理学回顾/分类、免疫组织化学、原位杂交、荧光原位杂交、 激光捕获显微切割、组织阵列和数字图像分析。所有标本均已收集并 在严格的质量控制下进行处理，并分发给 UI/MC SPORE 研究人员或储存以供将来使用 孢子研究项目。这些活动是使用一个复杂的数据库来跟踪的，该数据库合并了 爱荷华州和梅奥分校的活动，并允许与研究项目中收集的临床和其他数据集成。 在上一个资助周期中，核心已同意超过 1300 名新诊断的淋巴瘤患者 350名先前诊断的淋巴瘤患者用于使用病理组织。在大多数此类患者中，我们 储存了血清和 DNA，我们对 500 多名患者的新鲜组织中的细胞进行了处理和储存 临床检查后即可使用。核心支持了多个完整的开发项目，以及 职业发展获奖者。 Core 还与其他已获得 校外资金支持流行病学和家庭研究，并为该研究提供了关键基础设施 分子流行病学资源。在下一个资助周期中，我们将继续积累新的患者样本 银行并与调查人员合作，利用这一日益宝贵的资源来支持转化 淋巴瘤研究项目。