Animal Imaging

动物成像

• 批准号: 7799204
• 负责人: Bradford Moffat
• 金额: $ 68.46万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-05 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7799204
• 关键词: Anesthesia procedures; Animals; Authorization documentation; Biological Markers; Bioluminescence; Brain Neoplasms; Budgets; Cell Line; Charge; Clinical; Clinical Trials; Computers; Contract Services; Data; Data Collection; Direct Costs; Disclosure; Equipment; Fees; Frequencies; Health; Human Resources; Image; Inpatients; Instruction; Isoflurane; Last Name; Lead; Magnetic Resonance Imaging; Malignant neoplasm of brain; Maps; Michigan; Modeling; Mus; Names; Operative Surgical Procedures; Outpatients; Patient Care; Patients; Perfusion; Postdoctoral Fellow; Preparation; Principal Investigator; Protocols documentation; Protons; Publications; Radio; Research; Research Personnel; Role; Scanning; Schedule; Security; Services; Spectrum Analysis; Spin Labels; System; Time; Travel; Treatment Efficacy; Universities; Wages; Weight; computerized data processing; cost; data acquisition; design; digital imaging; experience; human embryonic stem cell; image processing; image reconstruction; meetings; programs; research study; success; technique development; treatment response; tumor; water diffusion

CORE B: Animal Imaging Core. The Animal Imaging Core will perform all MRI/S and bioluminescence studies on mouse tumor models for Projects 1 and 2. Core personnel will provide these services: (a) define and implement data acquisition/imaging sequences; (b) assist with animal preparation for imaging; (c) perform all data collection on the 9.4 Tesla MRI, 7 Tesla MRI and Xenogen bioluminescence systems; (d) preliminary data processing/image reconstruction; (e) security archival of raw data and network transfer of reconstructed data to the Digital Image Processing Core (Core C); (f) scheduling of imaging experiments; and (g) provide support to Program Investigators in technical design of imaging experiments. These services designed, in part, after a "clinical MRI service" model since continual, high-volume animal scanning is essential for success of each project. More then 8000 sessions of MRI/S and 7000 bioluminescence scans will be performed over five years. Specific animal MRI/S protocols will vary with each project, but will primarily include serial studies using: standard T2-weighted; T1-weighted dynamic contrast enhanced; multislice quantitative water diffusion mapping; arterial spin labeling perfusion mapping; single-voxel proton spectroscopy. Dr. B.A. Moffat will serve as Core B Director. Dr. Moffat has extensive experience in small animal imaging research. A Co-Investigator and a Post-Doc will assist Dr. Moffat with day-to-day operations and technique development. A full time MR research technician will perform the remainder of Core B services. Pub. Health: Overall, this research effort will provide the rationale for initiation of clinical trials with combinations of molecularly targeted therapies for the treatment of malignant brain tumors. In addition, imaging biomarkers for early assessment of treatment response will be identified and validated which will lead to individualization of patient treatment. University of Michigan Ann Arbor, Michigan PHS 398(Rev. 09/04) Page 279 Form Page 2 Principal Investigator/Program Director (Last, First, Middle): ROSS, Brian D. KEY PERSONNEL. See instructions. Use continuation pages as neededto provide the required information in the format shown below. Start with Principal Investigator. List all other key personnel in alphabetical order, last name first. Name eRA Commons User Name Organization Role on Project Moffat, Bradford A. University of Michigan Core Director Schepkin, Victor University of Michigan Co-Investigator OTHER SIGNIFICANT CONTRIBUTORS Name Organization Role on Project Human Embryonic Stem Cells ¿3 NO f"l Yes If the proposed project involves human embryonic stem cells, list below the registration number of the specific cell line(s) from the following list: http://Stemcells.nih.aov/registrv/index.asp. Usecontinuation pages as needed. If a specific line cannot be referenced at this time, includea statement that one from the Registrywill be used. Cell Line Disclosure Permission Statement. Applicable to SBIR/STTROnly. SeeSBIR/STTR instructions. |~| Yes l~l No PHS 398 (Rev. 09/04) Page 280 Form Page 2-continued Number the followingpages consecutively throughout the application. Do not use suffixes such as 4a, 4b. Principal Investigator/Program Director (Last, first, middle): Ross, Brian D. FROM THROUGH DETAILED BUDGET FOR INITIAL BUDGET PERIOD DIRECT COSTS ONLY Moffat/Core B PERSONNEL (Applicant organization only) % 1 2/1 /2006 11/30/2007 DOLLAR AMOUNT REQUESTED (omit cents) TYPE EFFORT ROLE ON APPT. ON PROJECT NAME (months) PROJ. Core Moffat, Bradford Director 12 80% Co- Schepkin, Victor Investigator 12 80% Post TBN (years 1-4) Doc 12 100% Research Hoff, Benjamin (yrs 1-4) Technician 12 100% SUBTOTftl ¿ CONSULTANT COSTS EQUIPMENT (Itemize) SUPPLIES (Itemize bycategory) Cryogens $15,000 Radio Frequency Coil $3,000 Anesthesia & Isoflurane $15,750 Lab Dispensables $5,000 INST. BASE SALARY $89,610 $89,610 $55,500 $32,136 SALARY FRINGE REQUESTED BENEFITS TOTALS $71,688 $21,506 $93,194 $71 ,688 $21,506 $93,194 $55,500 $16,650 $72,150 $32,136 $9,641 $41,777 N $231,012 $69,303 $300,315 I $38,750 TRAVEL Attendance to 1-2 Scientific Meetings per Year (Post Doc & Core Director) $2,400 PATIENT CARE COSTS INPATIENT OUTPATIENT ALTERATIONS AND RENOVATIONS (Itemize bycategory) OTHER EXPENSES (Itemize bycategory) MRI 2000 Scans @ $40/Scan $80,000 Biolum Imaging (1500 scans @ $5.56) $8,340 Service Contract 9T $40,000 Computer Media $3,000 CONSORTIUM/CONTRACTUAL COSTS SUBTOTAL DIRECT COSTS FOR INITIAL BUDGET CONSORTIUM/CONTRACTUAL COSTS Bioluminescence Img. Serv. Cont. $15,000 Publication Charges $2,000 $148,340 DIRECT COSTS PEF (Item la, FacePage) $ 489,805 1 FACILITIES AND ADMINISTRATION COSTS| TOTAL DIRECT COSTS FOR INITIAL BUDGET PERIOD $ I 489,805 1 SBIR/STTR Only: FEE REQUESTED PHS 398 (Rev. 09/04) Page 281 Form Page 4

核心B：动物成像核心。动物成像芯将执行所有MRI/S和生物发光 项目1和2的小鼠肿瘤模型的研究。核心人员将提供以下服务：（a）定义 并实施数据采集/成像序列； （b）协助动物制备成像； （C） 在9.4 Tesla MRI，7 Tesla MRI和异种生物发光系统上执行所有数据收集； （D） 初步数据处理/图像重建； （e）原始数据和网络传输的安全档案 重建数据到数字图像处理核心（核心C）； （f）成像实验的调度； （g）在成像实验的技术设计方面为计划调查人员提供支持。这些 由于连续的，大量的动物扫描，因此在“临床MRI服务”模型的一部分设计之后设计的服务是在设计的。 对于每个项目的成功至关重要。超过8000次MRI/S和7000次生物发光 扫描将在五年内进行。特定的动物MRI/S协议将随着每个项目的变化，但是 主要包括串行研究，使用：标准T2加权； T1加权动态对比增强了； 多层定量水扩散映射；动脉自旋标记灌注映射；单素质子 光谱法。博士莫法特将担任B核心主管。莫法特博士在小的经验中有丰富的经验 动物成像研究。共同投资者和演出后将协助莫法特博士进行日常操作 和技术开发。全日制MR研究技术员将执行其余的核心B 服务。酒吧健康：总的来说，这项研究工作将为临床试验的主动性提供理由 与分子靶向疗法的组合用于治疗恶性脑肿瘤。此外， 将确定和验证成像生物标志物，用于早期评估治疗反应，这将 导致个性化患者治疗。 密歇根大学 密歇根州安阿伯 PHS 398（Rev. 09/04）第279页表格Page 2 首席调查员/计划主任（最后，第一，中间）：罗斯，布莱恩·D。 关键人员。请参阅说明。根据需要使用延续页面，以下面显示的格式提供所需的信息。 从首席研究员开始。按字母顺序列出所有其他关键人员，首先姓氏。 名称ERA CONSONS用户名组织在项目中的角色 莫法特，布拉德福德·A·密歇根大学核心主任 Schepkin，密歇根州维克多大学共同研究员 其他重要的贡献者 姓名组织角色 人类胚胎干细胞»3 no f“是的 如果所提出的项目涉及人类胚胎干细胞，请从以下列表中列出特定细胞系的注册数： http：//stemcells.nih.aov/registrv/index.asp。根据需要进行用户关注页。 如果目前无法引用特定行，请包括使用注册表中的一条陈述。 细胞系 披露许可声明。适用于Sbir/Stronly。 SEEESBIR/STTR说明。 |〜|是l〜l否 PHS 398（修订版09/04）第280页表格第2页。 编号以下页面不断 应用程序。请勿使用4A，4B等后缀。 首席调查员/计划主任（最后，第一，中间）：罗斯，布莱恩·D。 从通过 初始预算期的详细预算 直接费用仅莫法特/核心B 人员（仅申请人组织）％ 1 2/1/2006 11/30/2007 要求的美元金额（省略美分） 类型的努力 角色 appt。在 项目 名称（月）proj。 核 莫法特（Moffat），布拉德福德（Bradford）董事12 80％ 共同 Schepkin，Victor调查员12 80％ 邮政 TBN（1-4年）DOC 12 100％ 研究 霍夫，本杰明（YRS 1-4）技术员12 100％ subtotftl 顾问费用 设备（逐项） 供应（逐项字节） 冷冻生成$ 15,000 射频线圈$ 3,000 麻醉和异氟烷$ 15,750 实验室配件$ 5,000 研究 根据 薪水 $ 89,610 $ 89,610 $ 55,500 $ 32,136 薪水附带 要求的福利总计 $ 71,688 $ 21,506 $ 93,194 $ 71,688 $ 21,506 $ 93,194 $ 55,500 $ 16,650 $ 72,150 $ 32,136 $ 9,641 $ 41,777 n $ 231,012 $ 69,303 $ 300,315 i $ 38,750 旅行 每年参加1-2个科学会议（Post Doc＆Core董事）$ 2,400 患者护理费用住院 门诊 更改和翻新（逐项计算） 其他费用（逐项字节） MRI 2000扫描 @ $ 40/扫描$ 80,000 Biolum Imaging（1500扫描 @ $ 5.56）$ 8,340 服务合同9T $ 40,000 计算机媒体$ 3,000 财团/合同费用 初始预算的小计直接成本 财团/合同费用 生物发光IMG。服务。续。 $ 15,000 出版物收取2,000美元 $ 148,340 直接成本 PEF（la，pacepage项目） $ 489,805 1 设施和管理费用| 初始预算期间的总直接费用$ i 489,805 1 仅SBIR/STTR：要求收费 PHS 398（Rev. 09/04）第281页表格4