CORE--Animal

核心--动物

• 批准号: 7089245
• 负责人: Deborah J Clegg
• 金额: $ 26.15万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7089245
• 关键词: animal colony; bioenergetics; biomedical facility; body composition; dietary lipid; disease /disorder model; ethology; fatty acid metabolism; laboratory rat; model design /development; nuclear magnetic resonance spectroscopy; nutrition related tag; obesity; physiology; surgery; technology /technique development

The program project is aimed at understanding how diets high in fat lead to increased levels of defended body weight. Our overall strategy is to explore a model of dietary obesity at many levels and from multiple perspectives. The Animal & Physiology Core consolidates and centralizes the production of obese rats and their various control groups for each of the specific projects. Specifically, this core generates EVERY animal that is used in each of the specific projects and thereby assures that each experiment enjoys animals that have been treated in an identical manner, thus allowing comparisons across time, cohorts and projects. There are four primary goals of this core. 1) To generate requisite animals for the individual projects in any of the various dietary groups described in detail in the introductory section to the proposal. These groups include rats on a low-fat, semi-purified butter oil-based diet (LF), rats on a matched high-fat, semi-purified diet (HF), rats receiving the HF diet but with calories matched (pair-fed) to intake of the LF rats (PHF), rats receiving the LF diet and food-restricted by the same amount as the PHF: pair fed rats (PLF), and rats with pelleted chow (CHOW). The Core also generates control rats on HF and LF diets based on olive oil as opposed to butter oil. 2) To develop novel or modified rat surgical techniques as required by each of the individual projects. 3) To carry out experiments on behavioral aspects associated with the palatability and meal pattern analysis as well energy expenditure measurements to determine how the different fatty acids influence consumption and behaviors associated with body weight regulation. 4) To carry out carcass analysis for body lipid content on subjects from each of the individual projects. This includes non-invasive (i.e., NMR readings) and terminal assessments for the entire carcass or specific anatomical subareas (e.g., subcutaneous fat). Lastly, The Animal & Physiology Core will carry out certain pilot or other experiments necessary for creating data that are relevant and critical to the other projects. The Animal/Physiology Core personnel are already facile with all of the techniques required to date that are used to characterize the model; consequently the personnel are already well-trained and the protocols are all in place to efficiently accomplish the goals of this essential core.

该计划项目旨在了解高脂肪饮食如何导致脂肪水平升高 捍卫体重。我们的总体策略是探索多个层面的饮食肥胖模型 从多个角度来看。动物与生理学核心整合并集中生产 每个特定项目的肥胖大鼠及其各个对照组。具体来说，这个核心 生成每个特定项目中使用的每只动物，从而确保每个 实验喜欢以相同方式处理的动物，因此可以进行比较 跨越时间、群体和项目。该核心有四个主要目标。 1）生成必需的 各个饮食组中各个项目的动物，详细描述见 该提案的介绍部分。这些组包括服用低脂、半纯化黄油的老鼠 饮食（LF），大鼠接受匹配的高脂肪、半纯化饮食（HF），大鼠接受 HF 饮食但含有卡路里 与 LF 大鼠 (PHF) 的摄入量相匹配（配对喂养），接受 LF 饮食和食物限制的大鼠 与 PHF 相同的量：配对喂养大鼠 (PLF) 和颗粒饲料大鼠 (CHOW)。核心还 产生以橄榄油（而不是黄油）为基础的 HF 和 LF 饮食的对照大鼠。 2）开发 根据每个项目的要求，采用新颖或改良的大鼠手术技术。 3）执行 与适口性和膳食模式分析相关的行为方面的实验 能量消耗测量以确定不同脂肪酸如何影响消耗和 与体重调节相关的行为。 4) 进行胴体脂肪含量分析 关于每个单独项目的主题。这包括非侵入性（即 NMR 读数）和 对整个屠体或特定解剖分区（例如皮下脂肪）的最终评估。 最后，动物与生理学核心将进行某些试点或其他必要的实验 创建与其他项目相关且至关重要的数据。动物/生理学核心人员 已经熟悉迄今为止用于表征模型的所有技术； 因此，人员已经接受过良好培训，协议也全部到位，可以有效地 实现这一重要核心的目标。