Cell Imaging

细胞成像

• 批准号: 7030417
• 负责人: JERROLD R. TURNER
• 金额: $ 12.16万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-21 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7030417
• 关键词: bioimaging /biomedical imaging; biomedical facility; cell line; cell sorting; charge coupled device camera; electrophysiology; fluorescence recovery after photobleaching; histology; immunofluorescence technique; immunoperoxidase; microscopy; molecular /cellular imaging; time resolved data; tissue /cell culture; tissue /cell preparation; transfection

The Cell and Tissue Imaging Core is designed to provide the investigators and projects within this Program Project access to state-of-the-art techniques and equipment and the expertise to use these to their fullest capabilities. Thus, in addition to provision of equipment, services offered will include technical support, training, and assistance with experimental design, data collection, data analysis, interpretation, and preparation of presentation quality images. The core is managed by Dr. Jerrold Turner, a practicing gastrointestinal surgical pathologist who also has extensive experience with generation of transfected cell lines, quantitative and qualitative fluorescent imaging approaches, and immunostaining techniques, and is located adjacent to Dr. Turner's office and laboratory space. An experienced dedicated technician will support the operation of the core and assist investigators with all aspects of core utilization. The Specific Aims of the Cell and Tissue Imaging Core are to provide the investigators and projects within this Program Project with 1. creation of cell lines expressing fluorescent fusion proteins, including construction of appropriate expression constructs and transfection, sorting, and maintenance of cell lines, 2. time lapse imaging of live cell monolayers with simultaneous electrophysiological measurement, 3. fluorescence recovery after photobleaching (FRAP) studies, 4. immunofluorescence of cultured cells and frozen tissue sections, 5. post acquisition processing, including quantitative analysis and deconvolution, 6. histological preparation and analysis of tissues from experimental animals, and 7. immunoperoxidase staining of animal tissues. The Cell and Tissue Imaging Core facilities include two epifluorescence microscopes with heated/cooled stages, z-motors, automated electronic filter wheels, and cooled CCD cameras. A separate two-headed light microscope with high resolution color digital camera is also available. Multiple offline data analysis workstations are available within the core. The Cell and Tissue Imaging Core is supported by other core facilities at The University of Chicago, including 2 confocal microscopes and 2 inverted epifluorescence microscopes within The Integrated Microscopy Core and high speed cell sorting within the Flow Cytometry Core. SUMMARY: This core provides both routine and sophisticated microscopy approaches to Program Project Investigators. These approaches require both equipment and technical skills that are not typically available to individual laboratories. Their availability to Program Project Investigators will make it possible to complete studies that could not otherwise be accomplished and will increase both efficiency and cost-effectiveness of other studies. Thus, this core is vital to the success of the overall Program Project.

细胞和组织成像核心旨在为该计划内的研究人员和项目提供帮助 项目获得最先进的技术和设备以及充分利用这些技术和设备的专业知识 能力。因此，除了提供设备外，提供的服务还包括技术支持、 培训以及实验设计、数据收集、数据分析、解释和帮助 准备演示质量图像。核心由 Jerrold Turner 博士管理，他是一位执业医师 胃肠外科病理学家，在转染细胞的产生方面也有丰富的经验 线、定量和定性荧光成像方法以及免疫染色技术，并且是 毗邻特纳博士的办公室和实验室空间。经验丰富的专业技术人员将 支持核心的运行并协助调查人员处理核心利用的各个方面。具体 细胞和组织成像核心的目标是为该计划内的研究人员和项目提供支持 项目1.创建表达荧光融合蛋白的细胞系，包括构建 适当的表达构建体以及细胞系的转染、分选和维护，2. 延时 活细胞单层成像并同时进行电生理测量，3. 荧光 光漂白 (FRAP) 研究后的恢复，4. 培养细胞和冷冻细胞的免疫荧光 组织切片，5. 采集后处理，包括定量分析和反卷积，6. 实验动物组织的组织学制备和分析，以及7.免疫过氧化物酶 动物组织染色。细胞和组织成像核心设施包括两个落射荧光 配备加热/冷却载物台、z 电机、自动电子滤光轮和冷却 CCD 的显微镜 相机。还提供带有高分辨率彩色数码相机的独立双头光学显微镜。 核心内设有多个离线数据分析工作站。细胞和组织成像核心是 得到芝加哥大学其他核心设施的支持，包括 2 台共焦显微镜和 2 台 集成显微镜核心内的倒置落射荧光显微镜和高速细胞分选 位于流式细胞术核心内。 摘要：该核心为程序项目提供了常规和复杂的显微镜方法 调查人员。这些方法需要通常不具备的设备和技术技能 到各个实验室。他们对计划项目研究人员的可用性将使完成 原本无法完成的研究将提高效率和成本效益 其他研究。因此，这个核心对于整个计划项目的成功至关重要。