GENETIC APPROACHES TO CHOLESTROL METABOLISM IN HUMAN SUBJECT

人类受试者胆固醇代谢的遗传学方法

• 批准号: 6910658
• 负责人: Helen Haskell Hobbs
• 金额: $ 27.69万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6910658
• 关键词: bioimaging /biomedical imaging; biomedical facility; cell line; electron microscopy; fibroblasts; fluorescence microscopy; monoclonal antibody; phase contrast microscopy; tissue /cell culture

DESCRIPTION: (provided by applicant) The Tissue Culture and Microscopy Core Laboratory will be responsible for providing investigators with cultured cells, antibodies, and microscopic analysis of biological samples. The core will be under the direction of Dr.Goldstein. He will be assisted bu Drs. Y.K. Ho, Richard Anderson, Joachim Herz, and Rob Rawson. The Tissue Culture Facility of this Core is located in the Department of Molecular Genetics and consists of three suites of rooms that are used solely for tissue culture. One suite contains four work modules that open into a common work area; the second suite contains three work modules that open into a common work area; and the third area contains two work modules, one for adenovirus work and the other for baculovirus. Each module is equipped with a sterile work area (Biological Safety Cabinet hood), one or more CO2 incubators, a refrigerator, and an inverted microscope. The common work area in one suite is equipped with a stereo microscope and a phase contrast microscope, and the common work are in each of the suites contains one or two sterile work areas.

描述：（由申请人提供） 组织培养和显微镜核心实验室将负责 为研究人员提供培养细胞、抗体和显微镜 生物样品分析。核心将在 戈德斯坦博士。他将得到博士的协助。 Y.K.何，理查德·安德森，约阿希姆 赫兹和罗布·罗森。该核心的组织培养设施位于 分子遗传学系，由三间套房组成 仅用于组织培养。一套包含四个工作模块 通向公共工作区域；第二组曲包含三幅作品 打开公共工作区域的模块；第三个区域包含两个 工作模块，一个用于腺病毒工作，另一个用于杆状病毒工作。每个 模块配备无菌工作区（生物安全柜罩）， 一个或多个 CO2 培养箱、冰箱和倒置显微镜。这 一间套房的公共工作区配备了体视显微镜和相位显微镜 对比显微镜，每个套房的共同工作都包含 一两个无菌工作区。