CORE--PROTEOMICS

核心--蛋白质组学

基本信息

批准号：
7751863
负责人：
CHRISTOPH H BORCHERS
金额：
$ 30.45万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7751863
关键词：
Acetylation Address Applications Grants Arts Cancer Center Cell Cycle Regulation Cell Mobility Cell physiology Cells Complex Core Facility Development Differentiation and Growth Equipment Faculty Fluorescence Future Gel Gifts Goals Grant Human Genome Image Investments Light Maps Mass Spectrum Analysis Modems Modification Molecular Molecular Weight Names Nature Neoplasm Metastasis Non-Hodgkin&apos s Lymphoma Normal tissue morphology Paper Phosphorylation Phosphorylation Site Post-Translational Protein Processing Process Proteins Proteomics Publications Relative (related person)Research Design Research Personnel Research Support Scientist Services Site Solutions Techniques Technology Ubiquitination Work base carcinogenesis genome-wide instrumentation member method development programs protein expression research study response tumor tumorigenesis

项目摘要

The goal of the Proteomics Core is to provide mass spectrometry-based protein identification and protein characterization services to UNC researchers. These services include protein identification from gels, and solution, identification of co-immunoprecipitated proteins, and protein characterization, including mapping of phosphorylation sites. Recently, in response to the need of the researchers, the Core has implemented a new program in differential protein expression analysis, based on Amersham's DIGE technique. The Core is led by the faculty director, Dr. Christoph Borchers, the Core Director, Carol Parker, and the Assistant Director, Dr. Maria Warren. The Core adds value to the Center by supporting over twenty-one grant proposals for UNC LCCC members. Highlights of research supported by the Core include two 2003 Nature publications based on the work done in the Core: one Nature paper involved the identification of new member of the cullin complex which is involved in cell cycle regulation (for Dr. Y. Xiong); the other Nature publication involved the identification of a phosphorylation site which is critical for cellular mobility, which has implications for metastasis (for Dr. K. Jacobson). Future plans for the Core include expanding the differential protein expression program, adding to the current program in accurate molecular weight determination of intact proteins, and continuing method development for the determination of phosphorylation and ubiquitination sites.

蛋白质组学核心的目的是为UNC研究人员提供基于质谱的蛋白质鉴定和蛋白质表征服务。这些服务包括来自凝胶的蛋白质鉴定，溶液，共免疫沉淀蛋白的鉴定以及蛋白质表征，包括磷酸化位点的映射。最近，根据研究人员的需求，核心基于Amersham的Dige Technique实施了差异蛋白表达分析的新程序。核心由教职员工，核心董事，卡罗尔·帕克（Carol Parker）兼助理主任玛丽亚·沃伦（Maria Warren）博士领导。该核心通过支持21多个赠款提案来为中心增加价值 UNC LCCC成员。核心支持的研究的亮点包括基于核心工作中所做的工作的两份2003年自然出版物：一份自然论文涉及鉴定涉及细胞周期调节的卡林综合体的新成员（对于Y. Xiong博士）；另一个自然出版物涉及鉴定磷酸化位点，这对细胞迁移至关重要，这对转移具有影响（对于K. Jacobson博士）。核心的未来计划包括扩展差异蛋白表达程序，以准确的分子量确定完整蛋白质和持续方法，从而增加了当前程序确定磷酸化和泛素化位点的开发。