Optical monitoring of engineered tissues

工程组织的光学监测

• 批准号: 7559361
• 负责人: IRENE GEORGAKOUDI
• 金额: $ 32.82万
• 依托单位: TUFTS UNIVERSITY MEDFORD
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7559361
• 关键词: Adipose tissue; Animals; Biochemical; Biochemistry; Biocompatible Materials; Biological Markers; Bone Regeneration; Bone Tissue; Cell Differentiation process; Cell Survival; Cell physiology; Cells; Clinical; Collaborations; Collagen; Collection; Computer software; Data; Defect; Deposition; Detection; Development; Engineering; Equilibrium; Extracellular Matrix; Fatty acid glycerol esters; Fluorescence; Funding; Goals; Growth; Histocompatibility Testing; Human; Image; Implant; In Vitro; Knowledge; Light; Mammary gland; Mesenchymal Stem Cells; Metabolic; Methods; Monitor; Morphology; Natural regeneration; Operative Surgical Procedures; Optical Methods; Optics; Osteoporosis; Outcome; Pathway interactions; Patients; Play; Process; Property; Proteins; Public Health; Reconstructive Surgical Procedures; Reporting; Research; Research Personnel; Resolution; Resources; Role; Sampling; Signal Transduction; Silk; Source; Staining method; Stains; Stem cells; Structure; System; Technology; Testing; Time; Tissue Engineering; Tissues; United States National Institutes of Health; Validation; Work; base; bone; bone engineering; cell growth; experience; functional status; imaging modality; implantation; in vivo; innovation; instrumentation; light scattering; mouse model; non-invasive monitor; novel; osteogenic; public health relevance; reconstruction; repaired; scaffold; soft tissue; spectroscopic imaging; stem cell biology; tissue regeneration; tool

DESCRIPTION (provided by applicant): Our long-term goal is to develop non-invasive, optical technologies to monitor the functional development of engineered tissues in vitro and in vivo. The objective of this application is to develop optical biomarkers based on endogenous sources of optical contrast that obviate the use of exogenous stains and can be used to report quantitatively on the biochemical and structural composition of engineered tissues. The proposed studies focus on the characterization of adipose and bone engineered tissues developed from silk scaffolds seeded with human mesenchymal stem cells. The central hypothesis of the application is that linear and non-linear depthresolved imaging methods based on the natural light scattering and fluorescence signatures of cell and matrix components of engineered tissues can be developed to report on the dynamic changes that occur prior to and following implantation of engineered tissues. Our hypothesis is based on preliminary evidence acquired from in vitro samples, which indicate that endogenous optical signals can be used to monitor changes in the biochemistry and morphology of differentiating stem cells, silk scaffolds and deposited collagen. The rationale for the proposed research is that the establishment of non-invasive methods that allow monitoring of the dynamic changes that occur within engineered tissues will play an essential role in the development and optimization of innovative, functional engineered tissue constructs. To achieve our goal we will characterize the endogenous fluorescence and light scattering signals from different cell and matrix components of engineered tissues developed in vitro (Aim 1). We will develop a system that will optimize acquisition of these optical signals from animals (Aim 2) and we will use these biomarkers to characterize non-invasively the integration of these engineered tissues in vivo following implantation either within a mammary fat pad or a cavarial bone defect mouse model (Aim 3). This will be the first time that dynamic monitoring of the biochemical and structural function of implanted engineered tissues is achieved in vivo using non-invasive means based on endogenous optical signals. This proposal is highly relevant to the improvement of public health as it will enable the efficient development of functional bone and adipose engineered tissues. Thus, millions of patients that undergo surgical procedures for the repair or reconstruction of such tissues will ultimately benefit from this work. Public Health Relevance Statement (provided by applicant): This proposal is highly relevant to the improvement of public health as it will enable the efficient development of functional bone and adipose engineered tissues. Thus, millions of patients that undergo surgical procedures for the repair or reconstruction of such tissues will ultimately benefit from this work.

描述（由申请人提供）： 我们的长期目标是开发非侵入性的光学技术，以监测体外和体内工程组织的功能开发。该应用的目的是基于内源性对比来源开发光学生物标志物，以消除外源性污渍的使用，并可用于定量报告工程组织的生化和结构组成。拟议的研究集中于脂肪和骨工程组织的表征，该组织是由用人间质干细胞接种的丝绸支架发展的。该应用的中心假设是，可以开发基于自然光散射和工程组织的细胞和基质组件的线性和非线性深度差异成像方法，以报告在植入工程组织之前和之后发生的动态变化。我们的假设是基于从IN中获得的初步证据 体外样品，表明内源性光学信号可用于监视 分化干细胞，丝绸支架和沉积胶原蛋白的生物化学和形态。拟议研究的基本原理是，建立允许监测工程组织中发生动态变化的非侵入性方法将在创新，功能性工程组织结构的开发和优化中起着至关重要的作用。为了实现我们的目标，我们将表征来自不同细胞和基质组件的内源性荧光和光散射信号在体外开发的工程组织（AIM 1）。我们将开发一个系统，该系统将优化从动物中获取这些光学信号（AIM 2），我们将使用这些生物标志物在植入乳腺脂肪垫或量化的骨缺损小鼠模型中植入后体内这些工程组织在体内的非侵入性表征（AIM 3）。这将是首次使用基于内源性光学信号的非侵入性手段在体内实现植入工程组织的生化和结构功能的动态监测。该建议与公共卫生的改善高度相关，因为它将有效地发展功能性骨和脂肪工程组织。因此，成千上万的患者接受手术治疗以修复或重建此类组织，最终将受益于这项工作。 公共卫生相关性声明（由申请人提供）：该提案与公共卫生的改善高度相关，因为它将有效地发展功能性骨和脂肪工程组织。因此，成千上万的患者接受手术治疗以修复或重建此类组织，最终将受益于这项工作。