THE RESPONSE REGULATOR-LIKE DOMAIN OF RSSB

RSSB 的类似响应调节器的域

• 批准号: 7721245
• 负责人: TANIA A BAKER
• 金额: $ 0.35万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721245
• 关键词: Binding; Computer Retrieval of Information on Scientific Projects Database; DNA-Directed RNA Polymerase; Escherichia coli; Funding; Genes; Grant; Institution; N-terminal; Phase; Phosphotransferases; Research; Research Personnel; Resources; Source; United States National Institutes of Health; base; biological adaptation to stress; genetic regulatory protein; interest; protein-histidine kinase; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RssB is a regulatory protein involved in the stress response in E. coli. It is phosphorylated at Asp57 of its N-terminal domain by the ArcB histidine kinase. In its phosphorylated from RssB binds to sigma-S regulatory subunit of RNA polymerase and targets sigma-S for degradation by ClpXP. In its non- phosphorylated form RssB does not bind sigma-S, allowing sigma-S to accumulate and exert its effect by turning on stationary phase and stress response genes. We are interested in understanding the structural basis for the interaction of RssB with sigma-S, the kinase, and ClpX.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 RssB 是一种参与大肠杆菌应激反应的调节蛋白。它的 N 末端结构域 Asp57 被 ArcB 组氨酸激酶磷酸化。 RssB 磷酸化后与 RNA 聚合酶的 sigma-S 调节亚基结合，并以 sigma-S 为目标，被 ClpXP 降解。在其非磷酸化形式中，RssB 不结合 sigma-S，从而允许 sigma-S 积累并通过打开稳定期和应激反应基因发挥其作用。 我们有兴趣了解 RssB 与 sigma-S、激酶和 ClpX 相互作用的结构基础。