X-RAY CRYSTALLOGRAPHIC STUDIES OF DEOXYCYTIDINE KINASE

脱氧胞苷激酶的 X 射线晶体学研究

• 批准号: 7721218
• 负责人: STEVEN E EALICK
• 金额: $ 0.56万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721218
• 关键词: Computer Retrieval of Information on Scientific Projects Database; DNA; Deoxyadenosines; Deoxycytidine; Deoxycytidine Kinase; Deoxyguanosine; Enzymes; Funding; Grant; Institution; Pathway interactions; Pharmaceutical Preparations; Process; Prodrugs; Rate; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; Uridine Triphosphate; deoxyadenosine; inorganic phosphate; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Deoxycytidine kinase (dCK) is an enzyme involved in DNA salvage pathway. It phosporylates deoxycytidine, deoxyguanosine, and deoxyadenosine using uridine triphosphate as the preferred phosphate donor. dCK has also been shown to activate several antitumor compounds. This activation process is the rate limiting step for several prodrugs. We are determining the crystal structure of dCK with several compounds to increase our understanding of the enzyme and to aid in the discovery of novel drugs.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 脱氧胞苷激酶 (dCK) 是一种参与 DNA 挽救途径的酶。 它使用三磷酸尿苷作为优选的磷酸盐供体来磷酸化脱氧胞苷、脱氧鸟苷和脱氧腺苷。 dCK 还被证明可以激活多种抗肿瘤化合物。 该激活过程是几种前药的限速步骤。 我们正在用几种化合物确定 dCK 的晶体结构，以增加我们对酶的了解并帮助发现新药物。