NE-CAT: A Resource for Advanced Macromolecular Crystallography

NE-CAT：高级高分子晶体学资源

基本信息

批准号：
9904756
负责人：
STEVEN E EALICK
金额：
$ 284.05万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-04-01 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9904756
关键词：
Advisory Committees Aging Area Automatic Data Processing Biological Biological Process Biomedical Research Communities Computer software Crystallography Data Analyses Data Collection Development Drug Design Drug Targeting Education and Outreach Educational workshop Ensure Funding Goals Grouping Hand Healthcare Individual Infrastructure Institution Laboratories Logistics Mainstreaming Maintenance Manuscripts Modification Optics Paper Persons Pharmacologic Substance Photons Productivity Professional Organizations Publications Publishing Recruitment Activity Research Research Personnel Research Project Grants Resources Robotics Roentgen Rays Running Sampling Schedule Site Source Structure Synchrotrons Techniques Technology Time Training Training Activity Training Programs United States National Institutes of Health Universities Work beamline biological systems cohesion computerized data processing data structure design detector experience improved macromolecule meetings member operation outreach outreach program programs repaired structured data three dimensional structure web based interface web site

项目摘要

The main goal of the NE-CAT Resource is to provide user access to state-of-the-art synchrotron beamlines for macromolecular crystallography. NE-CAT began planning and design for two beamlines in 2001. Beamline 24- ID-C became available to the Advanced Photon Source users in 2006 and beamline 24-ID-E became available in 2007. During the ensuing years, beamline improvements have resulted in ultrastable, small X-ray beams ideally suited for challenging problems in macromolecular crystallography. Each beamline is equipped with a high capacity sample automounter and a microdiffractometer. Beamline 24-ID-C features a PILATUS 6MF pixel array detector, and by early 2017 an EIGER 16M pixel array detector will be available on beamline ID-24- E. NE-CAT provides automated software for data collection and analysis (RAPD) and a Web-based interface for remote data collection. We will continue to provide routine maintenance to ensure efficient and reliable data collection, and we propose an upgrade program to maintain state-of-the-art capabilities over the five years covered by this application. NE-CAT has an experienced and dedicated scientific staff who enable access to the beamlines. The staff is available or on call 24/7 to assist users with data collection and processing needs. This highly regarded staff is a major strength of NE-CAT. NE-CAT has an outstanding record of reliability with technical problems rarely interfering with user access. NE-CAT is directed by Dr. Steven Ealick, who has more that 25 years of experience overseeing the operation of synchrotron facilities for macromolecular crystallography. The technical group, led by Deputy Director Dr. Malcolm Capel, and the operations group, led by Associate Director Dr. Kanagalaghatta Rajashankar work closely together to ensure that the needs of the user community are met. Assistant Director Dr. Frank Murphy is responsible for development and maintenance of RAPD. Together, these four individuals make up the NE-CAT management team. NE-CAT beam time is in high demand, and each run all available beamtime is allocated to users. NE-CAT typically hosts more than 1500 users per year from about 160 unique user groups, either on site or remotely. NE-CAT users published about 150 papers during the last year, and they are projected to publish around 170 in 2016. NE-CAT's record of productivity places us among the top of worldwide facilities for macromolecular crystallography. NE-CAT operates an extensive user training program adaptable to first time users, advanced users with technically challenging problems, and everyone in between. A Web site is available to provide all information needed by new or current users. NE-CAT participates in many training and outreach activities including organization of an annual workshop and participation in professional society meetings. The staff works closely with user groups and are often invited to coauthor manuscripts resulting from user assistance.

NE-CAT资源的主要目标是为用户访问对最先进的同步器束线的访问大分子晶体学。 NE-CAT在2001年开始为两个光束线计划和设计。光线线24-- ID-C于2006年可用于高级光子源用户，并且Beamine 24-ID-E可用在2007年。在随后的几年中，光束线的改进导致了超高的小X射线梁理想的适合大分子晶体学中的具有挑战性的问题。每个光束线配备了高容量样品自动遇到和微帧速率计。梁线24-ID-C具有Pilatus 6MF 像素阵列检测器，到2017年初，Eiger 16m像素阵列检测器将在Beamine ID-24-- E. NE-CAT提供用于数据收集和分析（RAPD）和基于Web的界面的自动化软件用于远程数据收集。我们将继续提供常规维护以确保高效可靠的数据收集，我们提出了一个升级计划，以维持五年来最先进的功能被此应用程序涵盖。 NE-CAT has an experienced and dedicated scientific staff who enable access to 梁线。工作人员可用或通话24/7，以帮助用户满足数据收集和处理需求。这位备受推崇的员工是NE-CAT的主要优势。 NE-CAT拥有可靠性的出色记录技术问题很少干扰用户访问。 Ne-cat由史蒂文·埃里克（Steven Ealick）博士执导，他有更多那25年的经验，负责大分子的同步器设施的运行晶体学。技术小组由副主任马尔科姆·卡普尔（Malcolm Capel）和运营小组领导副主任Kanagalaghatta Rajashankar博士紧密合作，以确保需要满足用户社区。助理主任弗兰克·墨菲（Frank Murphy）博士负责开发和维护 Rapd。这四个人在一起组成了NE-CAT管理团队。 Ne-cat梁时间在需求很高，每个运行所有可用的beamtime都分配给用户。 NE-CAT通常托管超过每年1500个用户来自现场或远程的大约160个独特的用户组。 NE-CAT用户发布了去年大约有150篇论文，预计在2016年发表左右。生产力使我们跻身于大分子晶体学的全球设施中。 ne-cat 操作一项广泛的用户培训计划，适用于首次用户，具有技术上的高级用户具有挑战性的问题，以及介于两者之间的每个人。可以使用网站提供所有需要的信息新的或当前的用户。 NE-CAT参加了许多培训和外展活动，包括年度研讨会和参加专业社会会议。员工与用户组紧密合作并经常被邀请到由用户帮助而产生的合着者手稿。