NE-CAT: A Resource for Advanced Macromolecular Crystallography

NE-CAT：高级高分子晶体学资源

基本信息

批准号：
10379339
负责人：
STEVEN E EALICK
金额：
$ 277.31万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-04-01 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10379339
关键词：
Advisory Committees Aging Area Automatic Data Processing Biological Biological Process Biomedical Research Communities Computer software Crystallography Data Analyses Data Collection Development Drug Design Drug Targeting Education and Outreach Educational workshop Ensure Funding Goals Grouping Hand Healthcare Individual Infrastructure Institution Laboratories Logistics Mainstreaming Maintenance Manuscripts Modification Optics Paper Persons Pharmacologic Substance Photons Productivity Professional Organizations Publications Publishing Recruitment Activity Research Research Personnel Research Project Grants Resources Robotics Roentgen Rays Running Sampling Schedule Site Source Structure Synchrotrons Techniques Technology Time Training Training Activity Training Programs United States National Institutes of Health Universities Work beamline biological systems cohesion computerized data processing data structure design detector experience improved macromolecule meetings member operation outreach outreach program programs repaired three dimensional structure web based interface web site

项目摘要

The main goal of the NE-CAT Resource is to provide user access to state-of-the-art synchrotron beamlines for macromolecular crystallography. NE-CAT began planning and design for two beamlines in 2001. Beamline 24- ID-C became available to the Advanced Photon Source users in 2006 and beamline 24-ID-E became available in 2007. During the ensuing years, beamline improvements have resulted in ultrastable, small X-ray beams ideally suited for challenging problems in macromolecular crystallography. Each beamline is equipped with a high capacity sample automounter and a microdiffractometer. Beamline 24-ID-C features a PILATUS 6MF pixel array detector, and by early 2017 an EIGER 16M pixel array detector will be available on beamline ID-24- E. NE-CAT provides automated software for data collection and analysis (RAPD) and a Web-based interface for remote data collection. We will continue to provide routine maintenance to ensure efficient and reliable data collection, and we propose an upgrade program to maintain state-of-the-art capabilities over the five years covered by this application. NE-CAT has an experienced and dedicated scientific staff who enable access to the beamlines. The staff is available or on call 24/7 to assist users with data collection and processing needs. This highly regarded staff is a major strength of NE-CAT. NE-CAT has an outstanding record of reliability with technical problems rarely interfering with user access. NE-CAT is directed by Dr. Steven Ealick, who has more that 25 years of experience overseeing the operation of synchrotron facilities for macromolecular crystallography. The technical group, led by Deputy Director Dr. Malcolm Capel, and the operations group, led by Associate Director Dr. Kanagalaghatta Rajashankar work closely together to ensure that the needs of the user community are met. Assistant Director Dr. Frank Murphy is responsible for development and maintenance of RAPD. Together, these four individuals make up the NE-CAT management team. NE-CAT beam time is in high demand, and each run all available beamtime is allocated to users. NE-CAT typically hosts more than 1500 users per year from about 160 unique user groups, either on site or remotely. NE-CAT users published about 150 papers during the last year, and they are projected to publish around 170 in 2016. NE-CAT's record of productivity places us among the top of worldwide facilities for macromolecular crystallography. NE-CAT operates an extensive user training program adaptable to first time users, advanced users with technically challenging problems, and everyone in between. A Web site is available to provide all information needed by new or current users. NE-CAT participates in many training and outreach activities including organization of an annual workshop and participation in professional society meetings. The staff works closely with user groups and are often invited to coauthor manuscripts resulting from user assistance.

NE-CAT 资源的主要目标是为用户提供最先进的同步加速器光束线大分子晶体学。 NE-CAT 于 2001 年开始规划和设计两条光束线。Beamline 24- ID-C 于 2006 年可供高级光子源用户使用，光束线 24-ID-E 也可供使用 2007 年。在接下来的几年里，光束线的改进带来了超稳定的小型 X 射线束非常适合解决大分子晶体学中具有挑战性的问题。每条光束线都配备有高容量样品自动安装机和微衍射仪。 Beamline 24-ID-C 采用 PILATUS 6MF 像素阵列探测器，到 2017 年初，光束线 ID-24 上将提供 EIGER 16M 像素阵列探测器 E. NE-CAT 提供用于数据收集和分析 (RAPD) 的自动化软件以及基于 Web 的界面用于远程数据收集。我们将持续提供日常维护，确保数据高效可靠集合，我们提出了一个升级计划，以在五年内保持最先进的能力本申请涵盖。 NE-CAT 拥有经验丰富、敬业的科研人员，他们能够光束线。工作人员 24/7 随时待命，协助用户满足数据收集和处理需求。这些备受尊敬的员工是 NE-CAT 的主要优势。 NE-CAT 拥有出色的可靠性记录技术问题很少干扰用户访问。 NE-CAT 由 Steven Ealick 博士指导，他拥有更多拥有 25 年监督高分子同步加速器设施运行的经验晶体学。技术组由副主任 Malcolm Capel 博士领导，运营组由副主任 Malcolm Capel 博士领导。副主任 Kanagalaghatta Rajashankar 博士密切合作，确保满足用户社区得到满足。助理主任 Frank Murphy 博士负责开发和维护 RAPD 的。这四人共同组成了 NE-CAT 管理团队。 NE-CAT 波束时间为高需求，并且每次运行所有可用的波束时间都分配给用户。 NE-CAT 通常托管超过每年来自约 160 个独特用户组的 1500 名用户，无论是现场还是远程。 NE-CAT用户发布去年他们发表了约 150 篇论文，预计 2016 年将发表约 170 篇论文。 NE-CAT 的记录生产力的提高使我们跻身于全球大分子晶体学设施的顶尖之列。 NE-CAT 运营广泛的用户培训计划，适合初次用户、技术熟练的高级用户具有挑战性的问题，以及介于两者之间的每个人。一个网站可以提供所需的所有信息新用户或现有用户。 NE-CAT 参与许多培训和外展活动，包括组织年度研讨会和参加专业协会会议。工作人员与用户群体密切合作并经常被邀请共同撰写由用户协助产生的手稿。