SINGLE MOLECULE ELECTRICAL RECORDING WITH NANOPORE DEVICE

使用纳米孔器件进行单分子电记录

• 批准号: 7723596
• 负责人: Aleksei Aksimentiev
• 金额: $ 4.74万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723596
• 关键词: Characteristics; Collaborations; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Sequence; Devices; Electrical Engineering; Funding; Genotype; Grant; Institution; Length; Membrane; Modeling; Physics; Research; Research Personnel; Resolution; Resources; Signal Transduction; Silicon Dioxide; Source; Stretching; Technology; United States National Institutes of Health; Work; base; electric field; improved; nanopore; nanoscale; single molecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A nano-meter size pore, a so-called nanopore, can be manufactured in thin inorganic membranes. The most important application for nanopores is DNA sequencing: under the influence of an electric field, DNA translocates through the nanopore, producing electrical signals characteristic of the sequence and length of the DNA strand. Current synthetic nanopores can not reach single-base resolution yet; however, they are among the most promising technologies for cheap DNA sequencing. The Resource has been working in close collaboration with electrical engineers (Gregory Timp and Jean-Pierre Leburton) to understand the physics of synthetic nanopores and improve their resolution. Atomic-scale modeling was carried out in three directions: (i) genotyping with synthetic nanopore; (ii) stretching/ unzipping DNA hairpins with a synthetic nanopore; (iii) sensing DNA sequence with a nanopore capacitor; (iv) modeling of ionic current through silica nanopores (http://www.ks.uiuc.edu/Research/nanopore/).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 纳米米大小的孔，一种所谓的纳米孔，可以用薄的无机膜制造。纳米孔的最重要应用是DNA测序：在电场的影响下，DNA通过纳米孔易位，产生了DNA链序列和长度的电信号的特征。 当前的合成纳米孔无法达到单碱基分辨率。但是，它们是廉价DNA测序最有前途的技术之一。 该资源一直与电气工程师（Gregory Timp和Jean-Pierre Leburton）密切合作，以了解合成纳米孔的物理学并改善其分辨率。 原子尺度建模是在三个方向上进行的：（i）与合成纳米孔进行基因分型； （ii）用合成纳米孔拉伸/解压缩DNA发夹； （iii）用纳米电容器传感DNA序列； （iv）通过二氧化硅纳米孔（http://www.ks.uiuc.edu/research/nanopore/）对离子电流进行建模。