MONITORING OF MRFP-CAMKIIA-GFP ACTIVITY USING FLIM SYSTEM

使用 FLIM 系统监测 MRFP-CAMKIIA-GFP 活动

• 批准号: 7724060
• 负责人: YASUNORI HAYASHI
• 金额: $ 0.59万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724060
• 关键词: Calcium; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Resonance Energy Transfer; Funding; Grant; Institution; Ionophores; Learning; Monitor; Proteins; Research; Research Personnel; Resources; Source; System; United States National Institutes of Health; fluorophore; memory process; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The FRET probe, mRFP-CamKIIa-GFP construct is made to study the activity of CamKIIa, a critical protein in the process of memory formation and learning. By adding calcium ionophore, we can activate this protein. Upon activation, CamKIIa will have a conformational change which decreases FRET between the two fluorophores. Using the FLIM system in Dr. Gratton's lab, we can monitor the change in FRET. eGFP and mRFP-CamKIIa-GFP-DD mutant (always FRET) are used to serve as negative and positive controls.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 FRET探测器MRFP-CAMKIIA-GFP构建体是为了研究CAMKIIA的活性，CamkiiA是一种关键的记忆形成和学习过程中的关键蛋白质。通过添加钙离子载体，我们可以激活该蛋白质。激活后，CaMKIIA将具有构象变化，可减少两个荧光团之间的fret。使用Gratton博士实验室中的FLIM系统，我们可以监视FRET的变化。 EGFP和MRFP-CAMKIIA-GFP-DD突变体（始终fret）用于负面和阳性对照。