CONFOCAL MICROFLUOROMETRY & MICROSCOPY CORE

共焦显微荧光测定

• 批准号: 7720921
• 负责人: A. Philine Wangemann
• 金额: $ 15.9万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720921
• 关键词: 3-Dimensional; Calcium Signaling; Centers of Research Excellence; Color; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Cytophotometry; Epithelial; Equipment; Fingerprint; Fluorescence Resonance Energy Transfer; Funding; Grant; Imagery; Institution; Label; Mentors; Microscopy; Research; Research Personnel; Resolution; Resources; Scanning; Source; Techniques; Tissues; Training; United States National Institutes of Health; instrumentation; reconstruction; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The COBRE Confocal Microfluorometry and Microscopy Core facility provides the instrumentation, expertise, training and mentoring in confocal microfluorometry and microscopy necessary for the advancement of all COBRE projects. The projects are critically dependent on: microfluorometry of fast calcium signals in cultured and native epithelial tissues; subcellular visualization of molecules, including the 3-dimensional reconstruction of labeling; colocalization experiments where maximal resolution and color separation through emission fingerprinting are necessary; and advanced colocalization techniques, such as FRET, for which the emission-scanning capabilities of our equipment is critical.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 COBRE 共焦显微荧光测定和显微镜核心设施提供共焦显微荧光测定和显微镜方面的仪器、专业知识、培训和指导，以推进所有 COBRE 项目。这些项目主要依赖于：培养和天然上皮组织中快速钙信号的微荧光测定；分子的亚细胞可视化，包括标记的 3 维重建；共定位实验，其中需要通过发射指纹进行最大分辨率和颜色分离；以及先进的共定位技术，例如 FRET，我们设备的发射扫描能力对此至关重要。