LIPOSOME-ENCAPSULATED NANOSHELLS

脂质体封装的纳米壳

• 批准号: 7721143
• 负责人: NAOMI HALAS
• 金额: $ 1.62万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721143
• 关键词: Anti-Inflammatory Agents; Anti-inflammatory; Arthritis; Aspirin; Binding; Biological Process; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; Drug Delivery Systems; Drug usage; Electrostatics; Encapsulated; Fever; Funding; Gastric mucosa; Gold; Grant; Image; Institution; Lipids; Liposomes; Measurement; Mechanics; Membrane; Membrane Lipids; Microscopic; Minor; Monitor; Optics; Pain; Phospholipids; Property; Protein Chemistry; Research; Research Personnel; Resources; Salicylic Acid; Salicylic Acids; Sampling; Signal Transduction; Silicon Dioxide; Source; Surface; System; Thick; Ulcer; United States National Institutes of Health; insight; member; nanoparticle; nanoscale; nanoshell; response; salicylate; tool; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Salicylate is the active metabolite of aspirin and a member of the popular non-steroidal anti-inflammatory drugs (NSAIDs) used to treat fever, pain and arthritis. However, it is known to cause ulcers, which could be explained by the ability of the molecule to disrupt the phospholipid layer covering the gastric mucosa. Because mechanical properties of a lipid membrane can be altered by minor changes in the chemistry of the proteins or lipids that compose the membrane, and because electrostatics is the most dominant force at the nanoscale, it is believed that more detailed measurements of salicylate-lipid interactions will provide insights into these fundamental biological processes. Surface-Enhanced Raman Scattering (SERS) response of gold nanoshells, which are tunable optical nanoparticles consisting of a dielectric (silica) core and a thin metallic (gold) shell, can be a very useful tool to probe salicylate membrane interactions. The optical resonance of nanoshells gives rise to an intense optical field at the surface of the nanoparticle. The high optical intensities at the nanoshell surface can be used to enhance the chemical spectroscopic signal of salicylate, a Raman-active molecule, which when embedded in a lipid membrane on the surface of the nanoshell should yield a strong SERS signal. Thus, SERS will be useful to probe lipid membrane properties and monitor the insertion of salicylic acid molecules into the lipid membrane. SERS measurements of inserted salicylate hinges on successful liposome- encapsulation of nanoshells. This requires extensive microscopic characterization of the lipid-nanoshell interacting system. CryoEM measurements can provide evidence of liposome-encapsulation of nanoshells and can help establish the thickness of the lipid membrane formed around the nanoshells. Therefore, cryoEM imaging is essential to the progress of this project. Imaging of the prepared liposome-nanoshell samples will be conducted at the cryoEM facility at NCMI. Results from our studies will relate our continuum micromechanical measurements to molecular interactions. In addition to studying the effect salicylate has on membrane dynamics, nanoshell-encapsulated liposomes may provide a vehicle for intracellular uptake of nanoshells which can be useful for drug delivery.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 水杨酸酯是阿司匹林的活性代谢产物，也是用于治疗发烧，疼痛和关节炎的流行非甾体类抗炎药（NSAIDS）的成员。 但是，已知会引起溃疡，这可以通过分子破坏覆盖胃粘膜的磷脂层的能力来解释。由于脂质膜的机械性能可以通过构成膜的蛋白质或脂质的化学变化来改变，并且由于静电是纳米级最主要的力量，因此人们认为，更详细的水杨酸脂质相互作用的测量结果将为这些碱性生物过程提供洞察力。 金纳米壳的表面增强拉曼散射（SERS）响应是由介电（硅）芯和薄金属（金）壳组成的可调光学纳米颗粒，可以是探测水杨酸酯膜相互作用的非常有用的工具。 纳米壳的光学共振产生了纳米颗粒表面的强烈光场。 纳米壳表面的高光学强度可用于增强水杨酸水杨酸酯的化学光谱信号，水杨酸酯是一种拉曼活性分子，当将其嵌入纳米壳表面的脂质膜中时，该信号应产生强烈的SERS信号。 因此，SER对于探测脂质膜特性和监测水杨酸分子插入脂质膜将是有用的。 插入水杨酸酯取决于纳米壳的成功脂质体封装的SERS测量。 这需要脂质 - 纳米什尔相互作用系统的广泛微观表征。冷冻测量值可以提供纳米丝脂质体囊化的证据，并有助于确定纳米壳周围形成的脂质膜的厚度。因此，冷冻成像对于该项目的进展至关重要。 准备好的脂质体纳米壳样品的成像将在NCMI的冷冻设施进行。我们的研究结果将使我们的连续微力测量与分子相互作用有关。 除了研究水杨酸盐对膜动力学的影响外，纳米壳封闭的脂质体还可以为细胞内摄取纳米壳提供载体，这对于药物递送很有用。