Translation Grant for The HIPER project - Bringing the NMR Paradigm to ESR

HIPER 项目翻译资助 - 将 NMR 范式引入 ESR

• 批准号: EP/F039034/1
• 负责人: Graham Smith
• 金额: $ 126.71万
• 依托单位: University of St Andrews
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FF039034%2F1
• 关键词: Translation; Grant; HIPER; project; Bringing

In the original Basic Technology program Bringing the NMR Paradigm to ESR technology was developed to deliver complex sequences of very high power mm-wave pulses with sub-nanosecond resolution to a small sample in a strong magnetic field and measure a response from the sample 13 orders of magnitude lower in power, within 1 nanosecond of the final pulse. This was widely regarded as impossible by many leading members of the ESR instrumentation community three years ago. However, after two years developing the necessary mm-wave technologies and one year integrating them into a fully working system, the instrument is now demonstrating these very high levels of performance.The success of that program now opens up major new opportunities in the understanding of fast chemical and biochemical processes and in dramatically improving the sensitivity and resolution of techniques to understand local structure around paramagnetic centres, which often play a central role in determining the catalytic, enzymatic, electronic and optical properties of important chemical, biological and technological systems. It also promises to significantly improve the sensitivity of Site-Directed Spin Labelling techniques that promise to become a generic tool in the understanding of the structure, dynamics, conformational changes, interactions and function of important biomolecules in complex biomolecular processes. The aim of this Translation Grant is to provide underpinning but flexible support that will allow us to fully exploit this technological breakthrough via a series of pilot projects and collaborations.

在最初的基础技术计划中，开发了将 NMR 范式引入 ESR 技术，以将具有亚纳秒分辨率的极高功率毫米波脉冲的复杂序列传送到强磁场中的小样品，并测量样品 13 阶的响应功率幅度较低，在最终脉冲的 1 纳秒内。三年前，ESR 仪器界的许多领先成员普遍认为这是不可能的。然而，经过两年开发必要的毫米波技术并将其集成到一个完全工作的系统中一年后，该仪器现在展示了这些非常高水平的性能。该计划的成功现在为理解毫米波技术开辟了重大的新机遇。快速化学和生化过程，并显着提高技术的灵敏度和分辨率，以了解顺磁中心周围的局部结构，这通常在确定重要化学、生物和技术系统的催化、酶促、电子和光学特性方面发挥核心作用。它还有望显着提高定点自旋标记技术的灵敏度，该技术有望成为理解复杂生物分子过程中重要生物分子的结构、动力学、构象变化、相互作用和功能的通用工具。该翻译补助金的目的是提供基础但灵活的支持，使我们能够通过一系列试点项目和合作充分利用这一技术突破。

项目成果

Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba.

二聚体-二聚体堆积相互作用对于古细菌染色质蛋白 Alba 的核酸结合非常重要。

• DOI: http://dx.10.1042/bj20091841
• 发表时间: 2010
• 期刊: The Biochemical journal
• 作者: Jelinska C

Compact Corrugated Feedhorns With High Gaussian Coupling Efficiency and $-60\;\text{dB}$ Sidelobes

具有高斯耦合效率和 $-60; ext{dB}$ 旁瓣的紧凑型波纹馈源喇叭

• DOI: 10.1109/tap.2016.2543799
• 发表时间: 2016-03-18
• 期刊: IEEE Transactions on Antennas and Propagation
• 影响因子: 5.7
• 作者: J. McKay;D. Robertson;Peter J. Speirs;R. Hunter;R. Wylde;Graham M. Smith
• 通讯作者: Graham M. Smith

Analysis of the Intrinsically Disordered N-Terminus of the DNA Junction-Resolving Enzyme T7 Endonuclease I: Identification of Structure Formed upon DNA Binding.

DNA 连接解析酶 T7 核酸内切酶 I 本质上无序的 N 末端分析：鉴定 DNA 结合时形成的结构。

• DOI: http://dx.10.1021/acs.biochem.6b00242
• 发表时间: 2016
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Freeman AD

The histone chaperones Nap1 and Vps75 bind histones H3 and H4 in a tetrameric conformation.

组蛋白伴侣 Nap1 和 Vps75 以四聚体构象结合组蛋白 H3 和 H4。

• DOI: http://dx.10.1016/j.molcel.2011.01.025
• 发表时间: 2011
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Bowman A

Orientation selection in high-field RIDME and PELDOR experiments involving low-spin CoII ions.

涉及低自旋 CoII 离子的高场 RIDME 和 PELDOR 实验中的方向选择。

• DOI: http://dx.10.1039/c7cp07248a
• 发表时间: 2018
• 期刊: PCCP
• 作者: Giannoulis A