INTERACTIONS BETWEEN PROTEINS OF THE MEIOTIC SPINDLE POLE BODY

减数分裂纺锤体蛋白质之间的相互作用

• 批准号: 7723749
• 负责人: Aaron M Neiman
• 金额: $ 1.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723749
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Electron Microscopy; Funding; Grant; Growth; Institution; Meiosis; Membrane; Methodology; Microtubules; Proteins; Reproduction spores; Research; Research Personnel; Resources; Site; Source; Structure; United States National Institutes of Health; base; spindle pole body; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During meiosis, the outer plaque of the spindle pole body (SPB) is reorganized.The structure is converted from a site of microtubule growth to one of membrane formation and anchorage. These membranes, termed prospore membranes, are essential for the formation of spores. Electron microscopy analysis reveals a very highly organized structure for the meiotic outer plaque, and two-hybrid analyses have identified many interactions between these outer plaque proteins. However, precisely how these proteins are organized with respect to each other within the outer plaque is not known. The arrangement of these proteins within the outer plaque will be explored by FRET-based methodology.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在减数分裂过程中，重组纺锤体体（SPB）的外斑块。结构从微管生长的位点转化为膜形成和锚固之一。这些膜，称为牙膜膜，对于孢子的形成至关重要。电子显微镜分析揭示了减数分裂外斑块的组织非常高度组织的结构，并且两个杂交分析已经确定了这些外斑块蛋白之间的许多相互作用。然而，尚不清楚这些蛋白质在外斑块中相对于彼此之间的组织方式尚不清楚。这些蛋白质在外部斑块中的排列将通过基于FRET的方法探索。