MRI OF INSECT FLIGHT MUSCLE IN A BEETLE

甲虫昆虫飞行肌的 MRI

• 批准号: 8171630
• 负责人: MICHAEL REEDY
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171630
• 关键词: Abdomen; Architecture; Arizona; Books; Chest; Computer Retrieval of Information on Scientific Projects Database; Dissection; Eating; Electron Microscopy; Family; Female; Fiber; Flying body movement; Funding; Gases; Grant; Image; In Situ; Insecta; Institution; Larva; Leg; Length; Life; Magnetic Resonance Imaging; Muscle; Nitrogen; Order Coleoptera; Plant Roots; Research; Research Personnel; Resolution; Resources; Source; Thick; United States National Institutes of Health; Water; Width; flyer; hatching; interest; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Body length Body width Body thickness Male 70 mm 20 mm 15 mm Female 70-75 mm 20-22 mm 1) Image 3D internal architecture of flight muscle in thorax (and include abdomen in MRI) of a large beetle known to be a strong flyer (palo verde root borers (Derobrachus geminatus) just collected and fedexed from Tucson, Arizona). The size of the male beetle's body is 70 mm long, 20 mm wide and 15 mm thick. Legs and antennae can be trimmed or removed if not easily tucked in alongside the body of a dead beetle. I want the MRI in order to have an in situ 3D view of the flight muscle undisturbed by dissection. 100 um resolution is ok. 10 um might be interesting if avsilable and 100 um already booked up. Beetles are so heavily sclerotized that gentle flight muscle dissection is very difficult; I have never suceeded with random-catch local NC beetles. Beetles are the last of 4 families of flying insects whose shared type of flight muscle I want to study by electron microscopy and fiber x-ray diffraction. I will asphyxiate a beetle with HCN or nitrogen gas just before starting MRI. Only the larvae eat. The male beetles are not known to eat after hatching and flying about to find and impregnate females. I don't know yet how long they can live on water alone, maybe less than a week.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 体长的身体宽度身体厚度 雄性70毫米20毫米15毫米 女性70-75毫米20-22毫米 1）图像3D胸腔中的飞行肌肉的内部建筑（并包括MRI中的腹部），这是一个大型甲虫，已知是强大的传单（Palo Verde Root Borers（derobrachus geminatus）），刚刚从亚利桑那州图森收集并结束了Fedex）。 雄性甲虫的身体的大小长70毫米，宽20毫米，厚15毫米。 如果不容易将死甲虫的身体塞入腿部，则可以修剪或去除腿和触角。 我想要MRI，以便对不受解剖不受干扰的飞行肌肉进行原位3D视图。 100 UM分辨率还可以。 10嗯，如果有可见的，100 um已经预订，可能会很有趣。甲虫是如此严重的硬化，以至于轻柔的飞行肌肉剖析非常困难。我从来没有使用随机捕获的本地NC甲虫。 甲虫是四个飞行昆虫家族中的最后一个，我想通过电子显微镜和纤维X射线衍射研究，它们的共同类型的飞行肌肉。在开始MRI之前，我将用HCN或氮气窒息甲虫。只有幼虫吃。 雄性甲虫在孵化和飞行以寻找和浸渍雌性之后就吃饭。 我还不知道他们可以独自在水上生活多久，也许不到一个星期。