GD STAINING/MRI FOR FLIGHT MUSCLES ANATOMY IN FORMALIN-FIXED LARGE BEETLE

福尔马林固定大甲虫飞行肌肉解剖的 GD 染色/MRI

• 批准号: 8363195
• 负责人: MICHAEL REEDY
• 金额: $ 0.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363195
• 关键词: Abdominal Cavity; Aldehydes; Anatomy; Anterior; Cephalic; Chest; Complement; Deglutition; Dissection; Female; Fiber; Formalin; Funding; Gastrointestinal tract structure; Grant; Image; In Situ; Magnetic Resonance Imaging; Microscopy; Microtomy; Muscle; Myofibrils; National Center for Research Resources; Oral cavity; Order Coleoptera; Plant Roots; Plastic Embedding; Positioning Attribute; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Ringer' s solution; Source; Staining method; Stains; Structure; Thoracic cavity structure; United States National Institutes of Health; cost; light microscopy; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Gd stain and MRI image of anterior 2/3 of undissected large female beetle (Palo Verde Root Borer (Derobrachus sp) from Tucson, AZ), previously fixed by (and still in) 10% formalin in Ringer solution. This will complement July 2009 MRI study and later dissections of unfixed, unstained male & female beetles of same species. Objectives are twofold: 1) to image position and fiber size & orientation of lomgitudinal and dorso-ventral flight muscles in thorax, and 2) to verify apparent and surprisig absence of a) functional mouth parts, b) swallowing passage (esophgus) and any sign of GI tract in narrow cephalic and spacious thoracic and abdominal cavities while undisturbed by any prior invasive dissection. Want to discuss high-resolution MRI imaging of aldehyde-fixed flight muscle in situ in thorax from a giant waterbug, perhaps with Gd-staining, to complement my year 2000 study of unfixed flight muscle in situ in a whole waterbug. These muscles show long-range crystallinity by x-ray microbeam diffractio, but not by EM thin sectioning; We want to do MRI and then plastic embed and do light microscopy of unperturbed in-situ structure of whole flight muscle to see if the long-range crystalline and the implied absence of all or any twisitng of myofibrils can be carefully preserved and visualized by thin-section EM.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 未分解的大雌性甲虫（来自亚利桑那州图森图森）前2/3的GD染色和MRI图像（Palo Verde root borer（derobrachus sp））以前由Ringer溶液中的（仍在（仍在）10％的福尔马林中固定。这将补充2009年7月的MRI研究，并随后对同一物种的未固定，未染色的雄性和雌性甲虫进行解剖。 目的是双重的：1）图像位置和纤维尺寸和纤维尺寸和方向，胸腔中的lomgitudinal和dorso-dentral飞行肌肉，以及2）验证明显的和惊奇的缺乏a）功能性嘴部零件，b）吞咽通道（食管）在狭窄的头顶和宽敞的胸腔和腹腔中，gi道的迹象，同时不受任何先前的侵入性解剖的干扰。 想讨论从一个巨大的供水物（也许有GD染色）的甲醛固定飞行肌肉原位的高分辨率MRI成像，以补充我2000年对整个水泡中未用的飞行肌肉的研究。 这些肌肉通过X射线微束衍射显示出远距离的结晶度，但不能通过EM薄截面。我们想进行MRI，然后嵌入塑料并进行整个飞行肌肉未扰动的原位结构的光学显微镜，以查看是否可以仔细地保存远距离的晶体和隐含的全部或任何肌原纤维的暗示，并通过薄薄的方式可视化 - 分段EM。