TANDEM QUADRUPOLE MASS SPECTROMETRY OF SULFATIDES

硫化物的串联四极杆质谱法

• 批准号: 7721460
• 负责人: FONG-FU HSU
• 金额: $ 0.89万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721460
• 关键词: Class; Computer Retrieval of Information on Scientific Projects Database; Electrospray Ionization; Fatty Acids; Funding; Grant; Hand; Institution; Ions; Location; Mass Spectrum Analysis; Molecular; Process; Research; Research Personnel; Resources; Scanning; Source; Staging; Structure; Sulfoglycosphingolipids; United States National Institutes of Health; analog; base; hydroxy fatty acid; mass spectrometer; tandem mass spectrometry; unsaturated bonds

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sulfatides yield intense deprotonated molecular species ([M - H]-) in negative-ion mode by electrospray ionization. The product-ion spectra of the [M - H]- ions of sulfatides obtained with a tandem quadrupole and with an ion-trap mass spectrometers contain abundant fragment ions informative for structural determination of the long-chain base and the fatty acid constituent including the location of its unsaturated bond. Identifications of sulfatides in mixtures, or specific sulfatide subclasses that are varied by long-chain base, or differentiation of sulfatide class with a-hydroxy fatty acid substituent from that with non-hydroxy fatty acid group can be facilitated by precursor ion or constant neutral loss scans. While CAD product-ion spectra of the [M - H]- ions are useful for differentiation of isomeric structures, the mechanisms underlying the fragmentation processes for sulfatides under low-energy CAD can be unveiled by multiple-stage ion-trap tandem mass spectrometry of various sulfatides, including their H-D exchanged analogs. The sensitivity of sulfatides observed as the [M + Na]+ ions by ESI in the positive-ion mode, on the other hand, is poor. The product-ion spectra of the [M + Na]+ ions of sulfatides contain very few structurally informative ions and are not useful for structural identification.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 硫化物通过电喷雾电离在负离子模式下产生强烈的去质子化分子物种（[M-H] - ）。用串联四极杆和带有离子陷阱质谱仪获得的[M-H] - 离子的产物离子光谱包含丰富的片段离子，以确定长链碱和脂肪酸组成部分，包括其不饱和键的位置。通过长链碱而变化的混合物中亚硫酸盐或特异性亚硫酸亚类的鉴定，或用A-羟基脂肪酸取代与非羟基脂肪酸基团的分化可以通过前体离子或恒定的中性损失扫描来促进。虽然[M-H]离子的CAD产物离子光谱可用于分化异构体结构，但在低能CAD下的硫化物片段化过程的基础机制可以通过多阶段离子陷阱串联串联量质谱法推测，包括其H-D交换的H-D交换类似物。另一方面，在阳性离子模式下ESI观察到的硫酸硫化物的敏感性很差。硫酸硫化物的[M + Na] +离子的产物离子光谱含有很少的结构信息性离子，并且对结构鉴定没有用。