STRUCTURAL CHARACTERIZATION OF PHOSPHATIDYL-MYO-INOSITOL MANNOSIDES FROM

磷脂酰肌醇甘露糖苷的结构表征

• 批准号: 7953929
• 负责人: FONG-FU HSU
• 金额: $ 1.28万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953929
• 关键词: 1,2-diacylglycerol; Acylation; Calmette-Guerin Bacillus; Complex; Computer Retrieval of Information on Scientific Projects Database; Diglycerides; Fatty Acids; Funding; Glycerol; Glycosides; Grant; Inositol; Institution; Ions; Mannosides; Mass Spectrum Analysis; Methods; Research; Research Personnel; Resources; Source; Staging; Structure; United States National Institutes of Health; Vertebral column; base; biomedical resource

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The multiple-stage ion-trap mass spectrometric approaches towards to the structural characterization of the monoacyl-PIM (triacylated PIM) and the diacyl-PIM (tetracylated PIM), namely, the PIM (diacylated PIM) consisting of one or two additional fatty acid substituents attached to the glycoside, respectively, were described. While the assignment and confirmation of the fatty acid substituents on the glycerol backbone can be easily achieved by the methods described in the previous article, the identity of the glycoside moiety and its acylation state can be determined by the observation of a prominent acylglycoside ion arising from cleavage of the diacylglycerol moiety ([M - H - diacylglycerol]-) in the MS2 spectra of monoacyl-PIM and diacyl-PIM. The distinction of the fatty acid substituents on the 2-O-mannoside (i.e, R3CO2H) from that on the inositol (i.e., R4CO2H) is based on the findings that the MS3 spectrum of [M - H - diacylglycerol]- contains a prominent ion arising from further loss of the fatty acid at the 2-O-mannoside (i.e, the [M - H - diacylglycerol - R3CO2H]- ion); while the ion arising from loss of the fatty acid substituent at the inositol (i.e, the [M - H - diacylglycerol - R4CO2H]- ion) is of low abundance. The fatty acyl moiety on the inositol can also be identified by the product-ion spectrum from MS4 of the [M - H - diacylglycerol R3CO2H]- ion, which gives rise to a prominent ion corresponding to loss of R4CO2H. An [M - H - acylmannose]- ion was also observed in the MS2 spectra and thus, the identity of the fatty acid substituent attached to 2-O-mannoside can be confirmed. The combined information obtained from the multiple-stage product-ion spectra from MS2, MS3, and MS4 permit the assignment of the complex structures of monoacyl-PIMs and diacyl-PIMs in a mixture isolated from M. bovis Bacillus Calmette Guerin.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 多阶段离子陷阱质谱方法，用于单纤维 - PIM（三酰基PIM）和二酰基 - 二酰基 - 二酰二酰二酰二酰基PIM（四酰基PIM）的结构表征，即分别描述了一个附属于glycoside的pim（二酰基PIM）（二酰基化的PIM）。 While the assignment and confirmation of the fatty acid substituents on the glycerol backbone can be easily achieved by the methods described in the previous article, the identity of the glycoside moiety and its acylation state can be determined by the observation of a prominent acylglycoside ion arising from cleavage of the diacylglycerol moiety ([M - H - diacylglycerol]-) in the MS2 spectra Monoacyl-Pim和二酰基PIM。脂肪酸取代于2-O-甘露糖苷（即R3CO2H）与肌醇（即R4CO2H）上的区别是基于发现的结果，即[M-H-H-二酰基甘油]的MS3频谱（MS3频谱）包括一个突出的离子损失。二酰基甘油-R3CO2H] - 离子）;而在肌醇（即[M -H -H-二酰基甘油-R4CO2H] - 离子）上脂肪酸取代基的损失产生的离子是低丰度。肌醇上的脂肪酰基部分也可以通过[M -H -H-二酰基甘油R3CO2H] - 离子的MS4的产物离子光谱鉴定，这导致了与R4CO2H损失相对应的显着离子。在MS2光谱中也观察到了[M-H-H-酰基甘露糖] - 离子，因此，可以确认附着在2-O-甘露糖苷上的脂肪酸取代基的身份。从MS2，MS3和MS4从多阶段的产品-ION光谱中获得的组合信息允许在从牛乳杆菌Calsette Guerin分离的混合物中分配Monoacyl-Pims和Diacyl-PIM的复杂结构。