CHAR OF CARDIOLIPIN BY TQ & MSQ ION-TRAP MASS SPEC WITH ELECTROSPRAY IONIZ

TQ 的心磷脂字符

• 批准号: 7721453
• 负责人: FONG-FU HSU
• 金额: $ 0.8万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721453
• 关键词: Anions; Cardiolipins; Complex; Computer Retrieval of Information on Scientific Projects Database; Dissociation; Fatty Acids; Funding; Glycerol; Grant; Institution; Ions; Isomerism; Methods; Molecular Structure; Phosphatidic Acid; Phosphatidyl glycerol; Phosphatidylglycerols; Phospholipids; Positioning Attribute; Reporting; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Vertebral column; instrument; lead ion; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We report negative-ion electrospray tandem mass spectrometric methods for structural characterization of cardiolipin (CL), a four-acyl-chain phospholipid containing two distinct phosphatidyl moieties, of which structural assignment of the fatty acid residues attached to the glycerol backbones performed by low-energy CAD tandem mass spectrometry has not been previously described. The low-energy MS2-spectra of the [M - H]- and [M - 2H]2- ions obtained with ion-trap or with tandem quadrupole instrument combined with ion-trap MS3-spectra or with source CAD product-ion spectra provide complete structural information for CL characterization. The MS2-spectra of the [M - H]- ions contain two sets of prominent fragment ions that comprise a phosphatidic acid, a dehydrated phosphatidylglycerol, and a (phosphatidic acid + 136) anion. The substantial differences in the abundances of the two distinct phosphatidic anions observed in the MS2-spectra of the [M - H]- ions lead to the assignment of the phosphatidyl moieties attached to the 1' or 3' position of central glycerol. Upon further collisional dissociation, the MS3-spectra of the phosphatidic anions provide information to identify the fatty acyl substituents and their position in the glycerol backbone. The MS2-spectra of the [M - 2H]2- ions obtained with TSQ or ITMS contain complementary information to confirm structural assignment. The applications of the above methods in the differentiation of cardiolipin isomers and in the identification of complex cardiolipin species consisting of multiple molecular structures are also demonstrated.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们报告了负极电喷雾串联质谱法，用于心霉素（CL）的结构表征，这是一种四酰基 - 链磷脂，其中包含两个不同的磷脂基部分，其中脂肪酸残基的结构分配附着在低含量上的甘油骨backbone backbone backbone by-Enerergy cad cad tandem spectorement曾经描述过。 [M-H]和[M-H]和[M-2H] 2-离子的低能量MS2-Spectra与离子陷阱或串联四极杆仪器与离子陷阱MS3-Spectra或源CAD CAD产品-ION光谱相结合提供了完整的结构信息，以提供CL表征的完整结构信息。 [M-H] - 离子的MS2-spectra包含两组突出的片段离子，这些碎片离子包含磷脂酸，脱水的磷脂酰甘油和A（磷酸 + 136）阴离子。在[m-h] - 离子的MS2-spectra中观察到的两个不同磷酸阴离子的丰度的实质差异导致分配中央甘油的1'或3'位置的磷脂酰部分。在进一步的碰撞解离后，磷脂阴离子的MS3谱线提供了信息，以识别脂肪酰基取代基及其在甘油主链中的位置。使用TSQ或ITM获得的[M-2H] 2离子的MS2-Spectra包含补充信息以确认结构分配。上述方法在分化心脂蛋白异构体和鉴定由多个分子结构组成的复杂心脂蛋白物种中的应用。