NMSU CELL AND ORGANISM CORE FACILITY

NMSU 细胞和生物体核心设施

基本信息

批准号：
7720449
负责人：
Graciela Alexandra Unguez
金额：
$ 9.46万
依托单位：
NEW MEXICO STATE UNIVERSITY LAS CRUCES
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-05-01 至 2009-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7720449
关键词：
Animals Binding Proteins Biochemistry Biology Cell Culture Techniques Cell Cycle Regulation Cell physiology Cells Chemistry Communities Computer Retrieval of Information on Scientific Projects Database Core Facility Cytokinesis Daily Faculty Funding Goals Grant In Vitro Institution Life Mammalian Cell Molecular Muscle Natural regeneration New Mexico Organism Pilot Projects Preparation Primary Cell Cultures Protocols documentation Range Research Research Activity Research Infrastructure Research Personnel Research Project Grants Resources Scientist Source Students Support of Research Time Tissues Training United States National Institutes of Health Universities Vertebrates Visit cell dedifferentiation embryonic stem cell established cell line instrumentation programs protein protein interaction research study satellite cell teleost tissue culture transdifferentiation

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell and Organism Core Facility (COCF) at New Mexico State University provides a proper facility equipped with instrumentation to carry out tissue culture experiments using funds from the New Mexico BRIN/INBRE grant. This facility is a critical asset to the research programs of junior faculty in the Biology and Chemistry/Biochemistry Departments at NMSU who use in vitro approaches in their studies and some junior faculty who also receive INBRE funding through pilot projects. Hence, the function of the COCF meets one of the stated goals of the New Mexico INBRE: to create a research infrastructure for investigators and students to perform studies using primary cell cultures, live tissue explant preparations from a variety of animal organisms, and established cell lines. In addition, the COCF supports research activities that benefit non-INBRE researchers at NMSU and promotes its use by non-university scientists in the community. The COCF currently supports research activities that involve students, staff and faculty and focus on 5 active research projects. These include: 1) molecular mechanisms involved in the timing of cytokinesis and cell cycle regulation (Dr. Charles Shuster) 2) cell dedifferentiation and transdifferentiation during regeneration in vertebrates (Dr. Graciela Unguez), 3) functional characterization of teleost muscle regulatory factors in mammalian embryonic stem cells and their myogenic conversion capacity (Dr. Graciela Unguez), 4) establishment of primary satellite cell culture from S. macrurus (Dr. Graciela Unguez), and 5) protein-protein interaction of Grb7 and its candidate binding proteins in mammalian cells (Dr. Barbara Lyons). The COCF is enabling daily research activities involving not only faculty, but their graduate and undergraduate students as well. In the past 10 months, a total of thirteen students (7 graduate, 6 undergraduate) have been trained in cell culture techniques and have developed protocols optimized for tissue-specific in vitro studies. Further maturation of the facility will allow for a broader range of research and instructional projects and support the needs of additional NMSU researchers and visiting scientists.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此，可以在其他清晰的条目中表示。列出的机构是对于中心，这不一定是调查员的机构。新墨西哥州立大学的细胞和生物核心设施（COCF）提供了配备仪器的适当设施，使用新墨西哥州Brin/Inbre Grant的资金进行组织培养实验。该设施是NMSU生物学/化学/生物化学部门初级教师研究计划的重要资产，他们在研究中使用体外方法，以及一些通过试点项目获得Inbre资金的初级教师。因此，COCF的功能符合新墨西哥州inbre的既定目标之一：为研究人员和学生创建研究基础设施，以使用原始细胞培养物进行研究，来自各种动物生物体的实时组织外植体制备，并建立了细胞的细胞。线。此外，COCF还支持使NMSU中非迅速研究人员受益的研究活动，并促进了社区中非大学科学家的使用。 COCF目前支持涉及学生，教职员工和教职员工的研究活动，并专注于5个活跃的研究项目。其中包括：1）参与细胞因子和细胞周期调节时间的分子机制（Charles Shuster博士）2）脊椎动物的再生（Graciela unguez博士），3）细胞去分化和转分化的功能。哺乳动物的胚胎干细胞及其肌源性转化能力（Graciela Unguez博士），4）从S. macrurus（Graciela unguez博士）和5）GRB7的蛋白质蛋白质相互作用及其候选蛋白结合在Macrurus（Graciela unguez博士）和5）中的原代卫星细胞培养哺乳动物细胞（Barbara Lyons博士）。 COCF正在实现每日研究活动，不仅涉及教职员工，而且还可以研究他们的毕业生和本科生。在过去的10个月中，总共有13名学生（7名毕业生，6名本科）接受了细胞培养技术的培训，并开发了针对组织特异性研究的优化方案。该设施的进一步成熟将允许更广泛的研究和教学项目，并支持其他NMSU研究人员和来访科学家的需求。