NMSU CELL AND ORGANISM CORE FACILITY

NMSU 细胞和生物体核心设施

• 批准号: 7960224
• 负责人: Graciela Alexandra Unguez
• 金额: $ 9.65万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960224
• 关键词: Biomedical Research; Cell Culture Techniques; Cell Cycle Regulation; Cells; Communities; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Cytokinesis; Development; Faculty; Funding; Grant; In Vitro; Institution; Life; Molecular; Natural regeneration; New Mexico; Organism; Preparation; Primary Cell Cultures; Protocols documentation; Research; Research Activity; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Scientist; Source; Students; Time; Tissues; Training; Transfection; United States National Institutes of Health; Universities; Vertebrates; Visit; cell dedifferentiation; established cell line; programs; research study; satellite cell; teleost; tissue culture; transdifferentiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell and Organism Core Facility (COCF) at New Mexico State University provides a facility equipped to carry out tissue culture experiments using funds from the NM BRIN/INBRE grant. It is a critical asset to the research programs of INBRE and non-INBRE funded junior faculty by creating a research infrastructure for investigators and students to perform studies using primary cell cultures, live tissue explant preparations from a variety of organisms, and established cell lines. The COCF also promotes its use by non-university scientists in the community. The COCF currently supports 4 active research projects that involve students, staff and faculty. These include: 1) molecular mechanisms involved in the timing of cytokinesis and cell cycle regulation (Shuster) 2) cell dedifferentiation and transdifferentiation during regeneration in vertebrates (Unguez), 3) establishment of primary satellite cell culture from a teleost vertebrate S. macrurus (Unguez), and 4) development of new mechanisms of intracellular transfection (Smirnov). The COCF is enabling daily research activities involving not only faculty, but their graduate and undergraduate students as well. In the past 12 months, a total of twelve students (6 graduate, 6 undergraduate) were trained in cell culture techniques and these students developed protocols optimized for tissue-specific in vitro studies. Further maturation of the facility will incorporate a broader range of projects and support the needs of additional NMSU researchers and visiting scientists.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 新墨西哥州立大学的细胞和生物体核心设施 (COCF) 提供了一个利用 NM BRIN/INBRE 拨款进行组织培养实验的设施。 它为研究人员和学生创建研究基础设施，使用原代细胞培养物、来自各种生物体的活组织外植体制剂和已建立的细胞系进行研究，是 INBRE 和非 INBRE 资助的初级教师研究项目的重要资产。 COCF 还促进社区中的非大学科学家使用它。 COCF 目前支持 4 个活跃的研究项目，涉及学生、教职员工和教师。 这些包括：1) 参与胞质分裂和细胞周期调节的分子机制 (Shuster) 2) 脊椎动物再生过程中的细胞去分化和转分化 (Unguez)，3) 从硬骨鱼脊椎动物 S. macrurus 建立原代卫星细胞培养物 ( Unguez），4）细胞内转染新机制的开发（Smirnov）。 COCF 不仅支持教师的日常研究活动，还支持其研究生和本科生的参与。 在过去 12 个月中，共有 12 名学生（6 名研究生，6 名本科生）接受了细胞培养技术培训，这些学生开发了针对组织特异性体外研究优化的方案。 该设施的进一步成熟将纳入更广泛的项目，并支持更多新墨西哥州立大学研究人员和访问科学家的需求。