DNA BARCODING AND IDENTIFICATION OF BLACK HILLS ARACHNIDS

黑山蜘蛛的 DNA 条形码和鉴定

• 批准号: 7720211
• 负责人: SHANE K SARVER
• 金额: $ 5.74万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720211
• 关键词: Arachnida; Collection; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Databases; Funding; Genbank; Genes; Genetic; Grant; Institution; Methods; Mitochondria; Museums; Research; Research Personnel; Resources; Sampling; Source; South Dakota; Specimen; Surveys; Taxonomy; Training; United States National Institutes of Health; cytochrome c oxidase; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Virtually nothing is known about the arachnid fauna of the Black Hills region of South Dakota. Traditional identification of arachnids requires a trained systematist and taxonomic keys for a given region. Since neither of these is presently available for the Black Hills of South Dakota, a survey of the arachnid fauna in the Black Hills is virtually impossible. A different approach is being used for the present project, that could best be described as "reverse taxonomy." Arachnid samples from the Black Hills are being collected and then sampled for DNA and characterized for a region of the cytochrome oxidase I mitochondrial gene. These DNA barcodes will then be compared with vouchered specimens obtained either from DNA databases, such as genbank or with specimens obtained in collections from museums. This DNA barcoding approach not only provides a method of taxonomic identification, but also affords the opportunity to identify new species or novel genetic forms of described species.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 几乎没有关于南达科他州黑山地区的蛛网动物区系知之甚少。蛛网的传统识别需要给定区域的训练有素的系统主义和分类键。由于目前都不适用于南达科他州的黑山，因此几乎不可能对黑山的蛛网动物群进行调查。目前的项目正在使用另一种方法，最好被描述为“反向分类法”。正在收集来自黑山的蛛网样品，然后采样以DNA为单位，并为细胞色素氧化酶I线粒体基因的区域进行表征。然后，将将这些DNA条形码与从GenBank等DNA数据库中获得的保证标本或从博物馆收藏中获得的标本进行比较。这种DNA条形码方法不仅提供了一种分类学识别方法，而且还提供了鉴定新物种或新颖的描述物种的新遗传形式的机会。