MICROSIMULATION OF ANTHRACIS-IMMUNE SYSTEM INTERACTION - ADJUVANT DEVELOPMENT

炭疽病-免疫系统相互作用的微观模拟 - 佐剂开发

• 批准号: 8172397
• 负责人: Thomas B Kepler
• 金额: $ 4.39万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172397
• 关键词: Adjuvant; Antigen Presentation; Apoptosis; Bacillus anthracis; Back; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Data Collection; Dermal; Development; Experimental Designs; Funding; Grant; Immune response; Immune system; Immunization; Institution; Interleukin-12; Lymphoid; Mediating; Modeling; Mus; Myelogenous; Process; Production; Research; Research Personnel; Resources; Source; United States National Institutes of Health; design; in vivo; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project was initiated to design and implement in vivo experiments to analyze immune responses in vivo in mice, within a systematically integrated process that cycles from experimental design, to data collection, to data analysis, to parameter estimation, to goodness of fit determination, to deduction from model, and back to experimental design. The data generated thus far suggest: 1) That distinct subsets of DCs sequentially migrate to the draining LNs after immunization with OVA+LPS. The caveat to this is that the change in DC numbers observed may instead reflect differences in differentiation, proliferation or apoptosis. Experiments to clarify these issues are in progress. 2) That IL-12 production is mediated predominantly by Lymphoid DCs and Langerhans DC, but by myeloid DCs or dermal DCs, suggesting a division of labor amongst the DC subsets. 3) That antigen-presentation is mediated by all DC subsets.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目旨在设计和实施体内实验，以分析小鼠体内的免疫反应，在系统整合的过程中，该过程从实验设计到数据收集到数据分析，到参数估计，到拟合确定的好处，从模型和回溯到实验设计。 迄今为止生成的数据表明： 1）DC的不同子集用OVA+LPS免疫后，依次迁移到排水LN。警告是，观察到的直流数的变化可能反映出分化，增殖或凋亡的差异。阐明这些问题的实验正在进行中。 2）IL-12的产生主要由淋巴样DC和Langerhans DC介导，但由髓样DC或真皮DC介导，这表明DC子集中有劳动力分裂。 3）所有DC子集介导了抗原呈递。