CORE--MOLECULAR GENETICS

核心--分子遗传学

• 批准号: 7010662
• 负责人: MARK LEPPERT
• 金额: $ 18.21万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7010662
• 关键词: biomedical facility; familial hypertension; genetic markers; molecular genetics

DESCRIPTION: (Adapted from the applicant's abstract) Genotypes for linkage and association were carried out in support of investigators in the current SCOR. A total of 41 separate marker systems from 21 genes were run. In all, 6,647 genotypes were obtained for this study. As new samples were arriving at the Core throughout the course of the grant, not all of the samples have been typed for all marker systems, however, an effort was made to complete typing on the new samples for key marker systems that yielded promising results in the original analysis. The Molecular Genetics core currently is completing the genotyping on the last group of a total of 214 sibling pair samples and for association studies and an additional 120 singleton samples for a total of 434 DNA samples. Linkage markers close to candidate genes as well as single nucleotide polymorphism (SNPs) within the candidate genes were run. Linkage markers were carried out using a radioactive/acrylamide detection system. SNPs were detected with either mass spectrometry, the allelic discrimination system of the Perkin-Elmer ABI Prism 7700, or restriction enzyme digestion followed by resolution on agarose gels.

描述：（改编自申请人的摘要）连锁和关联的基因型是为了支持当前 SCOR 中的研究人员而进行的。 总共运行了来自 21 个基因的 41 个独立标记系统。 这项研究总共获得了 6,647 个基因型。 由于在整个资助过程中新样本不断到达核心，因此并非所有样本都已针对所有标记系统进行了分型，但是，我们已努力完成关键标记系统的新样本分型，这在以下方面取得了有希望的结果：原来的分析。 分子遗传学核心目前正在完成最后一组总共 214 个兄弟姐妹样本的基因分型，用于关联研究，以及另外 120 个单例样本，总共 434 个 DNA 样本。 运行靠近候选基因的连锁标记以及候选基因内的单核苷酸多态性（SNP）。 使用放射性/丙烯酰胺检测系统进行连锁标记。 SNP 可通过质谱、Perkin-Elmer ABI Prism 7700 的等位基因鉴别系统或限制酶消化并在琼脂糖凝胶上解析来检测。