CORE--MOLECULAR GENOTYPING

核心——分子基因分型

• 批准号: 6302404
• 负责人: Val C. Sheffield
• 金额: $ 19.94万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2001-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6302404
• 关键词: biomedical facility; familial hypertension; genetic mapping; genetic markers; genotype; molecular genetics; nucleic acid repetitive sequence

Hpertension is a complex genetic trait which is thought to involve the simultaneous presence of mutations in multiple genes. The identification of genes involved in hypertension will aid in the understanding of hypertension pathophysiology, allow for the presymptomatic diagnosis of high risk individuals, and potentially lead to novel forms of treatments. The major goal of this Hypertension SCOR is to use molecular methods to identify genetic loci contributing to this complex disorder. The most useful methods available for genetic dissection of complex diseases include nonparametric allele-sharing analysis of related affected human subjects (sib-pair and affected pedigree member analysis), and genetic analysis of animal models. Projects utilizing these methods are a major part of this Hypertension SCOR. This project will contribute to the identification of loci involved in hypertension by providing the necessary molecular resources for projects 1, 2 and 4. Specifically, we will use state-of-the-art methods to provide high throughput genotyping for both human and rat studies. In addition, we will contribute to the construction of a dense genetic map of the rat genome, by developing a minimum of 1000 high quality short tandem repeat polymorphic markers (STRPs). These markers will be made available to the scientific community, thus facilitating the study of rat models of hypertension in this and other Hypertension SCORs.

Hpertension是一个复杂的遗传特征，被认为涉及 在多个基因中同时存在突变。标识 高血压涉及的基因将有助于理解 高血压病理生理学，允许对 高风险个体，并有可能导致新的治疗形式。 该高血压SCOR的主要目标是使用分子方法 确定遗传基因座有助于这种复杂疾病。最多 可用于复杂疾病的遗传解剖的有用方法 包括对相关受影响人类的非参数等位基因共享分析 受试者（SIB-PAIR和受影响的谱系成员分析）和遗传 动物模型的分析。利用这些方法的项目是主要的 该高血压得分的一部分。该项目将有助于 通过提供必要的 项目1、2和4的分子资源。具体来说，我们将使用 为两者提供高通量基因分型的最新方法 人与大鼠研究。此外，我们将为建设做出贡献 大鼠基因组的致密遗传图，通过开发至少1000 高质量的短串联重复多态标记（Strps）。这些 标记将提供给科学界，因此 促进该和其他高血压大鼠模型的研究 高血压SCOR。