CHARACTERIZATION OF ZYGOTE ARREST ONE (ZAR1) IN NONHUMAN PRIMATES

非人类灵长类动物受精卵逮捕一 (ZAR1) 的特征

• 批准号: 7561935
• 负责人: Xuemei Wu
• 金额: $ 1.47万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561935
• 关键词: Antibodies; Complementary DNA; Computer Retrieval of Information on Scientific Projects Database; Development; Embryo; Embryonic Development; Evolution; Female; Fertility; Funding; Grant; Human; In Vitro; Infertility; Institution; Messenger RNA; Microarray Analysis; Molecular; Monkeys; Mus; Oocytes; Ovary; Pattern; Proteins; RNA Interference; Research; Research Personnel; Resources; Source; Staging; System; Techniques; Testis; Transgenic Organisms; United States National Institutes of Health; nonhuman primate; protein expression; zygote

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ZAR1 is a newly identified oocyte-expressed protein conserved during evolution. Female mice lacking ZAR1 are infertile due to a block of embryogenesis at the zygote stage. Thus, ZAR1 belongs to a small group of maternal effect proteins that are required for the oocyte-to-embryo transition in mice. In humans, however, ZAR1 is detectable in both the ovary and the testis. To elucidate functions of ZAR1 in human fertility, we chose nonhuman primates as our mammalian system due to their close resemblance to humans in development. The aim of this project is to demonstrate potential functions of ZAR1 during early embryogenesis and identify key molecules that function at the oocyte-to-embryo transition in monkeys. We have discovered a monkey cDNA that is highly homologous to mouse and human ZAR1, and determined the expression pattern of monkey ZAR1 mRNA. The specific aims are as follows: (1) to characterize ZAR1 protein expression in monkeys. Specific antibodies that recognize a highly conserved region of ZAR1 protein will be generated and used to determine ZAR1 expression in monkeys and humans; (2) to demonstrate potential functions of ZAR1 during early monkey embryo development. In vitro RNAi and transgenic techniques will be employed in monkey oocytes and early embryos; (3) to identify key molecules that function at the oocyte-to-embryo transition in nonhuman primates. We will perform microarray analysis to identify differentially expressed molecules in ZAR1 knockdown embryos. The study described here is among the first attempts to illustrate molecular mechanisms that regulate preimplantation embryogenesis in nonhuman primates.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 ZAR1是在进化过程中保守的新鉴定的卵母细胞表达的蛋白质。缺乏ZAR1的雌性小鼠由于在合子阶段的胚胎发生而不育。 因此，Zar1属于小鼠卵母细胞到胚胎过渡所需的一小部分母体作用蛋白。 然而，在人类中，Zar1在卵巢和睾丸中均可检测到。 为了阐明ZAR1在人类生育中的功能，我们选择了非人类灵长类动物作为我们的哺乳动物系统，因为它们与人类的发展非常相似。 该项目的目的是在早期胚胎发生过程中证明ZAR1的潜在功能，并确定在猴子中卵母细胞到胚胎过渡的关键分子。 我们发现了一种与小鼠和人Zar1高度同源的猴子cDNA，并确定了猴子ZAR1 mRNA的表达模式。 具体目的如下：（1）表征猴子中ZAR1蛋白的表达。 识别高度保守区域的ZAR1蛋白区域的特定抗体将产生并用于确定猴子和人类中的Zar1表达。 （2）在早期猴子胚胎发育过程中展示ZAR1的潜在功能。 体外RNAi和转基因技术将用于猴子卵母细胞和早期胚胎。 （3）确定在非人类灵长类动物中卵母细胞到胚胎过渡的关键分子。 我们将进行微阵列分析，以鉴定ZAR1敲低胚胎中差异表达的分子。 此处描述的研究是说明调节非人类灵长类动物植入前胚胎发生的分子机制的首次尝试。