WNPRC GENETICS SERVICES UNIT

WNPRC 遗传学服务单位

• 批准号: 8173098
• 负责人: David H. O'Connor
• 金额: $ 10.33万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173098
• 关键词: AIDS Vaccines; Acquired Immunodeficiency Syndrome; Acute; Alleles; Allogenic; Ascaridil; Autologous; Base Sequence; Biological Assay; Breeding; CD8B1 gene; Cercocebus atys; Cercopithecus pygerythrus; Chinese People; Chronic; Cloning; Communicable Diseases; Complementary DNA; Computer Retrieval of Information on Scientific Projects Database; DNA; Detection; Educational workshop; Family suidae; Fee-for-Service Plans; Filipino; Funding; Genetic; Genetic Services; Genome; Genomics; Genotype; Grant; Immune; Immunogenetics; Infection; Institutes; Institution; International AIDS; Japan; Japanese Population; Laboratories; Lead; Length; Lymphoid Tissue; MHC Class I Genes; Macaca; Macaca mulatta; Major Histocompatibility Complex; Manuscripts; Methods; Microsatellite Repeats; Modeling; National Center for Research Resources; National Institute of Allergy and Infectious Disease; Price; Primates; Printing; Publications; RNA; Research; Research Personnel; Resolution; Resource Allocation; Resources; SIV; Sampling; Services; Source; T-Lymphocyte; Tail; Technology; Training; United States National Institutes of Health; Vaccinated; Vaccine Research; Variant; Virus; animal resource; base; bean; cohort; cost effective; genetic pedigree; genetic resource; interest; lectures; meetings; member; next generation; nonhuman primate; novel; pathogen; programs; repository; symposium; trafficking; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To provide core, campus and non-host investigators with sophisticated and specialized genetics resources and expertise. Progress and concerns: In 2009 Genetics Services continued to aggressively develop new high resolution MHC genotyping in rhesus, cynomolgus and pig-tailed macaques using next generation sequencing technology. In addition, we have begun to expand our efforts to other nonhuman primates of experimental interest such as sooty mangabeys, African green monkeys and Japanese macaques. Microsatellite-based haplotyping has been used extensively for genotyping of Mauritian-origin cynomolgus macaques as well as sample authentication and pedigree verification. Specialized MHC genotyping services are also available for Indian- and Chinese-origin rhesus macaques, pig-tailed macaques, and cynomolgus macaques from various geographic origins. Genetics Services is taking the lead in developing high-resolution, sensitive, and economical assays for comprehensive MHC genotyping in macaques. During 2009, this included a major expansion of our fee-for-service program for high throughput, sequence-based MHC class I genotyping using Roche/454 next-generation platforms for external investigators. We are currently genotyping NHP cohorts from simian immune deficiency virus studies as well as characterizing breeding colonies. Allocation of resource access: The Genetics Service provided MHC genotyping support to more than two dozen laboratories around the world in 2009. In addition, we participated in the NCRR Primate Centers Working Group on Genetics and Genomics, as well as the Genome Banking Working Group that has generated a large repository of genomic DNA samples from various nonhuman primates including WNPRC rhesus and cynomolgus macaques. We are also contributing our expertise as members of the Working Group for the Primate Portal. Dissemination: Dr. O'Connor, the PI for Genetics Services, and Dr. Wiseman presented multiple lectures and authored nine publications on NHP genetics (see below). We request that projects utilizing Genetics Service acknowledge the service in manuscripts and presentations. Training: Methods for MHC genotyping are regularly discussed during scientific conferences attended by Dr. O'Connor and his staff. In 2009, this included multiple presentations for the International AIDS Vaccine Initiative (IAVI) and at the NIH for NIAID, NCRR, DIADS & the Vaccine Research Program. In addition, lectures were given at the following scientific meetings: + Detection, Impact and Control of Specific Pathogens in Animal Resource Facilities Workshop sponsored by NCRR/NIA + Tsukuba Primate Research Center, National Institute of Infectious Diseases, Japan + 27th Annual Symposium for Nonhuman Primate Models for AIDS PUBLICATIONS: Campbell KJ, Detmer AM, Karl JA, Wiseman RW, Blasky AJ, Hughes AL, Bimber BN, O'Connor SL, O'Connor DH. Characterization of 47 MHC class I sequences in Filipino cynomolgus macaques. Immunogenetics. 2009 61(3): 177-87. Burwitz BJ, Pendley CJ, Greene JM, Detmer AM, Lhost JJ, Karl JA, Piaskowski SM, Rudersdorf RA, Wallace LT, Bimber BN, Loffredo JT, Cox DG, Bardet W, Hildebrand W, Wiseman RW, O'Connor SL, O'Connor DH. Mauritian cynomolgus macaques share two exceptionally common major histocompatibility complex class I alleles that restrict simian immunodeficiency virus-specific CD8+ T cells. J Virol. 2009 83(12): 6011-9. Karl JA, Wiseman RW, O'Connor DH. Cost-effective sequence-based nonhuman primate MHC class I genotyping from RNA. Methods. 2009 49(1): 11-7. Wiseman RW, Karl JA, Bimber BN, O'Leary CE, Lank SM, Tuscher JJ, Detmer AM, Bouffard P, Levenkova N, Turcotte CL, Szekeres E Jr, Wright C, Harkins T, O'Connor DH. Major histocompatibility complex genotyping with massively parallel pyrosequencing. Nat Med. 2009 15(11): 1322-6. O'Leary CE, Wiseman RW, Karl JA, Bimber BN, Lank SM, Tuscher JJ, O'Connor DH. Identification of novel MHC class I sequences in pig-tailed macaques by amplicon pyrosequencing and full-length cDNA cloning and sequencing. Immunogenetics. 2009 61(10): 689-701. Valentine LE, Loffredo JT, Bean AT, Le¿n EJ, MacNair CE, Beal DR, Piaskowski SM, Klimentidis YC, Lank SM, Wiseman RW, Weinfurter JT, May GE, Rakasz EG, Wilson NA, Friedrich TC, O'Connor DH, Allison DB, Watkins DI. Infection with "escaped" virus variants impairs control of simian immunodeficiency virus SIVmac239 replication in Mamu-B*08-positive macaques. J Virol. 2009 83(22): 11514-27. Kanthaswamy S, Capitanio JP, Dubay CJ, Ferguson B, Folks T, Ha JC, Hotchkiss CE, Johnson ZP, Katze MG, Kean LS, Kubisch HM, Lank S, Lyons LA, Miller GM, Nylander J, O'Connor DH, Palermo RE, Smith DG, Vallender EJ, Wiseman RW, Rogers J. Resources for genetic management and genomics research on non-human primates at the National Primate Research Centers (NPRCs). J Med Primatol. 2009 38 Suppl 1: 17-23. Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M Jr, O'Connor D, Lifson JD, Roederer M, Ohlen C. Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques. J Immunol. 2010 184(1): 303-14. Greene JM, Lhost JJ, Burwitz BJ, Budde ML, Macnair CE, Weiker MK, Gostick E, Friedrich TC, Broman KW, Price DA, O'Connor S, O'Connor DH. Extra-lymphoid tissue-resident CD8+ T cells from SIVmac239Deltanef-vaccinated macaques suppress SIVmac239 replication ex vivo. J Virol. 2010 Jan 20.[Epub ahead of print].

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 目的：为核心，校园和非主持人调查人员提供精致和专业的遗传资源和专业知识。 进步和关注： 2009年，遗传学服务继续积极发展新的高分辨率MHC基因分型，使用下一代测序技术，在恒河猴，cynomolgus和Pig-Tailed猕猴中进行了基因分型。此外，我们已经开始将我们的精力扩展到其他非人类的实验兴趣隐私，例如烟草，非洲绿猴和日本猕猴。基于微卫星的单倍型已广泛用于毛丽丽 - 原菌素cynomolgus猕猴的基因分型以及样品认证和谱系验证。专门的MHC基因分型服务也可用于印度和中文恒河猕猴，猪尾猕猴以及来自各种地理起源的Cynomolgus猕猴。遗传学服务正在促进猕猴中全面的MHC基因分型开发高分辨率，敏感和经济测定法。在2009年期间，这包括我们使用Roche/454下一代研究人员的高吞吐量，基于序列的MHC I类基因分型的费用服务计划的重大扩展。我们目前正在基因分型NHP队列中的NHP人群研究，并表征繁殖菌落。 资源访问的分配： 遗传学服务在2009年为世界各地的两十多名实验室提供了MHC基因分型支持。此外，我们参加了NCRR灵长类动物中心的遗传和基因组学工作组以及基因组银行工作组，以及来自包括WnprC和Cynprc ser的基因组DNA样本的基因组库存工作组。作为灵长类动物门户工作组成员，我们还在为我们的专业知识做出贡献。 传播： O'Connor博士，遗传学服务的PI，Wiseman博士发表了多个讲座，并撰写了九本关于NHP遗传学的出版物（见下文）。我们要求使用遗传学服务的项目确认手稿和演示文稿中的服务。 训练： 在O'Connor博士及其工作人员参加的科学会议期间，定期讨论MHC基因分型的方法。 2009年，这包括国际艾滋病疫苗倡议（IAVI）的多次演讲，以及NIH的NIAID，NCRR，DIADS和疫苗研究计划。此外，在以下科学会议上进行了讲座： + NCRR/NIA赞助的动物资源设施中的特定病原体的检测，影响和控制 +日本国家传染病研究所的Tsukuba灵长类研究中心 +非人类灵长类动物模型的第27届年度研讨会 出版物： Campbell KJ，Detmer AM，Karl JA，Wiseman RW，Blasky AJ，Hughes AL，Bimber BN，O'Connor SL，O'Connor DH。菲律宾cynomolgus猕猴中47个MHC I类序列的表征。免疫遗传学。 2009 61（3）：177-87。 Burwitz BJ，Pendley CJ，Greene JM，Detmer AM，Lho​​st JJ，Karl JA，Piaskowski SM，Rudersdorf RA，Wallace LT，Bimber BN，Bimber BN，Loffredo JT，Cox DG，Cox DG，Bardet W，Bardet W，Bardet W，Hildebrand W，Hildebrand W，Wiseman RW，Wiseman RW，O'Connor Sl，O'Conconnor，O'Connor，O'Connor dh。 Mauritian cynomolgus猕猴共享两个异常常见的主要组织相容性复合物I类I类等位基因，它们限制了SIMIAN免疫缺陷病毒特异性CD8+ T细胞。 J Virol。 2009 83（12）：6011-9。 Karl JA，Wiseman RW，O'Connor DH。基于成本效益的基于RNA的基于序列的非人类灵长类动物MHC I类I类基因分型。方法。 2009 49（1）：11-7。 Wiseman RW，Karl JA，Bimber BN，O'Leary CE，Lank SM，Tuscher JJ，Detmer AM，Bouffard P，Levenkova N，Turcotte CL，Szekeres E Jr，Wright C，Harkins C，Harkins T，O'Connor DH。主要的组织相容性复合基因分型与大量平行的焦磷酸测序。 Nat Med。 2009 15（11）：1322-6。 O'Leary CE，Wiseman RW，Karl JA，Bimber BN，Lank SM，Tuscher JJ，O'Connor DH。通过Amplicon pyrosequencing和全长cDNA克隆和测序鉴定猪尾猕猴中新型MHC I类序列的鉴定。免疫遗传学。 2009 61（10）：689-701。 Valentine LE，Loffredo JT，Bean AT，Le¿n EJ，Macnair CE，Beal DR，Piaskowski SM，Klimentdis YC，Lank SM，Wiseman RW，Weinfurter JT，May GE，Rakasz EG，Rakasz EG，Wilson Na，Wilson Na，Friedrich TC，Friedrich TC，O'Connor DH，O'Connor DH，Allison DB，WALSISON DI。用“逃脱”病毒变体的感染会损害MAMU-B*08阳性猕猴中猿猴免疫缺陷病毒SIVMAC239复制的控制。 J Virol。 2009 83（22）：11514-27。 Kanthaswamy S，Capitanio JP，Dubay CJ，Ferguson B，Folks T，Ha JC，Hah JC，Hotchkiss CE，Johnson ZP，Katze MG，Kean LS，Kean LS，Kubisch HM，Lank S，Lank S，Lyons LANK S，Lyons LA，Lyons LA，Miller GM，Nylander J，Nylander J，Nylander J，O'Conconnor dh，palermo rederner J.用于国家灵长类动物研究中心（NPRC）的非人类灵长类动物的遗传管理和基因组学研究。 J Med Primatol。 2009 38补充1：17-23。 Bolton DL，Minang JT，Trivett MT，Song K，Tuscher JJ，Li Y，Piatak M Jr，O'Connor D，O'Connor D，Lifson JD，Roederer M，Ohlen C.运输，持久性，持久性，持久性和激活状态适当地转移了同种异体和自动性的临床及其自动性含量的CD8（+）ccd8（+）ccd8，+）猕猴。 J免疫。 2010 184（1）：303-14。 Greene JM，Lho​​st JJ，Burwitz BJ，Budde ML，Macnair CE，Weiker MK，Gostick E，Friedrich TC，Broman KW，Price DA，O'Connor S，O'Connor S，O'Connor DH。来自SIVMAC239DELTANEF接种误解的猕猴的淋巴外组织居住的CD8+ T细胞抑制了SIVMAC239复制。 J Virol。 2010年1月20日。