ROLE OF MHC CLASS I ALLELES IN VIRAL CONTROL OF SIV IN SOOTY MANGABEYS

MHC I 类等位基因在乌白眉猴 SIV 病毒控制中的作用

• 批准号: 8172408
• 负责人: Amitinder Kaur
• 金额: $ 4.39万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172408
• 关键词: Acquired Immunodeficiency Syndrome; Alleles; CD8-Positive T-Lymphocytes; Cercocebus atys; Complementary DNA; Computer Retrieval of Information on Scientific Projects Database; Cytotoxic T-Lymphocytes; DNA; Exons; Funding; Genomics; Genotype; Grant; HIV; Institution; Length; MHC Class I Genes; Oligonucleotide Probes; Pathogenesis; Play; Primates; Research; Research Personnel; Resources; Role; SIV; Source; Study models; T-Lymphocyte Epitopes; United States National Institutes of Health; Viral; Virus Diseases; Virus Replication; base; cDNA Library; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. MHC class I-restricted cytotoxic T-lymphocytes (CTL) play an important role in controlling human immunodeficiency virus and simian immunodeficiency virus (SIV) infection. As a natural host of SIV, sooty mangabeys (Cercocebus atys; Ceat) are a valuable model for the study of AIDS pathogenesis. We have previously defined several immunodominant SIV-specific CTL epitopes in sooty mangabeys and shown that CD8+ T lymphocytes inhibit SIV replication in vivo in naturally SIV-infected sooty mangabeys. In order to identify the MHC class I restriction of immunodominant CTL epitopes, we have cloned and characterized MHC class I genes of sooty mangabeys. cDNA libraries generated from two naturally SIV-infected sooty mangabeys were screened for MHC Class I cDNA with an oligonucleotide probe which hybridizes to a conserved region in exon 4 of primate MHC Class I genes. Based on a minimum of two clones with identical full-length MHC class I sequence, to date we have identified five MHC-A locus alleles designated as Ceat-A*0101 to Ceat-A*0501, and 12 MHC-B locus alleles designated as Ceat-B*0101 to Ceat-B*1201 in sooty mangabeys. Sequence specific primers (SSP) have been developed for the defined MHC class I alleles to enable large-scale MHC Class I typing of sooty mangabey genomic DNA by PCR-SSP. MHC genotyping of the colony of SIV-infected and SIV-negative sooty mangabeys at YNPRC is in progress.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 MHC I类限制的细胞毒性T淋巴细胞（CTL）在控制人免疫缺陷病毒和邻肌免疫缺陷病毒（SIV）感染中起着重要作用。作为SIV的天然寄主，烟熏芒果（Cercocebus atys; Ceat）是研究AIDS发病机理的宝贵模型。 我们以前已经定义了烟雾芒果中的几个免疫主导特异性CTL表位，并表明CD8+ T淋巴细胞抑制自然感染的SIV感染的烟粉杂种的体内SIV复制。为了识别MHC I类限制免疫主导CTL表位的限制，我们已经克隆并表征了MHC MHC I类烟草Mangabeys的基因。 用两个自然感染的SIV感染的烟灰芒果产生的cDNA文库被用寡核苷酸探针筛选了MHC I类cDNA，该探针与灵长类MHC I类基因的外显子4中的一个保守区域杂交。 基于至少两个具有相同全长MHC I类序列的克隆，迄今为止，我们已经确定了五个MHC-A基因座等位基因，称为Ceat-A*0101至Ceat-A*0501，而12 MHC-B基因座位数则指定为Ceat-b*0101 ceat-b*0101 ceat-b*1201 in sooty mangabeys。 已经为定义的MHC I类等位基因开发了序列特异性引物（SSP），以实现PCR-SSP的大规模MHC I类Sooty Mangabey基因组DNA。 YNPRC的SIV感染和SIV阴性烟草菌落的MHC基因分型正在进行中。