Functional Nanofibrous Scaffolds for Articular Cartilage Repair

用于关节软骨修复的功能性纳米纤维支架

基本信息

批准号：
7480824
负责人：
Chirakkal Krishnan
金额：
$ 20万
依托单位：
STONYBROOK TECHNOLOGY/APPLIED RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-04 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7480824
关键词：
Accounting Address Adoption Affect Affinity Air Anisotropy Applied Research Architecture Biocompatible Materials Biological Biological Products Biomedical Engineering Caliber Cartilage Cells Cellular Morphology Chemicals Chondrocytes Class Complex Condition Cytoskeletal Modeling Degenerative polyarthritis Engineering Environment Evaluation Excision Experimental Designs Extracellular Matrix Fiber Fibrinogen Fibroblast Growth Factor Fright Genes Goals Growth Factor Guidelines In Vitro Individual Insulin Kinetics Knowledge Laboratories Lead Maintenance Mechanics Medical Membrane Methods Molecular Monitor Morphology Nanotechnology Organic solvent product Oryctolagus cuniculus Pathway interactions Pattern Formation Performance Pharmaceutical Preparations Phase Phase II Clinical Trials Polymers Porosity Process Production Property Prostaglandins A Proteins Public Health Rate Research Retrieval Small Business Funding Mechanisms Small Business Innovation Research Grant Solutions Solvents Specialist Speed Stretching Structure Suspension substance Suspensions System Techniques Technology Testing Texture Thick Time Tissue Engineering Transforming Growth Factors United States National Institutes of Health Week aqueous articular cartilage base caprolactone cartilage regeneration cold temperature controlled release design desire dosage experience in vivo mRNA Expression nanofiber nanofilament polycaprolactone programs repaired scaffold tissue regeneration

项目摘要

DESCRIPTION (provided by applicant): The SBIR Phase I proposal aims to investigate the feasibility of developing a unique class of directed chondrocyte/poly(glycolide-co-caprolactone) (PGA-co-PCL) nanofibrous scaffold constructs for articular cartilage repair. Our hypothesis for the design and evaluation of this unique tissue regeneration system is based on several recent findings made in this and other laboratories. (1) Electrospun nanofibrous polycaprolactone (PCL)-based biodegradable scaffolds are suitable for maintenance of chondrocytes. (2) The unique nanofiber fabrication methods, i.e., multiple-jet electrospinning and electroblowing processes, developed by Stonybrook Technology and Applied Research (STAR), Inc., is ideal to fabricate highly porous 3D non-woven scaffolds on an industrial scale suitable for biomedical applications. (3) The multiple-jet electrospinning/electroblowing format in combination with coaxial spinning capability can allow the processing of delicate bioactive materials at low temperatures and in an aqueous environment, where growth factors can be incorporated without fear of thermal decomposition. (4) Post-processing approaches by mechanical stretching can be used to control the mechanical stability, anisotropy and the porosity of electrospun scaffolds. We propose that a judicious combination of the above technologies, i.e., 3D nanofibrous biodegradable scaffolds with prescribed degradation rate, mechanical stability, porosity, anisotropy and controlled-release capability of growth factors (i.e., fibroblast growth factor (FGF-2) and transforming growth factor-¿1 (TGF-¿1)) will offer a powerful pathway to prepare a new class of chondrocyte delivery scaffolds for repair of articular cartilage. PUBLIC HEALTH RELEVENCE: A unique class of chondrocyte/poly(glycolide-co-caprolactone) (PGA-co-PCL) nanofibrous scaffold constructs for articular cartilage repair is proposed. These constructs consist of three-dimensional biodegradable nanofibrous scaffolds with prescribed degradation rate, mechanical stability, porosity, anisotropy and controlled release capability of growth factors (i.e., fibroblast growth factor (FGF-2) and transforming growth factor-¿1 (TGF-¿1)).

描述（由适用提供）：SBIR I期提案旨在研究开发独特的有向软骨细胞/聚（Glycolide-co-caprolactone）（PGA-CO-PCL）（PGA-CO-PCL）纳米纤维支架构建体进行关节软骨修复的可行性。我们对这种独特的组织再生系统的设计和评估的假设是基于该实验室和其他实验室中的一些发现。（1）电纺纳米纤维多丙酮酸（PCL）的可生物降解脚手架适合维持软骨细胞。（2）由Stonybrook Technology and Applied Research（Star），Inc。开发的独特的纳米纤维制造方法，即多喷式静电纺丝和电吹制过程，非常适合在适用于生物医学应用的工业规模上制造高度多孔的3D 3D非织造脚手架。（3）多喷射电纺丝/电渗格格式与同轴旋转能力结合使用，可以在低温和水性环境中处理精致的生物活性材料，在这种环境中，可以在不担心热分解的情况下纳入生长因子。（4）通过机械拉伸的后处理方法可用于控制机械稳定性，各向异性和电纺支架的孔隙率。我们建议，上述技术的明智组合，即3D纳米纤维生物降解的脚手架，具有规定的降解速率，机械稳定性，机械稳定性，孔隙率，各向异性，各向异性和可控性生长因子的释放能力，即使成纤维细胞生长因子（FGF-2）和新的途径（FGF-2）和新的途径（FGF-2）提供了途径（FGF-2）。软骨细胞递送支架，用于修复关节软骨。公共卫生相关性：提出了一种独特的软骨细胞/聚乙二醇（乙二醇乙二醇）（PGA-CO-PCL）（PGA-CO-PCL）纳米纤维支架构建体，以用于关节软骨修复。这些构建体由具有规定的降解速率，机械稳定性，孔隙率，各向异性和受控释放能力的三维可生物降解纳米纤维支架组成。