A Bioinspired Small Diameter Vascular Conduit

仿生小直径血管导管

• 批准号: 7649262
• 负责人: Elliot Chaikof
• 金额: $ 77.73万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7649262
• 关键词: Animal Model; Arteries; Artificial Organs; Biochemical; Biological; Biomechanics; Biomimetic Materials; Biopolymers; Bioprosthesis device; Blood; Blood Vessel Prosthesis; Blood Vessels; Caliber; Cardiac; Cell physiology; Characteristics; Chemicals; Clinical; Clinical Research; Collagen; Collagen Type I; Development; Elastin; Elastin Fiber; Endothelial Cells; Engineering; Environment; Family; Fatigue; Fiber; Foundations; Generations; Genetic Techniques; Implant; In Vitro; Infiltration; Mechanics; Metabolic; Methodology; Molecular; Molecular Genetics; Molecular Structure; Operative Surgical Procedures; Papio; Performance; Physiological; Plastics; Porosity; Process; Property; Prosthesis; Proteins; Quaternary Protein Structure; Route; Scheme; Site; Smooth Muscle Myocytes; Solutions; Solvents; Structural Protein; Structure; Support System; Techniques; Tensile Strength; Testing; Tissues; Vascular Graft; analog; base; design; design and construction; implantation; in vivo; innovation; mathematical model; mechanical behavior; mimetics; monolayer; nanofabrication; resilience; response; scaffold; solid state; synthetic protein; tool

DESCRIPTION (provided by applicant): A clinically durable small diameter vascular graft may be achievable by optimizing the mechanical properties of an arterial bioprosthesis, as well as other biologically related characteristics. We believe that the molecular structure and supramolecular organization of native type I collagen and elastin fiber assembly establishes an important paradigm for the design of an arterial substitute composed of bio-inspired protein fiber analogues. Specifically, we intend to: (1) Determine the molecular level features of collagen and elastin fiber analogues that influence the mechanical behavior and physiochemical properties of protein-based fiber networks. Elastin and collagen analogues will be produced by biosynthetic and chemical schemes and processed into fiber networks by nanofabrication techniques. Both solution and solid state methodologies will be used to define material structure-property relationships. (2) Identify the micro scale characteristics of a protein fiber reinforced biopolymer composite that dictate mechanical responses relevant to the design of an arterial substitute. The mechanical behavior of both single and multicomponent fiber network composites will be investigated by static and dynamic mechanical testing under physiologically relevant conditions. In the process, constitutive mathematical models will be applied to assist in conduit design, as well as in the subsequent analysis of construct remodeling. (3) Define the morphological and structural remodeling of a collagen and elastin fiber reinforced vascular construct in vivo. Both acellular constructs, as well as conduits seeded with autogenous endothelial cells will be examined in a baboon animal model. Specifically, the effect of the local biological and mechanical environment on short- and long-term conduit properties, including patency and biostability will be defined. Lay summary: Optimizing mechanical and other biologically related properties may be an important step in the development of a small diameter arterial prosthesis critical to the fields of cardiac, plastic, and vascular surgery, as well as to the successful implantation of artificial organs and metabolic support systems.

描述（由申请人提供）：可以通过优化动脉生物繁殖发生的机械性能以及其他与生物学相关的特征来实现临床耐用的小直径血管移植物。我们认为，天然I型胶原蛋白和弹性蛋白纤维组件的分子结构和超分子组织为设计由生物启发的蛋白质纤维类似物组成的动脉替代品建立了重要的范式。具体来说，我们打算： （1）确定影响基于蛋白质的纤维网络的机械行为和生理学特性的胶原蛋白和弹性纤维类似物的分子水平特征。弹性蛋白和胶原蛋白类似物将由生物合成和化学方案产生，并通过纳米制作技术加工成纤维网络。 解决方案和固态方法都将用于定义材料结构 - 特质关系。 （2）确定蛋白质纤维增强生物聚合物复合材料的微尺度特征，该复合物复合材料决定了与动脉替代品设计相关的机械响应。单一和多组分纤维网络复合材料的机械行为将通过生理相关条件下的静态和动态机械测试研究。在此过程中，构型数学模型将用于协助导管设计，以及随后对构造重塑的分析。 （3）定义体内胶原蛋白和弹性纤维增强血管构建体的形态和结构重塑。两种细胞构建体，以及用自源内皮细胞播种的辅导构建物将在狒狒动物模型中检查。具体而言，将定义局部生物学和机械环境对短期和长期导管特性的影响，包括通畅和生物稳定性。 摘要摘要：优化机械和其他与生物学相关的特性可能是发展对心脏，塑料和血管手术领域至关重要的小直径动脉假体的重要步骤系统。