Metabolomics-Measured Urinary Metabolites, Diet & BP, 17 Population Samples: INTE

代谢组学测量的尿液代谢物、饮食

• 批准号: 7564669
• 负责人: JEREMIAH STAMLER
• 金额: $ 52.52万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7564669
• 关键词: Address; Adult; Age; Albuminuria; Amino Acids; Arachidonic Acids; Area; Arts; Atherosclerosis; Beverages; Biochemical; Bioinformatics; Biometry; Blinded; Blood Pressure; Body mass index; Chicago; Chromatography; Collection; Country; Creatinine; Data; Diet; Dimensions; Economics; Education; Eicosanoids; Electrolytes; England; Ethnic Origin; Excretory function; Food; Gender; Hand; Hour; Hypertension; Individual; Inflammation; Intake; International; Japan; Knowledge; Laboratories; Lead; Life Style; London; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Methods; National Heart, Lung, and Blood Institute; Nuclear Magnetic Resonance; Nutrient; Nutritional; Participant; Pathway interactions; Pattern; Persons; Pharmaceutical Preparations; Phase; Population; Population Heterogeneity; Prevention strategy; Quality Control; Questionnaires; Research; Sampling; Secure; Soy Proteins; Specimen; Surveys; Taiwan; Techniques; Testing; Thrombosis; Time; Urine; Vegetable Proteins; Vegetables; Woman; base; computerized; cost effective; design; lifestyle factors; men; metabolomics; novel; trait; urinary

DESCRIPTION (provided by applicant): BACKGROUND: During 1997-99, the INTERMAP Study - with NHLBI support - surveyed 4,680 men and women ages 40-59 from 17 diverse population samples in Japan (4 samples), the Peoples Republic of China (PRC) (3 samples), the UK (2 samples), and the USA (8 samples). With standardized, quality-controlled methods, 4 24-hour dietary recalls, 2 timed 24-hour urine collections, 8 blood pressure (BP) measurements, anthropometric, and questionnaire data (on sociodemographic and biomedical traits) were accrued for all participants. Foods, beverages, and supplements were converted to nutrients (83 total) with use of up-dated, enhanced, standardized tables on nutrient composition (4 tables, 1/country). Urines were analyzed for Na, K, Ca, Mg, creatinine, albuminuria, amino acids. A high-quality central laboratory in London, England completed nuclear magnetic resonance (NMR) spectroscopic analyses of all urine specimens (2 from each of the 4, 680 participants plus 10% randomly selected, blinded split specimens for external quality control). All data underwent extensive quality control checks and were computerized and secured at the two INTERMAP International Coordinating Centers in Chicago and London, England. AIMS: Overall aim is - in a highly cost effective way - to add a new dimension of research information and thereby achieve a qualitative advance in knowledge on the etiopathogenesis of population-wide adverse BP levels (prehypertensive and hypertensive), i.e., develop a new epidemiologic area based on the urinary metabolic products whereby multiple dietary and diet-related factors influence BP. Based on the vast, unique array of high quality BP, nutritional, urinary metabolite, etc. data available and accruable, the specific aims are to identify and quantify - from NMR spectra in hand plus mass spectrometry and other techniques - urinary metabolites discriminatively related to BP and to each of several dietary (vegetable protein, soy protein, arachidonic acid, Na/K), diet-related sociodemographic (education, ethnicity) and anthropometric (body mass index) factors impacting BP. Advanced bioinformatics techniques and biochemical methods are to be used to address each aim. SIGNIFICANCE: The BP problem is population-wide; waiting for individuals to develop hypertension and then treating them with drugs is an inadequate strategy. This study - by identifying specific urinary metabolites implicated in the etiologic pathways whereby adverse dietary/lifestyle patterns lead to population-wide adverse BP levels - can help create fully effective strategies for prevention/eradication of the mass BP problem.

描述（由申请人提供）：背景：在1997 - 99年期间，Intermap研究 - 在NHLBI支持的情况下 - 在日本的17种不同人口样本（4个样本）（PRC）（PRC）（3个样本）（3个样本），英国（2个样本）和美国（8个样品）（8个样本）和美国（8个样品）中，接受了4,680名40-59岁的男女40-59岁的男女。通过标准化的质量控制方法，4个24小时的饮食召回，2个定时的24小时尿液收集，8个血压（BP）测量，人体测量值和问卷数据（有关社会人口统计学和生物医学特征）。食物，饮料和补充剂在营养成分（4桌子，1/country）上使用上的，增强的，标准的表，将食物，饮料和补充剂转化为营养（总计83）。分析了Na，K，Ca，Mg，肌酐，蛋白尿，氨基酸的尿液。英格兰伦敦的高质量中央实验室完成了所有尿液标本的核磁共振（NMR）光谱分析（来自4，680名参与者中的每个标本中的2个，以及10％随机选择的，盲目的盲型样品以进行外部质量控制）。所有数据都进行了广泛的质量控制检查，并在芝加哥和英国伦敦的两个Intermap国际协调中心进行了计算机化和确保。目的：总体目标是 - 以一种高度成本效益的方式 - 添加研究信息的新维度，从而在人口范围内的不良BP水平（型和高血压前和高血压）的疗效知识上取得了定性的进步，即，基于多种饮食饮食和饮食饮食和饮食和饮食和饮食和饮食和饮食和饮食和饮食和饮食和饮食和利益因素的幼发代谢产物而开发出新的流行病学领域。 Based on the vast, unique array of high quality BP, nutritional, urinary metabolite, etc. data available and accruable, the specific aims are to identify and quantify - from NMR spectra in hand plus mass spectrometry and other techniques - urinary metabolites discriminatively related to BP and to each of several dietary (vegetable protein, soy protein, arachidonic acid, Na/K), diet-related sociodemographic （教育，种族）和人体测量学（体重指数）影响BP的因素。先进的生物信息学技术和生化方法应用于解决每个目标。意义：BP问题在范围内；等待个人发展高血压，然后用药物治疗它们是一种不足的策略。这项研究 - 通过确定与病因学途径有关的特定泌尿代谢产物，从而导致饮食/生活方式模式导致范围内的不良BP水平 - 可以帮助创建完全有效的策略，以预防/消除大众BP问题。