Gene Targeting Core Facility

基因打靶核心设施

基本信息

批准号：
7352462
负责人：
ROBERT A KESTERSON
金额：
$ 8.27万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-01 至 2012-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7352462
关键词：
Animal Experimentation Animal Model Animals Biological Models Breeding Cancer Center Clinical Nutrition Consultations Core Facility Cryopreservation DNA Disease ES Cell Line Embryo Functional disorder Gene Targeting Genetic Genetically Engineered Mouse Genomics Goals Human Human Resources Image Immune system Individual Injection of therapeutic agent Intervention Laboratories Link Microinjections Mission Modification Molecular Mus Nutritional Study Pathology Phenotype Postdoctoral Fellow Production Productivity Reagent Research Research Personnel Resources Rheumatism Services Stem Cell Development Students System Technology Tissues Training Transgenes Transgenic Animals Transgenic Mice Transgenic Mouse Facility Transgenic Organisms University of Alabama at Birmingham Cancer Center animal facility blastocyst cost cost effective design embryonic stem cell genetic manipulation human disease interest knockout gene lectures mouse genome mouse model recombinase reconstitution vector

项目摘要

The Gene Targeting Core Facility (GTCF) is a fully established core resource to provide expert services for gene targeting of embryonic stem (ES) cells for the development of mouse models. The primary service orovided by this core is that of taking an investigator's gene targeting vector and producing ES cell lines that can be developed into chimeric mice that transmit the pertinent genomic modification(s) through their germline. Investigators utilizing this core have successfully produced a variety of gene knockouts and mouse models of disease. The GTCF is an integral component of the UAB Transgenic Mouse Facility that collectively provides expert services for not only gene targeting via the GTCF, but also blastocyst micro-injection of targeted ES cell clones or DNA constructs for the production of transgenic mouse models, as well as a host of services related to research with mice (e.g. cryopreservation of embryos, rederivation, etc.). The Transgenic Animal Facility is strategically linked with other core resources at UAB including the CCC Small Animal Imaging Core, Clinical Nutrition Research Center Small Animal Phenotyping Core, Research Animal Pathology Laboratory for phenotype assessment, and a specialized mouse-breeding core (Genetically Engineered Mouse and Marker-Assisted Genetics Core). Overall, we have premier resources to make and characterize mouse models for the Center investigators.

基因打靶核心设施 (GTCF) 是一个完全成熟的核心资源，旨在为用于开发小鼠模型的胚胎干（ES）细胞的基因靶向。主要服务该核心提供的方法是获取研究者的基因靶向载体并产生 ES 细胞系，可以发育成嵌合小鼠，通过其种系传递相关的基因组修饰。研究人员利用该核心已成功生产出多种基因敲除小鼠疾病模型。 GTCF 是 UAB 转基因小鼠设施的一个组成部分，不仅通过 GTCF 共同提供基因打靶专家服务，还为囊胚提供专家服务显微注射目标 ES 细胞克隆或 DNA 构建体，用于生产转基因小鼠模型，以及与小鼠研究相关的一系列服务（例如胚胎冷冻保存、再衍生、 ETC。）。转基因动物设施与 UAB 的其他核心资源具有战略联系，包括 CCC 小动物影像核心、临床营养研究中心小动物表型核心、用于表型评估的研究动物病理学实验室和专门的小鼠育种核心（基因工程小鼠和标记辅助遗传学核心）。总体而言，我们拥有一流的资源为中心研究人员制作并表征小鼠模型。