GENE EXPRESSIONS AND GENOMIC ANALYSIS CORE

基因表达和基因组分析核心

• 批准号: 7490694
• 负责人: SHERMAN Morton WEISSMAN
• 金额: $ 22.66万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7490694
• 关键词: Address; Antibodies; Arts; Biological Assay; Cellular biology; Chromatin; Chromosomes, Human, Pair 22; Computer Workstations; DNA Microarray Chip; DNA Microarray format; Data Analyses; Detection; Diagnosis; Emerging Technologies; Evaluation; Funding; Gene Expression; Genes; Genomics; Glass; Hand; Hematological Disease; Hematology; Hematopoiesis; Hematopoietic; Human; Laboratories; Microarray Analysis; Molecular Analysis; Polymerase Chain Reaction; Proteomics; Range; Relative (related person); Research; Research Personnel; Sensitivity and Specificity; Services; Site; Slide; Spottings; Stem cells; Techniques; Technology; Time; Training; Training and Education; Transcript; Work; base; chromatin immunoprecipitation; instrument; instrumentation; interest; novel; prevent; promoter; research study; tool

The Gene Expression and Genomic Analyses Core will provide access to and training in emerging technologies for molecular analysis of hematopoiesis and stem cell biology to investigators of the YCEMH. First, the Core will provide services in Gene Expression Microarray Technology including providing comprehensive, state-of-the-art Affymetrix and spotted glass slide microarray analyses, providing DNA microarray training and education via training seminars, one-on-one training sessions, and ongoing advice and guidance in the use of microarrays for a range of experiments including transcript analysis, ChlP-chip, and other applications, and by providing specialized services, evaluation and optimization of the application of microarray analysis to solve challenging hematopoiesis/stem cell biology research problems. One specific challenge is the evaluation of different microarray platforms for relative detection sensitivity and specificity for low abundance hematopoiesis-related genes. Second, the Core will provide services related to Genomics and Microarray Technology by providing novel microarrays, including genomic tiling arrays, the ABI 1700 DNA microarray, the human chromosome 22 array, and the human promoter array, to investigators of the YCEMH, by providing microarray training and education for use of these specialized microarrays, and by providing specialized services in array technology for the evaluation and optimization of genomic microarray technologies to researchers in hematopoiesis and stem cell biology. This includes access to an on-site microarray hybridization chamber and a dedicated computer workstation equipped for hematology/stem cell-related array data analyses. In addition, the Core will provide training, education, and ready access to relevant laboratory instrumentation for chromatin immunoprecipitation including hands-on training in ChIP and ChlP-chip experiments. Access to an ABI Real-Time iCycler PCR instrument will provide investigators necessary tools to perform quantitative ChlP-related assays. This Core will also maintain an up-to-date profile of antibodies useful in ChIP with emphasis on those validated by the Yale Proteomics Center for distribution to interested investigators. Providing the most advanced microarray and chromatin-based technologies to YCEMH investigators will facilitate discoveries in hematology/stem cell biology that advance our ability to understand, diagnose, treat, and prevent hematological diseases.

基因表达和基因组分析核心将为 YCEMH 的研究人员提供造血和干细胞生物学分子分析新兴技术的访问和培训。首先，核心将提供基因表达微阵列技术服务，包括提供全面、最先进的 Affymetrix 和斑点载玻片微阵列分析，通过培训研讨会、一对一培训课程和在使用微阵列进行一系列实验（包括转录物分析、ChlP 芯片和其他应用）方面提供持续的建议和指导，并提供专业服务、应用评估和优化 微阵列分析解决具有挑战性的造血/干细胞生物学研究问题。一项具体的挑战是评估不同微阵列平台对低丰度造血相关基因的相对检测灵敏度和特异性。其次，核心将通过向 YCEMH 的研究人员提供新型微阵列，包括基因组平铺阵列、ABI 1700 DNA 微阵列、人类 22 号染色体阵列和人类启动子阵列，来提供与基因组学和微阵列技术相关的服务。使用这些专用微阵列的培训和教育，并提供阵列技术的专业服务以进行评估和优化 为造血和干细胞生物学研究人员提供基因组微阵列技术。这包括使用现场微阵列杂交室和配备用于血液学/干细胞相关阵列数据分析的专用计算机工作站。此外，核心还将提供培训、教育以及染色质免疫沉淀相关实验室仪器的使用，包括 ChIP 和 ChlP 芯片实验的实践培训。使用 ABI 实时 iCycler PCR 仪器将为研究人员提供执行定量 ChlP 相关检测所需的工具。该核心还将维护 ChIP 中有用的抗体的最新概况，重点是经耶鲁大学蛋白质组学中心验证的抗体，以分发给感兴趣的研究人员。为 YCEMH 研究人员提供最先进的微阵列和基于染色质的技术将促进血液学/干细胞生物学的发现，从而提高我们理解、诊断、治疗和预防血液疾病的能力。