GENE EXPRESSIONS AND GENOMIC ANALYSIS CORE

基因表达和基因组分析核心

• 批准号: 7490694
• 负责人: SHERMAN Morton WEISSMAN
• 金额: $ 22.66万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7490694
• 关键词: Address; Antibodies; Arts; Biological Assay; Cellular biology; Chromatin; Chromosomes, Human, Pair 22; Computer Workstations; DNA Microarray Chip; DNA Microarray format; Data Analyses; Detection; Diagnosis; Emerging Technologies; Evaluation; Funding; Gene Expression; Genes; Genomics; Glass; Hand; Hematological Disease; Hematology; Hematopoiesis; Hematopoietic; Human; Laboratories; Microarray Analysis; Molecular Analysis; Polymerase Chain Reaction; Proteomics; Range; Relative (related person); Research; Research Personnel; Sensitivity and Specificity; Services; Site; Slide; Spottings; Stem cells; Techniques; Technology; Time; Training; Training and Education; Transcript; Work; base; chromatin immunoprecipitation; instrument; instrumentation; interest; novel; prevent; promoter; research study; tool

The Gene Expression and Genomic Analyses Core will provide access to and training in emerging technologies for molecular analysis of hematopoiesis and stem cell biology to investigators of the YCEMH. First, the Core will provide services in Gene Expression Microarray Technology including providing comprehensive, state-of-the-art Affymetrix and spotted glass slide microarray analyses, providing DNA microarray training and education via training seminars, one-on-one training sessions, and ongoing advice and guidance in the use of microarrays for a range of experiments including transcript analysis, ChlP-chip, and other applications, and by providing specialized services, evaluation and optimization of the application of microarray analysis to solve challenging hematopoiesis/stem cell biology research problems. One specific challenge is the evaluation of different microarray platforms for relative detection sensitivity and specificity for low abundance hematopoiesis-related genes. Second, the Core will provide services related to Genomics and Microarray Technology by providing novel microarrays, including genomic tiling arrays, the ABI 1700 DNA microarray, the human chromosome 22 array, and the human promoter array, to investigators of the YCEMH, by providing microarray training and education for use of these specialized microarrays, and by providing specialized services in array technology for the evaluation and optimization of genomic microarray technologies to researchers in hematopoiesis and stem cell biology. This includes access to an on-site microarray hybridization chamber and a dedicated computer workstation equipped for hematology/stem cell-related array data analyses. In addition, the Core will provide training, education, and ready access to relevant laboratory instrumentation for chromatin immunoprecipitation including hands-on training in ChIP and ChlP-chip experiments. Access to an ABI Real-Time iCycler PCR instrument will provide investigators necessary tools to perform quantitative ChlP-related assays. This Core will also maintain an up-to-date profile of antibodies useful in ChIP with emphasis on those validated by the Yale Proteomics Center for distribution to interested investigators. Providing the most advanced microarray and chromatin-based technologies to YCEMH investigators will facilitate discoveries in hematology/stem cell biology that advance our ability to understand, diagnose, treat, and prevent hematological diseases.

基因表达和基因组分析核心将为YCEMH研究者提供对造血和干细胞生物学分子分析的新兴技术的访问和培训。首先，核心将提供基因表达微阵列技术的服务，包括提供全面的，最先进的Affymetrix和Spotted Slide Microaray分析，通过培训研讨会，一对一的培训课程，一对一的培训课程，以及在范围的范围内使用范围的实验分析，提供DNA微阵列培训和教育应用程序的服务，评估和优化 微阵列分析以解决具有挑战性的造血/干细胞生物学研究问题。一个具体的挑战是评估不同微阵列平台的相对检测灵敏度和低丰度造血相关基因的特异性。第二，核心将通过提供新颖的微阵列，包括基因组瓷砖阵列，ABI 1700 DNA微阵列，人类22号人类染色体阵列和人类启动子阵列来提供与基因组学和微阵列技术有关的服务 造血和干细胞生物学研究人员的基因组微阵列技术。这包括访问现场微阵列杂交室以及配备血液学/与干细胞相关阵列数据分析的专用计算机工作站。此外，该核心将提供培训，教育和现成的相关实验室仪器，以进行染色质免疫沉淀，包括芯片和CHLP-CHIP实验中的动手培训。使用ABI实时ICYCLER PCR仪器将为调查人员提供必要的工具，以执行与CHLP相关的定量相关测定。该核心还将维护有用的抗体的最新曲线，重点是由耶鲁大学蛋白质组学中心验证的抗体，向有兴趣的研究者分发。向YCEMH研究者提供最先进的微阵列和基于染色质的技术，将促进血液学/干细胞生物学的发现，以提高我们理解，诊断，治疗和预防血液学疾病的能力。