GENETIC MANAGEMENT OF ANIMAL COLONIES

动物群体的遗传管理

基本信息

批准号：
7575839
负责人：
DAVID Glenn SMITH
金额：
$ 27.86万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-09-30 至 2009-02-08
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7575839
关键词：
Accounting Advertising Alleles Animal Model Animals Anthropology Applications Grants Appointment Area Asia Asians Authorization documentation Base Sequence Biological Biomedical Research Breeding California Characteristics China Chinese People Chromosomes Class Cloning Colorado Computer software Count Country DNA Data Data Analyses Databases Disclosure Doctor of Philosophy Educational process of instructing Equipment and supply inventories Exclusion Face Facility Construction Funding Category Genealogy Genes Genetic Genetic Heterogeneity Genetic Markers Genetic Variation Genotype Geographic Locations Goals Grant Haplotypes Health Services Accessibility Human Resources Hybrids Immune Inbreeding Individual Indonesia Instruction Knowledge Laboratories Last Name Link MHC Class II Genes Macaca Macaca mulatta Maintenance Malaysia Medicine Methodology Methods Mitochondria Mitochondrial DNA Modeling Monkeys Myanmar Names National Center for Research Resources Nuclear Numbers Other Genetics Pakistan Performance Personnel Staffing Phenotype Phylogenetic Analysis Policies Polymerase Population Population Genetics Population Sizes Population Study Positioning Attribute Postdoctoral Fellow Primates Principal Investigator Printing Probability Procedures Publishing Purpose Qi RNA, ribosomal, 12S Range Recommendation Recording of previous events Relative (related person)Reporting Research Research Project Grants Restriction fragment length polymorphism Ribosomal RNA Role Sampling Services Sister Site Standards of Weights and Measures Supervision Tail Taxon Testing Time Trees United States National Institutes of Health Universities Vietnam Visit Wages Work Y Chromosome animal colony authority base cohort computer program day design experience father role genetic analysis genetic pedigree genetic profiling human disease improved male nonhuman primate programs reproductive success size southeast Asian trait

项目摘要

The genotypes or phenotypes of geographically representative samples of rhesus macaques, Macaca mulatta, of different subspecies of Chinese, Indian and southeast Asian origin will be compared in phylogenetic context (i.e., together with those of six other species of macaques) using approximately 75 DNA loci, amplified using the polymerase chain recation (PCR), to genetically characterize these taxa These genetic differences will be compared among various hierarchical levels (e.g. regional populations, subspecies, countries of origin, species) of macaques to assess their biological significance using gene diversity and phylogenetic methods and each taxa will be genetically characterized with specific marker alleles relative to each other. The loci to be studied include neutral (STR) loci widely dispersed over all chromosomes, mt DNA, a Y-chromosome haplotype,and both class I and class II MHC loci which influence immune responsiveness. A program to assign samples of unknown provenience to their correct subspecies, geographic region or country of origin, now in use, will be substantially improved using the new population samples and genetic data generated by this grant. Services provided to all nonhuman primate breeding facilities by this grant include typing rhesus macaques using 15 most informative STR loci to establish pedigrees, the use of these data to estimate genetic parameters such as effective population size, inbreeding and average kinship coefficients, gene diversity, generate a genetic profile of individual animals, conduct paternity exclusion analysis for estimating relative reproductive success of male breeders and specific founding lineages and the use of the aforementioned assignment program and taxa-specific genetic markers identified during the course of this study to identify the country of origin and subspecies of samples of unknown origin. In addition, specific recommendations tailored to the unique facilities at each SPF breeding colony supported by NIH will be prepared and provided to each colony to facilitate maintenance of genetic heterogeneity.

将使用大约 75 个 DNA 位点，在系统发育背景下（即与其他六种猕猴一起）对中国、印度和东南亚不同亚种的恒河猴（Macaca mulatta）地理代表性样本的基因型或表型进行比较，使用聚合酶链反应（PCR）进行扩增，以对这些分类群进行遗传表征。这些遗传差异将在不同的层次水平（例如，猕猴的区域种群、亚种、原产国、物种），利用基因多样性和系统发育方法评估其生物学意义，每个类群将通过彼此相关的特定标记等位基因进行遗传表征。要研究的基因座包括广泛分散在所有染色体上的中性（STR）基因座、线粒体DNA、Y染色体单倍型以及影响免疫反应的I类和II类MHC基因座。目前正在使用的一项将来源不明的样本分配给正确的亚种、地理区域或原产国的计划将利用这笔赠款产生的新种群样本和遗传数据得到大幅改进。这笔赠款向所有非人类灵长类动物繁育设施提供的服务包括使用 15 个信息最丰富的 STR 位点来建立系谱，对恒河猴进行分型，使用这些数据来估计遗传参数，例如有效种群规模、近亲繁殖和平均亲缘系数、基因多样性、生成个体动物的遗传图谱，进行亲子排除分析，以估计雄性育种者和特定创始谱系的相对繁殖成功率，以及使用上述分配程序和分类群特异性遗传标记在本研究过程中确定了来源不明的样本的原产国和亚种。此外，还将针对 NIH 支持的每个 SPF 育种群体的独特设施制定具体建议，并将其提供给每个群体，以促进遗传异质性的维持。