Differential Interference Contrast Optofluidic Microscopy

微分干涉光流控显微镜

• 批准号: 7513398
• 负责人: CHANGHUEI YANG
• 金额: $ 19.94万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7513398
• 关键词: Basic Science; Caenorhabditis elegans; Cells; Development; Devices; Elements; Image; Imaging Techniques; Methods; Microfluidics; Microscope; Microscopy; Nomarski Interference Contrast Microscopy; Optics; Pattern; Performance; Phase; Principal Investigator; Procedures; Range; Research; Resolution; Standards of Weights and Measures; System; Technology; Work; Yang; base; cost; design; desire; micro-total analysis system; microorganism; programs; prototype; research study

DESCRIPTION (provided by applicant): This Exploratory/Developmental Research (R21) application proposes the development of an on-chip and low cost phasing imaging microscope that can significantly simplify a broad range of existing biomedicine analysis procedures. This imaging approach, termed differential interference contrast optofluidic microscopy (DIC-OFM), will build upon our work on the optofluidic microscopy (OFM) - a high resolution, lensless and compact on-chip microscope method. Our preliminary experiments positively indicate that a low cost, simple phase imaging approach based on the use of patterned aperture clusters, that function as on-chip Young's double slit interferometers, can render differential interference contrast (DIC) microscopy type images without the use of elaborate bulk optical elements. Such an on-chip and low cost phase microscope method implies the possibility of high throughput and massively serial/parallel imaging of bio-entities. Among other uses, it can be applied in microfluidic based cell or microorganism analysis, and any other type of lab-on-a-chip applications where a compact and low cost phase imaging system is desired. We aim to demonstrate a working device that is capable of high throughput imaging with a spatial resolution of at least 1.2 micron (comparable to a 10x standard microscope) and quantitative spatial differential phase sensitivity of ~5 degree/micron. With the completion of the proposed research, we expect the technology will be sufficiently refined to be applied to tackle a wide range of basic research and biomedicine challenges. The specific aims of the proposal are as follows: 1. To construct a relay image geometry DIC-OFM prototype. 2. To construct a fully integrated DIC-OFM prototype. 3. To study and evaluate the prototypes' performance for rendering phase images of C. elegans. 4. To examine other DIC-OFM aperture configurations. The proposed research will allow us to investigate the possibility of constructing on-chip, low cost and high resolution differential interference contrast (DIC) microscopes. The propose microscope design builds upon our successful optofluidic microscope work. A compact and low cost DIC optofluidic microscope system can dramatically simplify phase imaging procedure and introduce phase imaging to a wider range of biomedical applications, which are presently hindered by the high cost of conventional DIC microscopes.

描述（由申请人提供）：探索/发展研究（R21）申请提出了开发片上和低成本的相位相分化成像显微镜的开发，该显微镜可以显着简化一系列现有的生物医学分析程序。这种被称为差分干扰对比度荧光显微镜（DIC-OFM）的成像方法将基于我们在光荧光显微镜（OFM）上的工作 - 高分辨率，无透镜和紧凑的芯片显微镜方法。我们的初步实验积极地表明，基于模式的孔径簇的使用，低成本，简单的相像方法，该方法充当了芯片Young的双缝线干涉仪，可以使微分干扰对比度（DIC）显微镜类型的图像无需使用精确的散装光学元素。这种片上和低成本阶段显微镜方法意味着生物本性的高吞吐量和大量串行/平行成像的可能性。除其他用途外，它可以应用于基于微流体的细胞或微生物分析，以及任何其他类型的实验室芯片应用程序，其中需要紧凑型和低成本阶段成像系统。我们的目的是展示一种能够具有高吞吐量成像的工作装置，其空间分辨率至少为1.2微米（与10倍标准显微镜相当）和〜5度/微米的定量空间差异相位灵敏度。随着拟议的研究的完成，我们预计该技术将得到充分精炼，以应对广泛的基础研究和生物医学挑战。该提案的具体目的如下：1。构建继电器图像几何原型。 2。构建完全集成的DIC-OFM原型。 3。研究和评估原型的表演性能，以渲染秀丽隐杆线虫的相位图像。 4。检查其他DIC-OFM孔径配置。拟议的研究将使我们能够研究构建片上芯片，低成本和高分辨率差分干扰对比（DIC）显微镜的可能性。提出的显微镜设计建立在我们成功的Optofluidic显微镜工作的基础上。紧凑型和低成本的DIC Optofluidic显微镜系统可以极大地简化相成像过程，并将相成像引入更广泛的生物医学应用，目前，这些应用程序受到传统DIC显微镜的高成本的阻碍。