CORE C-- BIOMARKERS OF OXIDATIVE STRESS

核心 C——氧化应激的生物标志物

• 批准号: 7454289
• 负责人: Valerian E Kagan
• 金额: $ 14.05万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7454289
• 关键词: 4-anisyltetrazolium blue; 8-hydroxy-2' -deoxyguanosine; A549; Acetates; Acetic Acid; Acetic Acids; Acetone; Acetonitriles; Acids; Acridines; Activated Charcoal; Acute Lung Injury; Affect; Aftercare; Albumins; Aliquot; Alkaline Phosphatase; Animals; Antioxidants; Appearance; Area; Area Under Curve; Arts; Ascorbic Acid; Azides; Bee Venoms; Binding; Biological; Biological Assay; Biological Markers; Biological Models; Biological Sciences; Biotin; Biotinylation; Boston; Brain; Buffers; Butylated Hydroxytoluene; Caliber; Calibration; Carbon; Cardiolipins; Cell Survival; Cell membrane; Cell model; Cells; Centrifugation; Cerebrospinal Fluid; Characteristics; Chemiluminescence assay; Chloroform; Class; Clinical; Complex; Computer software; Condition; Coomassie brilliant blue R; Coumarins; Culture Media; Cultured Cells; Custom; Cysteine; Cytolysis; DNA; DNA Modification Process; Data; Deoxyguanosine; Deoxyribonuclease I; Dependence; Detection; Detergents; Development; Dialysis procedure; Diazomethane; Diethyldithiocarbamate; Digestion; Dimethyl Sulfoxide; Dinoprost; Doxorubicin; Doxycycline; Dyes; Edetic Acid; Electrodes; Electron Spin Resonance Spectroscopy; Electronics; Electrons; Endothelial Cells; Enhancers; Enzymes; Epithelial; Epithelial Cells; Equilibrium; Equipment; Esterified Fatty Acids; Esters; Ethanol; Ethidium; Etoposide; Evaluation; Exposure to; FGF7 gene; Fatty Acids; Fiber Optics; Figs - dietary; Film; Finland; Fluorescence; Fluorescence Microscopy; Free Radicals; Freezing; Gases; Gel; Generations; Genes; Glass; Glucose; Glutathione; HEPES; HL-60 Cells; Heating; Hexanes; High Pressure Liquid Chromatography; High temperature of physical object; Horseradish Peroxidase; Hour; Human; Hydrochloride Salt; Hydrogen; Hydrogen Peroxide; Hydrolysis; Hydroxyl Radical; Hyperoxia; Ice; Image; Immunoblotting; Incubated; Individual; Industrial Product; Injection of therapeutic agent; Injury; Instruction; Isopropanol; Japan; Kinetics; Knockout Mice; L-Ascorbate oxidase; Label; Laboratories; Lasers; Lecithin; Left; Length; Life; Light; Link; Lipid Peroxidation; Lipids; Lipoxygenase; Liquid substance; Longevity; Luminol; Lung; Maleimides; Manufacturer Name; Measurement; Measures; Membrane; Metabolic; Metals; Methanol; Methodology; Methods; Microscope; Microscopy; Minor; Modeling; Modification; Molecular; Molecular Probes; Molecular Sieve Chromatography; Molecular Weight; Monitor; Monoclonal Antibodies; Monophenol Monooxygenase; Mus; Myristates; NADH; NBL1 gene; NOC-15; Natural regeneration; Necrosis; Nitrogen; Nonesterified Fatty Acids; Nuclease P1; Numbers; Operant Conditioning; Output; Oxidants; Oxidation-Reduction; Oxidative Stress; Oxides; Oxygen; Oxygenases; PAPA NONOate; PTEN gene; Particle Size; Performance; Peroxidase; Peroxidases; Phase; Phenols; Phenolsulfonphthalein; Phorbol; Phorbols; Phosphate Buffer; Phosphatidylinositols; Phospholipase A2; Phospholipids; Phosphorus; Physical Dialysis; Plasma; Potassium Phosphate; Preparation; Principal Investigator; 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Vertebrates; Vitamin E; Water; Welts; Width; Wild Type Mouse; Work; adduct; alpha benzopyrone; alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone; antitumor drug; aqueous; ascorbate; attenuation; autooxidation; base; biochemical model; brain tissue; catalase; cell type; cellular imaging; charge coupled device camera; coral; cyclooxygenase 1; cyclooxygenase 2; data acquisition; design; detector; diene; digital; dihydroethidium; dinitrosyl iron complex; ethyl acetate; experience; fetal; fluorescence microscope; gel electrophoresis; human subject; immunoreactivity; in vivo; inhibitor/antagonist; inorganic phosphate; instrument; instrumentation; irradiation; isoprostaglandin F2alpha type-III; keratinocyte growth factor; lung injury; macrophage; microwave electromagnetic radiation; mind control; myristate; neocuproine; nitrone; novel; oxidation; paraform; parinaric acid; perhydroxyl radical; peroxidation; phenoxy radical; phosphoric diester hydrolase; piperidine; polyacrylamide gels; prevent; programs; purge; reconstitution; repaired; research study; resorufin; response; sephadex G-75; sodium phosphate; tetrahydrofuran; time interval; tissue culture; trend; two-photon; ubiquinol; ultrasphere-octadecyltrimethyloxysilane; ultraviolet irradiation

One of the central topics of this Program Project (PO1) is that acute lung injury is associated with increased production of reactive oxygen species that may be responsible, at least in part, for oxidative injury to pulmonary cells. This suggests that monitoring free radicals, biomarkers of oxidative stress and antioxidant status is essential for understanding the mechanisms of acute lung injury and developing novel optimized therapeutic strategies. The assays of oxidative stress will be performed in the core on Biomarkers of Oxidative Stress (COBOS). The core has been designed to allow researchers in the projects to measure biomarkers of oxidative stress using sophisticated state-of-the art techniques to assess several of the different relevant parameters. Specific Aims of the core are: 1. Provide professional expertise in the design and implementation of experiments using adequate techniques for assessment of biomarkers of oxidative stress and antioxidants. 2. Prepare, store and optimally analyze samples of tissues, cells and biological fluids to detect free radical intermediates, as well as biomarkers of oxidative stress in major classes of biomolecules (proteins, DNA, and lipids). 3. Evaluate experimental results and propose subsequent experimental direction. 4. Provide training in the use of and access to any instrumentation and techniques used with the program project to assess biomarkers of oxidative stress and antioxidant status. Overall, COBOS will be central to the planning and execution of experiments on oxidative stress md antioxidants within the program.

该计划项目（PO1）的中心主题之一是急性肺损伤与 活性氧的产生增加，可能至少部分导致氧化损伤 肺细胞。这表明监测自由基、氧化应激和抗氧化状态的生物标志物 对于了解急性肺损伤的机制和开发新的优化治疗方法至关重要 策略。 氧化应激检测将在氧化应激生物标志物 (COBOS) 的核心中进行。 该核心的设计目的是让项目中的研究人员能够使用以下方法测量氧化应激的生物标志物： 先进的技术来评估几个不同的相关参数。 核心的具体目标是： 1. 提供实验设计和实施方面的专业知识 评估氧化应激和抗氧化剂生物标志物的适当技术。 2. 制备、储存和优化分析组织、细胞和生物液体样本，以 检测自由基中间体以及主要氧化应激的生物标志物 生物分子类别（蛋白质、DNA 和脂质）。 3.评估实验结果并提出后续实验方向。 4. 提供使用和获取任何仪器和技术的培训 该计划旨在评估氧化应激和抗氧化状态的生物标志物。 总体而言，COBOS 将成为氧化应激实验规划和执行的核心 程序内的 MD 抗氧化剂。