Research Irradiator

研究辐照器

• 批准号: 7046427
• 负责人: Lester William Cashdollar
• 金额: $ 32.38万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2008-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046427
• 关键词: blood; role

DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of The Blood Center of Southeastern Wisconsin has an established, long-term commitment to blood-related research. More than 15 Principle Investigators lead research programs in the fields of cardiovascular biology, immunology, cancer biology, control of bleeding and thrombosis and transfusion medicine. Many of these research programs utilize transgenic, knockout and normal mice in various models of human diseases. Experiments performed with these animals often require partial or lethal irradiation using a gamma irradiator. Because of the increased use of mice by BRI scientists and the lack of research animal space on the Milwaukee Regional Medical campus the Blood Center has entered into an agreement with the Medical College of Wisconsin to house the mice needed by BRI scientists in the new MCW Biomedical Resource Center. A gamma irradiator will be needed as shared equipment in this new animal facility. We request funds in this application to purchase a research gamma irradiator. Projects initiated by fourteen NIH-funded investigators and those initiated by one new investigator require this instrument to accomplish their research goals. These project titles are: Structure and Function of VWFpp; Molecular and Cellular Consequences of vWF Alteration; Robust T cell Immunity to Influenza in Human Populations; Molecular Mechanisms of Platelet Activation and Adhesion; Molecular Biology and Function of PECAM-1; Endothelial Redox Signaling Mediated by PECAM-1; Mechanisms of Vaso-Occlusion in Sickle Cell Disease: role of thrombosis; Red Blood Cell Adhesion in Sickle Cell Disease; Individual and Collaborative Roles of PLCyl and PLCy2 in T cell Activation and Development; StatS Dephosphorylation by Shp-2; Bone Marrow Derived Allo-NK Cells for Tumor Targeted Immunotherapy; The Role of Cryptic Epitopes in CD8+ T cell Mediated Tumor Clearance; Novel Function of an Important MH-Ag, H60 during GvL; Encephalitogenic T Cell Regulation of Microglial Cells; Investigating the Role of Bcl-x in Leukemogenesis by BCR/ABL; Tissue Factor Pathway Inhibitor Binding Proteins on Endothelium; CD200 Expression on Apoptotic DCs and Immune Tolerance; CD4 Regulatory T Cells Suppress Alloreactivity after BMT; Optimizing Immunotherapy for Neuroblastoma after BMT; Regulation of Engraftment and Immunity by Gamma Delta T Cells; Molecular and Cellular Mechanisms in Transfusion Medicine, Core C, Transgenic Core. A gamma irradiator is an essential piece of shared equipment that is needed for the new MCW animal facility where BRI and MCW Investigator's mice will be housed. It will support the needs of current investigators and will be available to new investigators as they are recruited during the planned expansion of the Blood Research Institute and the Medical of Wisconsin.

描述（由申请人提供）：威斯康星州东南部血液中心的血液研究所 (BRI) 长期致力于血液相关研究。超过 15 名首席研究员领导心血管生物学、免疫学、癌症生物学、出血和血栓形成控制以及输血医学领域的研究项目。其中许多研究项目在各种人类疾病模型中利用转基因、基因敲除和正常小鼠。对这些动物进行的实验通常需要使用伽马辐照器进行部分或致死的辐照。由于 BRI 科学家越来越多地使用小鼠，并且密尔沃基地区医学园区缺乏研究动物空间，血液中心已与威斯康星医学院达成协议，将 BRI 科学家所需的小鼠安置在新的 MCW 生物医学中心资源中心。这个新的动物设施将需要伽马辐照器作为共享设备。我们在此申请中请求资金来购买研究用伽马辐照器。由 14 名 NIH 资助的研究人员发起的项目和由一名新研究人员发起的项目需要该仪器来实现他们的研究目标。这些项目名称是：VWFpp 的结构和功能； vWF 改变的分子和细胞后果；人类对流感具有强大的 T 细胞免疫力；血小板激活和粘附的分子机制； PECAM-1的分子生物学和功能； PECAM-1 介导的内皮氧化还原信号传导；镰状细胞病中血管闭塞的机制：血栓形成的作用；镰状细胞病中的红细胞粘附； PLCyl 和 PLCy2 在 T 细胞激活和发育中的单独和协同作用； Shp-2 的 StatS 去磷酸化；用于肿瘤靶向免疫治疗的骨髓来源的同种异体 NK 细胞；隐性表位在 CD8+ T 细胞介导的肿瘤清除中的作用；重要 MH-Ag H60 在 GvL 期间的新功能；小胶质细胞的致脑炎 T 细胞调节；通过 BCR/ABL 研究 Bcl-x 在白血病发生中的作用；内皮上的组织因子途径抑制剂结合蛋白；凋亡 DC 上的 CD200 表达和免疫耐受； CD4 调节性 T 细胞在 BMT 后抑制同种异体反应性； BMT 后优化神经母细胞瘤的免疫治疗； Gamma Delta T 细胞对植入和免疫的调节；输血医学的分子和细胞机制，核心 C，转基因核心。伽马辐照器是新的 MCW 动物设施所需的重要共享设备，BRI 和 MCW 调查员的小鼠将被安置在该设施中。它将支持当前研究人员的需求，并将提供给在血液研究所和威斯康星州医学中心计划扩建期间招募的新研究人员。